Multi-channel 3D cell invasion and metastasis measuring device

Abstract

The invention discloses a multi-channel 3D cell invasion and metastasis measuring device. The device comprises a matrix glue injection cylinder, a cell invasion and metastasis box, four cell culture boxes and four slide storage boxes, the cell invasion and metastasis box and the four cell culture boxes are all of a cuboid-shaped box body structure, and the tops of the cell invasion and metastasisbox and the four cell culture boxes are open. In conclusion, the matrix glue injection cylinder is used for injecting matrix glue, and four matrix glue solution cavities of the matrix glue injection cylinder are independent of one another and are not communicated with one another, so that the cross influence of different cells or cell factors is prevented. The device is convenient to use, can be used for simultaneously determining the influence of cells or different cell factors in a plurality of different cell culture solutions on invasion and metastasis, is convenient for sampling cells in matrix gel, is convenient for regular observation, and prevents mutual influence.

Description

technical field [0001] The invention relates to a multi-channel 3D cell invasion and metastasis assay device. Background technique [0002] Tumor invasion refers to the process in which tumor cells infiltrate from their origin along the interstitial space to the surrounding tissue, and its sign is that tumor cells break through the basement membrane. Tumor invasion is the result of the interaction between tumor cells and the surrounding stroma, and is the first step in tumor spread. Tumor metastasis is the process in which malignant tumor cells invade lymphatic vessels, blood vessels or body cavities from the primary site, migrate to new locations and generate new or secondary tumors in the new environment. Different from the planar growth mode (2D) of cell lines, the 3D cell growth state is in a three-dimensional form, including cell microspheres (spheroids) and organoids (organoids). 3D cells grow closer to human cells, so they can better reflect the in vivo state of tum...

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Problems solved by technology

[0005] At present, 2D cultured cells use the Transwell method to detect cell invasion and metastasis; 3D cultured cells have few methods to detect invasion and metastasis. The detection method of Trevigen (Cultrex® 3D Spheroid Cell Invasion Assay) does not have a special detection kit, and It is to directly add extracellular matrix (equivalent to Matrigel) into the 96-well plate, then add fluorescence to the culture medium to mark the cells, observe the morphological changes of the cells through special instruments, and finally need detection software to calculate the results; this detection The design of the method is relatively simple, and it needs to add fluorescence to label the cells, which may cause damage to the cells and affect the experimental results, and this method requires special instruments to track the fluorescently labeled cells, which increases the cost of detection; in addition, this method is currently not available Detecting cell-to-cell interactions

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