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Preparation method of anti-falling glass slide

A glass slide and detachment-preventing technology, which is applied in the preparation, sampling, and measuring devices of test samples, can solve the problems of sample detachment, and achieve the effect of improving adsorption efficiency, reducing sample detachment rate, and firm adsorption

Active Publication Date: 2020-11-06
ZHUHAI LITUO DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of anti-shedding glass slides, to solve the problem of sample stripping in the process of histoimmunology experiments, and to improve the adsorption efficiency and adsorption stability of cells in the process of cell immunology experiments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1: Processing slides

[0025] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0026] 2: glue

[0027] A: The anti-degumming solution is prepared by dissolving 0.2g chitin in 3ml water. Then add 180ml absolute ethanol.

[0028] B: The glass slide is facing upwards and placed horizontally on the plexiglass plate. Check the front and back of each plexi...

Embodiment 2

[0032] 1: Processing slides

[0033] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0034] 2: glue

[0035] A: The anti-degumming solution is prepared by dissolving 0.1g chitin in 3ml water. Then add 180ml absolute ethanol.

[0036] B: The glass slide is facing upwards and placed horizontally on the plexiglass plate. Check the front and back of each plexi...

Embodiment 3

[0040] 1: Processing slides

[0041] Heat the purified water to 70°C, add the laundry powder into the hot water according to the mass ratio of washing powder (Liby non-phosphorus washing powder): purified water: 1:500, stir until completely dissolved, dissolve and let stand. Allow slides to be completely submerged in detergent water. Soak for 30 minutes, and keep the water temperature above 50°C throughout the soaking process. After soaking, rinse immediately under running water until there is no foam and no marks left. Dehydrate the cleaned slides in 95% ethanol for 1-2 minutes and take them out. After the alcohol on the glass slide evaporates naturally, put it in an oven and store it for later use.

[0042] 2: glue

[0043] A: Anti-degumming liquid preparation, dissolve 0.3g chitin in 3ml water, then add 180ml absolute ethanol.

[0044] B: The glass slide is facing upwards and placed horizontally on the plexiglass plate. Check the front and back of each plexiglass plate...

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PUM

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Abstract

The invention discloses a preparation method of an anti-falling glass slide, and belongs to the technical field of glass slides. The method comprises the following steps in sequence: S1, treating a slide, adding washing powder into hot water according to a mass ratio of the washing powder to purified water of 1: 500, stirring until the washing powder is completely dissolved, and preparing a cleaning solution; soaking the glass slide in washing powder water; after the cleaning is completed, storing in a drying oven for later use; S2, preparing an anti-degumming liquid: weighing a certain amountof chitin, and dissolving the chitin in water with a corresponding volume; adding absolute ethyl alcohol with a corresponding volume, and fully and uniformly stirring to fully dissolve floccule-freesubstances; wherein the front surface of the glass slide is upward, horizontally placing the glass slide on an organic glass plate, sucking an anti-degumming liquid by using a pipettor, and dripping the anti-degumming liquid in a circular area of the glass slide, so that the anti-degumming liquid is naturally unfolded on the glass slide; and S3, drying: putting the slide coated with the glue solution into a constant-temperature drying box for drying. The adsorption efficiency of the sample and the stability of the adsorbed sample can be improved.

Description

technical field [0001] The invention belongs to the technical field of glass slides, and in particular relates to a preparation method of an anti-shedding glass slide. Background technique [0002] In recent years, immunohistochemistry has been widely used in in vitro detection. When making plant slices and tissue slices, paraffin sections need to use adhesives. fall off. Protein paste, gelatin paste, and collodion paste can be used for conventional section staining, but in some special histochemical staining, such as immunohistochemistry, it often cannot withstand repeated shaking washing, trypsin digestion, etc., resulting in desquamation. film, impact observation and result analysis. In order to ensure the normal progress of the immunohistochemical experiment, it is required to properly treat the slides before the patch, and the adhesive must be treated on the cleaned slides to prevent the slides from falling off. Therefore in order to solve this problem, we have desig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/44
CPCG01N1/2813G01N1/28G01N1/30G01N1/44
Inventor 雍学安
Owner ZHUHAI LITUO DEV