A process for obtaining total lipids of eicosapentaenoic acid in phospholipid form
An eicosapentaenoic acid and form technology, which is applied in chemical instruments and methods, fat oil/fat production, edible phospholipid compositions, etc., can solve the problems of low total fat yield, sharp drop in krill yield, and lack of fishery resources. , to achieve high yield and wide-ranging effects
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Embodiment 1
[0026] Embodiments of the present disclosure provide a method for obtaining total lipids containing eicosapentaenoic acid in the form of phospholipids, the method comprising:
[0027] The earthworms are cleaned and dried to obtain earthworm powder; the earthworms are ringworms collected in vegetable fields.
[0028] Total lipids in earthworm meal containing eicosapentaenoic acid in the form of phospholipids were extracted.
[0029] Specifically, the drying method is freeze drying.
[0030] Further, the freeze-drying method is liquid nitrogen freeze-drying.
[0031] Specifically, the method for extracting eicosapentaenoic acid in the form of phospholipids in earthworm powder comprises: weighing 100 mg of earthworm powder and putting it into a mortar, adding 0.9 mL of acetic acid solution with a concentration of 0.15 M to the earthworm powder and grinding to obtain Mixture, add 1.25mL of chloroform and 1.25mL of methanol mixture and 1mL of water to the mixture, mix well and th...
Embodiment 2
[0035] Embodiments of the present disclosure provide a method for obtaining total lipids containing eicosapentaenoic acid in the form of phospholipids, the method comprising:
[0036] The earthworms are cleaned and dried to obtain earthworm powder; the earthworms are ringworms collected in vegetable fields.
[0037] Total lipids in earthworm meal containing eicosapentaenoic acid in the form of phospholipids were extracted.
[0038] Specifically, the drying method is freeze drying.
[0039] Further, the freeze-drying method is liquid nitrogen freeze-drying.
[0040] Specifically, the method for extracting eicosapentaenoic acid in the form of phospholipids in earthworm powder comprises: weighing 100 mg of earthworm powder and putting it into a mortar, adding 0.9 mL of acetic acid solution with a concentration of 0.15 M to the earthworm powder and grinding to obtain Mixture, add 1.25mL of chloroform and 1.25mL of methanol mixture and 1mL of water to the mixture, mix well and th...
Embodiment 3
[0044] Embodiments of the present disclosure provide a method for obtaining total lipids containing eicosapentaenoic acid in the form of phospholipids, the method comprising:
[0045] The earthworms are cleaned and dried to obtain earthworm powder; the earthworms are ringworms collected in vegetable fields.
[0046] Total lipids in earthworm meal containing eicosapentaenoic acid in the form of phospholipids were extracted.
[0047] Specifically, the drying method is freeze drying.
[0048] Further, the freeze-drying method is liquid nitrogen freeze-drying.
[0049] Specifically, the method for extracting eicosapentaenoic acid in the form of phospholipids in earthworm powder comprises: weighing 100 mg of earthworm powder and putting it into a mortar, adding 0.9 mL of acetic acid solution with a concentration of 0.15 M to the earthworm powder and grinding to obtain Mixture, add 1.25mL of chloroform and 1.25mL of methanol mixture and 1mL of water to the mixture, mix well and th...
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