Concentration and preservation method of mumps attenuated live vaccine viruses

A technology for live attenuated vaccine and mumps, which is applied in the field of vaccine viruses and can solve the problems of deviation of virus concentration, time-consuming and laborious, and complicated storage process of virus liquid.

Active Publication Date: 2020-11-10
天津中逸安健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are deviations in the virus concentration in the virus harvesting liquid between batches, and the consistency between each batch of products cannot be well controlled; in the actual production process, the amount of each batch of virus harvesting liquid is large, and the preservation process of the virus liquid is cumbersome, time-consuming and laborious. Increased cost, high virus titer loss, and there is no best way to solve this problem

Method used

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  • Concentration and preservation method of mumps attenuated live vaccine viruses
  • Concentration and preservation method of mumps attenuated live vaccine viruses
  • Concentration and preservation method of mumps attenuated live vaccine viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Concentrated preservation and stability detection of live attenuated mumps vaccine virus

[0042] 1) Preparation of cell suspension: Dissect the SPF eggs hatched for 9-11 days, discard the chicken embryo head and viscera, and cut the tissue block into about 2-3mm with scissors 3 For small pieces, use 0.75‰ trypsin solution to digest the tissue pieces, discard the digestion solution after digestion for 20 minutes, use a blowing tube to blow the tissue pieces until they are paste, then add cell nutrient solution, shake well and let stand, until the tissue pieces are fully After sinking, take out the upper cell nutrient solution, which is the cell suspension;

[0043] 2) Preparation of monolayer cells: Dilute the cell suspension with cell nutrient solution to a cell concentration of 2×10 5 cells / ml, distributed in 10-layer cell factories, adding 200ml of liquid to each layer of cell factories, and cultured in a constant temperature room at 36.5±0.5°C until the cells grow ...

Embodiment 2

[0063] Concentrated preservation and stability detection of live attenuated mumps vaccine virus

[0064] 1) Preparation of cell suspension: Dissect the SPF eggs hatched for 9-11 days, discard the chicken embryo head and viscera, and cut the tissue block into about 2-3mm with scissors 3 For small pieces, use 0.75‰ trypsin solution to digest the tissue pieces, discard the digestion solution after digestion for 20 minutes, use a blowing tube to blow the tissue pieces until they are paste, then add cell nutrient solution, shake well and let stand, until the tissue pieces are fully After sinking, take out the upper cell nutrient solution, which is the cell suspension;

[0065] 2) Preparation of monolayer cells: Dilute the cell suspension with cell nutrient solution to a cell concentration of 2×10 5 cells / ml, distributed in 10-layer cell factories, adding 200ml of liquid to each layer of cell factories, and cultured in a constant temperature room at 36.5±0.5°C until the cells grow ...

Embodiment 3

[0085] Concentrated preservation and stability detection of live attenuated mumps vaccine virus

[0086] 1) Preparation of cell suspension: dissect the SPF eggs hatched for 9-11 days, discard the chicken embryo head and viscera, and cut the tissue block into about 2-3mm with scissors 3 For small pieces, use 0.75‰ trypsin solution to digest the tissue pieces, discard the digestion solution after digestion for 20 minutes, use a blowing tube to blow the tissue pieces until they are paste, then add cell nutrient solution, shake well and let stand, until the tissue pieces are fully After sinking, take out the upper cell nutrient solution, which is the cell suspension;

[0087] 2) Preparation of monolayer cells: Dilute the cell suspension with cell nutrient solution to a cell concentration of 2×10 5 cells / ml, distributed in 10-layer cell factories, adding 200ml of liquid to each layer of cell factories, and cultured in a constant temperature room at 36.5±0.5°C until the cells grow ...

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Abstract

The invention discloses a concentration and preservation method of mumps attenuated live vaccine viruses. The method comprises the following steps of preparing a cell suspension, preparing monolayer cells, preparing a virus solution, carrying out ultrafiltration concentration, preparing a stock solution, and carrying out cryopreservation to obtain a mumps attenuated live vaccine virus concentratedsolution with extremely low virus titer loss rate, and then a semi-finished product preparation and freeze-drying method is combined, and therefore, it can be guaranteed that the virus titer of the mumps attenuated live vaccine virus vaccine is almost not lost in the long-time storage process.

Description

technical field [0001] The invention belongs to the technical field of vaccine viruses, and in particular relates to a method for concentrating and preserving live attenuated mumps vaccine viruses. Background technique [0002] Live attenuated vaccine refers to a type of vaccine that, after the pathogen is treated, the structure of the A subunit (toxic subunit) changes and the toxicity is weakened, but the activity of the B subunit (binding subunit) remains unchanged, that is, a type of vaccine that maintains antigenicity ; Inoculating it into the body will not cause disease, but the pathogen can trigger the body's immune response, stimulate the body to produce specific memory B cells and memory T cells, and play a role in obtaining long-term or lifelong protection. Compared with vaccines (killed vaccines), this type of vaccine has stronger immunity and a longer duration of action. [0003] At present, in the field of live attenuated mumps vaccines, after the virus liquid i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02A61K39/165A61P31/14C12R1/93
CPCC12N7/00A61K39/12A61P31/14C12N2760/18751C12N2760/18734A61K2039/5254
Inventor 杨玉国高辉谈小荣王小宁李雪峰兰明明林新旭
Owner 天津中逸安健生物科技有限公司
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