Method for creating weakly photosensitive indica rice restore line

A technology for restorer lines and indica rice is applied in the field of creating weakly sensitive indica rice restorer lines, which can solve the problem of not many rice varieties and achieve the effect of improving breeding efficiency.

Pending Publication Date: 2020-11-20
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most high-yield rice varieties in production have obvious geographical limitations, but they have wide adaptability, that is, they can make full use of local heat resources in areas with a large latitude range, and there are not many high-yield rice varieties.

Method used

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  • Method for creating weakly photosensitive indica rice restore line
  • Method for creating weakly photosensitive indica rice restore line
  • Method for creating weakly photosensitive indica rice restore line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Selection of Parents Shan 71 and Zhonghui 161

[0036] 1. Rice material

[0037] Both Dan 71 and Zhonghui 161 are two indica restorer lines independently bred by the China Rice Research Institute. Among them, Dan 71 is a restorer line of three-line indica hybrid rice, and Zhonghui 161 is a restorer line of three-line late indica hybrid rice.

[0038] 2. Design of sequencing primers for Hd1, Ghd7, Ghd8 and Hd2

[0039] According to reports, the Hd1, Ghd8 and Hd2 alleles of Nipponbare and the Ghd7 allele of Minghui 63 all have functions, so download the Nipponbare alleles of Hd1, Ghd8 and Hd2 from the database (rice.plantbiology.msu.edu) respectively Gene genome sequence and coding region (cDNA sequence) (LOC_Os06g16370, LOC_Os08g07740 and LOC_Os07g49460), downloaded from the database (www.ncbi.nlm.nih.gov) to obtain the Minghui 63 allele genome sequence (EU286801) and cDNA sequence of Ghd7 (EU286800).

[0040] Oligo 7.0 software was used to design sequencing...

Embodiment 2

[0066] Example 2 Screening of Restorer Lines of Indica Rice with Weak Light Sensitivity Among Dan 71 / Zhonghui 161 Recombinant Inbred Lines

[0067] 1. Rice material

[0068] With Shan 71 as the female parent and Zhonghui 161 as the male parent, a group of recombinant inbred lines was developed, including 260 F7 lines, by continuous selfing after hybridization. In the summer of 2019, the set of recombinant inbred lines was planted in the experimental base of the China Rice Research Institute in Hangzhou, Zhejiang Province by staged sowing. The sowing dates for the three phases are: April 28, May 15 and June 5.

[0069] 2. Development of Ghd8 and Hd2 gene markers

[0070] For the 9-bp and 8-bp insertion / deletion variations on the cDNA sequences of Ghd8 and Hd2 genes, PCR primers were designed using Oligo7.0 software to detect the genotype and gene marker information of each strain in the recombinant inbred population Listed in Table 2.

[0071] Table 2 The present invention ...

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Abstract

The invention discloses a method for creating a weakly photosensitive indica rice restore line. The method comprises the following steps: firstly, selecting 2 indica rice restore systems having rice heading period genes Hd1 being a loss-of-function type, Ghd7 being function type and Ghd8 and Hd2 being function type as parents; performing continuous selfing after hybridization, and acquiring a recombinant selfing line population by utilizing a single seed descent method; comparing heading periods of three sowing periods of strains with different genotype combinations by adopting a mode of sowing at different stages to obtain a weakly photosensitive indica rice restore line carrying loss-of-function type Hd1 and function type Ghd7, Ghd8 and Hd2, namely hd1Ghd7Ghd8Hd2 genotype combined weaklyphotosensitive indica rice restore line. The method combines conventional breeding and molecular technique, can improve the breeding efficiency against specific gene screening, and provides theoretical basis and an intermediate material to breeding of indifferent rice varieties in southern rice regions.

Description

technical field [0001] The invention relates to the technical field of rice molecular breeding, in particular to a method for creating a restorer line of weakly light-sensitive indica rice. Background technique [0002] The heading date of rice determines the adaptability of rice varieties to different cultivation areas and cultivation seasons, and is an important agronomic trait in rice production. The southern rice region is an important rice production area in my country, especially indica rice, with a large regional span and diverse ecological conditions. At present, most high-yield rice varieties have obvious geographical limitations, but they have wide adaptability, that is, they can make full use of local heat resources in areas with a large latitude range, and there are not many high-yield rice varieties. Breeding rice varieties with wide adaptability has gradually become one of the important goals of rice breeding. [0003] Hd1, Ghd7, Ghd8, and Hd2 are the four ke...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor樊叶杨庄杰云张振华朱玉君
OwnerCHINA NAT RICE RES INST