Unlock instant, AI-driven research and patent intelligence for your innovation.

Ovalbumin zymolyte as well as preparation method and application thereof

A technology for proteolysis and ovalbumin, which is applied to ovalbumin, albumin peptides, hydrolyzed protein components, etc., can solve the problem that the function of the enzymatic hydrolysis products is not ideal, and achieve synergistic immune enhancement, immunity enhancement, and good effects. Effect

Active Publication Date: 2020-11-20
杭州佰倍优生物科技有限公司
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many methods in the prior art to enzymatically hydrolyze ovalbumin, but the function of the obtained enzymatic hydrolyzate is not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ovalbumin zymolyte as well as preparation method and application thereof
  • Ovalbumin zymolyte as well as preparation method and application thereof
  • Ovalbumin zymolyte as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment provides a kind of preparation method of ovalbumin hydrolyzate, and described preparation method comprises the following steps:

[0023] (1) Dissolving 1kg ovalbumin powder in 2.5kg distilled water to obtain ovalbumin solution;

[0024] (2) adjust the pH value of the ovalbumin solution obtained in step (1) to 8.5, add 1.5g composite protease, and enzymolyze it for 8 hours at 50° C. under agitation to obtain an enzymolysis solution; the composite protease is a mass ratio of 2:2:1 mixture of papain, trypsin and Bacillus pumilus protease;

[0025] (3) inactivating the enzymolysis solution obtained in step (2) at 90-100° C. for 10 minutes;

[0026] (4) Centrifuge the enzymolyzed solution at 5000r / min for 10 minutes, collect the supernatant, sterilize the supernatant at 121°C for 20 minutes, then concentrate under reduced pressure and freeze-dry to obtain a dry powder , which is ovalbumin hydrolyzate.

Embodiment 2

[0028] This embodiment provides a kind of preparation method of ovalbumin hydrolyzate, and described preparation method comprises the following steps:

[0029] (1) Dissolving 1kg ovalbumin powder in 2.5kg distilled water to obtain ovalbumin solution;

[0030] (2) adjust the pH value of the ovalbumin solution obtained in step (1) to 7, add 1.5g composite protease, and enzymolyze it for 8 hours at 50° C. under agitation to obtain an enzymolysis solution; the composite protease is a mass ratio of 2:2:1 mixture of papain, trypsin and Bacillus pumilus protease;

[0031] (3) inactivating the enzymolysis solution obtained in step (2) at 90-100° C. for 10 minutes;

[0032] (4) Centrifuge the enzymolyzed solution at 5000r / min for 10 minutes, collect the supernatant, sterilize the supernatant at 121°C for 20 minutes, then concentrate under reduced pressure and freeze-dry to obtain a dry powder , which is ovalbumin hydrolyzate.

Embodiment 3

[0034] This embodiment provides a kind of preparation method of ovalbumin hydrolyzate, and described preparation method comprises the following steps:

[0035] (1) Dissolving 1kg ovalbumin powder in 2.5kg distilled water to obtain ovalbumin solution;

[0036] (2) adjust the pH value of the ovalbumin solution obtained in step (1) to 10, add 1.5g composite protease, and enzymolyze it for 8 hours at 50° C. under agitation to obtain an enzymolysis solution; the composite protease is a mass ratio of 2:2:1 mixture of papain, trypsin and Bacillus pumilus protease;

[0037] (3) inactivating the enzymolysis solution obtained in step (2) at 90-100° C. for 10 minutes;

[0038] (4) Centrifuge the enzymolyzed solution at 5000r / min for 10 minutes, collect the supernatant, sterilize the supernatant at 121°C for 20 minutes, then concentrate under reduced pressure and freeze-dry to obtain a dry powder , which is ovalbumin hydrolyzate.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an ovalbumin zymolyte as well as a preparation method and application thereof. The ovalbumin zymolyte is prepared by performing combined enzymolysis on three enzymes including papain, trypsin and bacillus pumilus protease. The ovalbumin zymolyte has a relatively good effect in improving liver functions. The ovalbumin zymolyte is compounded with a ganoderma lucidum extract, so that the immunity can be enhanced through synergistic interaction.

Description

technical field [0001] The invention specifically relates to an ovalbumin hydrolyzate and its preparation method and application. Background technique [0002] Protein that is part of albumin in egg white. Ovalbumin is usually referred to as its main component (about 65% of egg white). In addition to this there is also a small amount (about 14%) of an albumin called conalbumin present in the egg white. However, ovalbumin is not easy to be directly absorbed by the body and skin when it is applied. It must be hydrolyzed into amino acids and polypeptides before it can be absorbed by the body. As a biologically active peptide, enzymatically hydrolyzed ovalbumin polypeptide has physiological functions such as anti-mutation, anti-radiation, anti-fatigue, liver protection, anti-oxidation, lowering blood fat, and lowering blood pressure. development and application value. There are many methods in the prior art to enzymatically hydrolyze ovalbumin, but the functions of the obtai...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/77C12P21/06A61K38/01A61P37/04A61P1/16A61K36/074
CPCA61K36/074A61K38/014A61P1/16A61P37/04C07K14/77C12P21/06A61K2300/00
Inventor 余钧
Owner 杭州佰倍优生物科技有限公司