Isaria javanica strain and application thereof in prevention and treatment of phyllotreta striolata

A coryneform spore and strain technology, which is applied in the field of biological pesticides, can solve the problems such as no document discloses Corynebacterium javanica and the like, and achieves the effects of high efficiency, environmental and human safety in preventing and controlling flea beetle.

Active Publication Date: 2020-11-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there is no literature to disclose the re

Method used

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  • Isaria javanica strain and application thereof in prevention and treatment of phyllotreta striolata
  • Isaria javanica strain and application thereof in prevention and treatment of phyllotreta striolata
  • Isaria javanica strain and application thereof in prevention and treatment of phyllotreta striolata

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0029] This example is the determination of the pathogenicity of different entomogenous fungal strains to the adult flea beetle, and the strain IjH6102 with the highest activity is screened out.

[0030] By biological activity assay (method: dipping method; number of treated worms: 30 adults), it was found that among the more than 300 strains of entomogenous fungi that were tested on behalf of others, the activity of the IjH6102 strain IjH6102 on the flea beetle The highest, when the treatment concentration is 1.0×10 8 When 1 spore / mL, its lethality to the flea beetle adult reaches 60.89%, and the pathogenicity of different bacterial strains to the flea beetle adult is as shown in table 1 (the bacterial strain that the corrected mortality rate is less than 10% is not listed out).

[0031] Table 1 The pathogenicity of different strains to the adult flea beetle

[0032]

[0033]

Embodiment 2

[0035] This embodiment is the morphological characteristics of the IjH6102 bacterial strain of I. javanica

[0036] The morphological characteristics of the strain are as follows figure 1 As shown, on the PDA plate, the initial stage of the colony is white, and the surface is fluffy. After being cultivated for 7 days, the surface of the bacterial strain has an apricot powdery spore layer, no bundle of spores, and the back of the colony is light apricot. Under the optical microscope, the hyphae are colorless and transparent, with a width of 2.68±0.21 μm; the conidiophores are similar to the hyphae and do not form spore bundles; the phialides (sporulation cells) are gourd-shaped, with a diameter of 2.85±0.29 μm at the widest point, 7.98±0.81μm long, wheel-shaped directly attached to the mycelia or conidiophores; phialides; conidia colorless, oblong, pointed at both ends, 5.38±0.63μm long, 2.12±0.25 wide μm.

Embodiment 3

[0038] This embodiment is the molecular biology identification of the IjH6102 bacterial strain of I. javanica

[0039] The present invention has carried out molecular biology identification on this bacterial strain, and its ITS1-5.8S-ITS4 rDNA gene-specific amplified product 536bp is shown in SEQ ID NO: 1, and the phylogenetic tree constructed using MEGA software shows that it is similar to I. bacteria Isariajavanica in the same species (eg figure 2 shown). Combined with the characteristics of morphology and molecular biology, it is clear that the taxonomic status of the strain belongs to Ascomycota, Sordariomycetes, Hypocreales, Cordycipitaceae, and Isosporium Isaria javanica of (Isaria).

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Abstract

The invention relates to an isaria javanica IjH6102 strain and also relates to application of the isaria javanica strain in prevention and treatment of phyllotreta striolata, and belongs to the technical field of biological pesticides. The isaria javanica IjH6102 strain belongs to fungi, Ascomycota, Sordariomycetes, Hypocreales, Cordycipitaceae and Isaria according to the taxonomic status, the strain is collected in Guangdong Microbial Culture Collection Center on December 18, 2019, with the collection number of GDMCC No: 60936. The strain is an entomopathogenic fungus and a biological livingpesticide, is highly effective in preventing and treating the phyllotreta striolata, is safe to the environment and the human body, and has the potential as an environment-friendly fungal insecticide.

Description

technical field [0001] The invention relates to a strain of I. javanica, and also relates to the application of the strain of I. japonicus in preventing and treating flea beetle, belonging to the technical field of biopesticides. Background technique [0002] Phyllotreta striolata (Fabricius) belongs to the Coleoptera (Coleoptera) Chrysomelidae pests. In recent years, the planting scale of cruciferous vegetables has expanded year by year, and the yellow flea beetle has also risen as a major pest, seriously affecting Chinese cabbage, Production of cruciferous vegetables such as radishes. The living habits of the flea beetle are complex. Both adults and larvae can damage crops. The adults have strong migration ability and can eat up the whole seedlings, and can also cause holes in the leaves to affect the appearance; the larvae live in the soil and take Feeds on the roots of plants, and when the damage is serious, it can cause the death of the whole plant; at the same time, a...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 胡琼波翁群芳袁文静张晓锋
Owner SOUTH CHINA AGRI UNIV
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