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A method for improving the drought resistance of apples by external application of artificial synthetic peptides

A synthetic, drought-resistant technology, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve problems such as uncertainty, reduce excess accumulation, increase photosynthetic rate and antioxidant enzymes Activity, the effect of improving the drought resistance of apples

Active Publication Date: 2021-11-12
NORTHWEST A & F UNIV
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Problems solved by technology

Previous studies have studied the effects of overexpression of the MdEPF2 gene in tomato on stomatal density and the changes in WUE and physiological indicators of transgenic plants under long-term drought. There is no report on the method of improving the drought resistance of plants

Method used

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  • A method for improving the drought resistance of apples by external application of artificial synthetic peptides
  • A method for improving the drought resistance of apples by external application of artificial synthetic peptides
  • A method for improving the drought resistance of apples by external application of artificial synthetic peptides

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Embodiment Construction

[0041] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0042] 1 Materials and methods

[0043] 1.1 Plant material

[0044] Pingyi sweet tea seedlings were used as materials for exogenous polypeptide treatment.

[0045] 1.2 Main reagents and instruments

[0046] Main chemical reagents: plant RNA extraction kit was purchased from Tiangen Company, cDNA first-strand synthesis reverse transcription kit was purchased from Takara, Premix Ex Taq TM II kit Takara, T4-DNA ligase was purchased from Thermofisher. MS medium, mannitol, rifampicin, streptomycin sulfate and kanamycin were purchased from MP. Diaminobenzidine (DAB) and nitroblue tetrazole (NBT) were purchased from Solarbio. Other conventional reagents were Sigma aliquots or domestically produced.

[0047] Main instruments: desktop low-temperature high-speed centrifuge (Eppendorf Centrifuge 5810R), electro...

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Abstract

The invention discloses a method for improving the drought resistance of apples by applying artificially synthesized peptides, which comprises the following steps: step 1, artificial synthesis of mEPF2 polypeptide; step 2, dialysis refolding and HPLC detection of the synthetic peptide; step 3, refolding Determination of peptide concentration; step 4, preparation of polypeptide treatment solution and treatment of apple plants; step 5, comparison of phenotypes of treated apple plants and measurement and analysis of physiological indicators. The invention treats apple leaves with the artificially synthesized mEPF2 exogenous polypeptide, which can adjust the stomatal density of the leaves under drought, reduce the water loss rate, increase the photosynthetic rate and antioxidant enzyme activity, and reduce the excessive accumulation of peroxides, thereby improving the drought tolerance of apple plants capacity and water use efficiency under drought conditions. The results of the study are of great significance for further understanding the role of MdEPF2 in apple development and response to drought stress, and improving apple drought resistance through exogenous spraying of artificial peptides.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, and relates to a method for improving the drought resistance of apples by applying artificially synthesized peptides, in particular to a method for improving the drought resistance of apples by applying artificially synthesized MdEPF2 peptides. Background technique [0002] Plants need adequate water for growth, development and reproduction. When the drought is severe, it can lead to the death of large areas of vegetation and the deterioration of the climate, resulting in a large area of ​​crop production reduction or even failure, resulting in huge social problems and economic losses. [0003] Drought has adverse effects on many aspects of plant growth and reproduction, including affecting seed germination rate and seedling growth. During the vegetative growth stage, drought stress will seriously limit plant growth and development. Under drought stress, the water potential, relat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N47/44A01P21/00A01G17/00A01G7/06
CPCA01G7/06A01G17/005A01N47/44
Inventor 马锋旺毛柯蒋琦李超刘长海龚小庆
Owner NORTHWEST A & F UNIV
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