Electrochemical luminescence immunoassay method and kit

A technique of luminescence immunoassay and assay method, applied in the field of immunology, can solve the problems of complicated operation steps, high cost, low sensitivity, etc., and achieve the effect of avoiding low sensitivity and highlighting substantive characteristics.

Inactive Publication Date: 2020-11-24
郑州迪安医学检验所有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods usually have defects such as low sensitivity, high cost, and cumbersome operation steps.

Method used

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  • Electrochemical luminescence immunoassay method and kit
  • Electrochemical luminescence immunoassay method and kit
  • Electrochemical luminescence immunoassay method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] This embodiment specifically provides an electrochemiluminescence immunoassay method, and the specific steps include:

[0023] First incubation: 20 μL of sample, biotinylated cortisol-specific antibody, and ruthenium pyridinium-labeled cortisol derivative are incubated with the labeled antibody binding site partially occupied by the sample analyte and partially occupied by the ruthenium-labeled hapten , depending on the concentration of the analyte in the sample and the formation of the respective immune complexes, wherein the pyridine ruthenium-labeled cortisol derivative is 4-dipropylaminobutyl-4'-methyl-2,2'-linked pyridine.

[0024] The second incubation: adding streptavidin-coated magnetic bead particles for incubation, through the interaction between biotin and streptavidin, the complex is bound to the magnetic bead particles to obtain the antigen-antibody magnetic particle complex;

[0025] The antigen-antibody magnetic particle complex is sucked into the measur...

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PUM

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Abstract

The invention provides an electrochemical luminescence immunoassay method which comprises the following specific steps: fully reacting a sample, a biotinylated cortisol specific antibody and a ruthenium pyridine labeled cortisol derivative, and then adding streptavidin-coated magnetic bead particles for incubation to obtain an antigen-antibody magnetic particle complex; sucking the antigen-antibody magnetic particle complex into a measuring cell, adding tripropylamine, applying voltage to generate ECL reaction, enabling the antigen-antibody magnetic particle complex to emit chemiluminiscence,and measuring luminous intensity through a photomultiplier so as to obtain the concentration of cortisol. The method is convenient and rapid, and accurate full automation is achieved. The invention also provides a kit which can realize recycling of the marker and is long in luminescence time, high in intensity and easy to determine, so that the detection sensitivity is improved, the linear range is widened, and the dilution error of a high-value specimen is reduced.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to an electrochemiluminescence immunoassay method and a kit. Background technique [0002] Cortisol (hydrocortisone) is the most important glucocorticoid in the body and plays an important role in maintaining multiple physiological functions of the body. Like other glucocorticoids, cortisol is synthesized in the zona fascicularis of the adrenal cortex from common cholesterol precursors. Approximately 90% of circulating cortisol is transported bound to corticosteroid-binding globulin (CBG) and albumin. Only a small amount of free cortisol reacts with its receptors. [0003] The concentration of serum cortisol has a circadian rhythm. Usually the highest peak appears in the early morning (700nmol / L or 25.4mg / dL), then gradually decreases, and can be reduced to about half of the peak concentration at night. It is therefore important to specify when the blood was drawn when interp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/543G01N33/532G01N33/58G01N21/76
CPCG01N21/76G01N33/532G01N33/54326G01N33/583G01N33/74
Inventor 陈秀马敬柳新新齐振普
Owner 郑州迪安医学检验所有限公司
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