Method for making ranunculus japonicus regeneration system

A ranunculus, system technology, applied in the field of plant tissue culture and rapid propagation, can solve problems such as the inability to establish a genetic transformation system and the inability to achieve genetic improvement at the level of molecular biology, and achieve accelerated reproduction, large numbers, and fast bud growth Effect

Pending Publication Date: 2020-11-27
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Since no callus is produced during the sowing process, a genetic transformation system cannot be established, and genetic improvement at the level of molecular biology cannot be achieved.

Method used

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  • Method for making ranunculus japonicus regeneration system
  • Method for making ranunculus japonicus regeneration system
  • Method for making ranunculus japonicus regeneration system

Examples

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Embodiment 1

[0045] Cultivation of Septic Balloons of Ranunculus 'Red Years'

[0046] (1) Preparation of explants Select healthy Ranunculus 'Red Age' tubers with a diameter of about 3.0 cm, soak them in 2 g / L carbendazim aqueous solution for 15 minutes, and then bury them shallowly in a moist fine sand substrate treated by autoclaving In the light of 350μmol·m -2 ·s -1 , 12h / d, and (21±1)°C for pre-cultivation to germinate the bulbs. After 8 days, select bulbs with a bud length of about 1 cm, and inoculate them after surface disinfection. The specific disinfection method is: scrub the tubers with a soft brush to avoid damage to the terminal buds, cut off the tuber skin, shake and wash in detergent solution for 21 minutes, soak in 2g / L carbendazim solution for 1.0 hours, and rinse with tap water for 30 minutes. Transfer to the ultra-clean bench, cut off the surface of the tuber, keep the tuber with a diameter of about 1.5cm, soak it in 75% alcohol for 45s, rinse it once with sterile water...

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Abstract

The invention provides a culture medium for inducing ranunculus japonicus to generate callus and application of the culture medium in establishing a ranunculus japonicus regeneration system, and relates to the technical field of plant tissue culture and rapid propagation. The culture medium takes an MS culture medium as a basic culture medium and further comprises 30g / L of saccharose, 7g / L of agar, 2.4-2.8mg / L of 6-BA, 0.5-1.5mg / L of NAA, and 0.5-1.5mg / L of carbon nano tubes. The culture medium can induce ranunculus japonicus to generate callus, multiple shoots are induced from the callus, theranunculus japonicus regeneration system is established, and about three months are needed; the number of the multiple shoots induced from the callus is large, the shoot growth speed is high, the propagation coefficient of the shoots can be greatly increased, meanwhile, the propagation speed of the shoots is increased, and the cost of ranunculus japonicus tissue culture seedlings is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and rapid propagation, and in particular relates to a medium for inducing a ranunculus to produce callus and its application in establishing a ranunculus regeneration system. Background technique [0002] Ranunculus asiaticus L., also known as grass peony, Persian buttercup, foreign peony. Ranunculaceae, Ranunculus perennial herbaceous flowers. Native to Turkey, Syria, Iran, and southeastern Europe. Ranunculus has bright and rich flower colors, including red, white, orange, pink, etc., and has high ornamental value. It has been widely planted in Europe and the United States, and is now cultivated in all countries in the world. my country began to introduce and plant it as early as the 1980s. In New Zealand, the Netherlands, Japan, the United States, Israel and other countries, a complete production and reproduction technology system has been established. There are also more and mor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 顾永华陈梅香郑玉红宋正达
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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