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Systemic pesticide detection method applied to fruits and vegetables

A detection method and systemic technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome pre-treatment methods, cannot meet the needs of real-time detection of systemic pesticides, cannot achieve rapid and on-site detection, and achieve The effect of simplifying the time of pre-processing

Pending Publication Date: 2020-12-04
河南诺玛科技有限公司
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Problems solved by technology

[0003] At present, the commonly used detection methods for systemic pesticide residues are mainly large-scale instrument detection methods based on chromatography. This type of method has high requirements for the purification rate of samples and the purification rate of substances to be tested. Therefore, the sample The pretreatment method is relatively cumbersome, unable to meet the requirements of rapid and on-site detection, and cannot meet the needs of real-time detection of systemic pesticides

Method used

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  • Systemic pesticide detection method applied to fruits and vegetables

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Effect test

Embodiment 1

[0025] A method for detecting systemic pesticides applied to fruits and vegetables, comprising the steps of:

[0026] Step 1: Sample preparation, fresh vegetables and fruits were crushed and homogenized for 2 minutes, and set aside;

[0027] Step 2: Weigh 25g of the homogenized fruit and vegetable sample, add 5ml of deionized water, then add acetonitrile to shake and extract, centrifuge and layer at a speed of 2500r / min, and centrifuge for 2min to obtain the supernatant;

[0028] Step 3: Take the supernatant and add 8ml of a mixed solvent of hexane and acetone with a volume ratio of 1:1, shake well to obtain the first mixed solution;

[0029] Step 4: The first mixed solution is eluted with a solid phase extraction column. During the elution process, the mobile phase gradient program is 0-10min, organic phase 15%, 10-21min, organic phase 15-45%, 21 -30min, organic phase 45-85%, 30-35min, organic phase 85-95%, 35-40min, organic phase 95-15%, to obtain the sample solution;

[0...

Embodiment 2

[0032] A method for detecting systemic pesticides applied to fruits and vegetables, comprising the steps of:

[0033] Step 1: Sample preparation, fresh vegetables and fruits were crushed and homogenized for 2-5 minutes, and set aside;

[0034] Step 2: Weigh 27g of the homogenized fruit and vegetable sample, add 6ml of deionized water, then add acetonitrile to shake and extract, centrifuge and layer at a speed of 4000r / min, and centrifuge for 2-8min to obtain the supernatant;

[0035] Step 3: Take the supernatant and add 12ml of a mixed solvent of hexane and acetone with a volume ratio of 1:1, shake well to obtain the first mixed solution;

[0036] Step 4: The first mixed solution is eluted with a solid phase extraction column. During the elution process, the mobile phase gradient program is 0-10min, organic phase 15%, 10-21min, organic phase 15-45%, 21 -30min, organic phase 45-85%, 30-35min, organic phase 85-95%, 35-40min, organic phase 95-15%, to obtain the sample solution; ...

Embodiment 3

[0039] A method for detecting systemic pesticides applied to fruits and vegetables, comprising the steps of:

[0040] Step 1: Sample preparation, fresh vegetables and fruits were crushed and homogenized for 5 minutes, and set aside;

[0041] Step 2: Weigh 30g of the homogenized fruit and vegetable sample, add 8ml of deionized water, then add acetonitrile to shake and extract, centrifuge and layer at a speed of 5000r / min, and centrifuge for 8min to obtain the supernatant;

[0042] Step 3: Take the supernatant and add 16ml of a mixed solvent of hexane and acetone with a volume ratio of 1:1, shake well to obtain the first mixed solution;

[0043] Step 4: The first mixed solution is eluted with a solid phase extraction column. During the elution process, the mobile phase gradient program is 0-10min, organic phase 15%, 10-21min, organic phase 15-45%, 21 -30min, organic phase 45-85%, 30-35min, organic phase 85-95%, 35-40min, organic phase 95-15%, to obtain the sample solution;

[...

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Abstract

The invention provides a systemic pesticide detection method applied to fruits and vegetables. Acetonitrile is adopted for extraction, compared with conventional extraction agents such as normal hexane, acetone and ethyl acetate, the extraction recovery rate of the systemic pesticide is increased by 4%-26%, and the systemic pesticide detection method has obvious advantages especially for the recovery rate of easily-degradable pesticides. A solid-phase extraction column purification mode and the like are adopted, so that the pretreatment time is greatly shortened, and a technical foundation islaid for rapid residue analysis of systemic pesticides. Rapid full scanning and qualitative and quantitative analysis are carried out on multiple systemic pesticide residues in fruits and vegetables,and technical support is provided for high-throughput rapid monitoring of the multiple systemic pesticide residues in the fruits and vegetables. Meanwhile, an effective analysis method is provided forresearching, remaining, degrading and dynamically analyzing the action mechanism of the novel systemic pesticide slow-release agent application method in fruits and vegetables, and technical guidanceis provided for enterprise application for developing the novel systemic pesticide slow-release agent.

Description

technical field [0001] The invention relates to the field of pesticide detection and analysis, in particular to a systemic pesticide detection method applied to fruits and vegetables. Background technique [0002] Systemic pesticides are widely used as a new type of broad-spectrum and high-efficiency pesticides. They are mainly absorbed into plants through plant roots, stems, and leaves, transported in plants to the active parts, and traditional non-systemic pesticides. Compared with insecticides, for the control of cabbage caterpillars, aphids, planthoppers, leafhoppers, etc., the amount of systemic insecticides used is relatively small, and the residual period is longer, which is more effective. However, since systemic pesticides are not affected by environmental factors such as external light and rain erosion in plants, they combine with various substances in fruits and vegetables, especially edible parts, which can lead to continuous accumulation of residues; During cro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 贾明宏王旭梁旭阳贾唯
Owner 河南诺玛科技有限公司
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