Application of miR-132-5p to preparation of nerve regeneration drugs or materials

A technology of nerve regeneration and medicine, applied in the field of biomedicine

Active Publication Date: 2020-12-08
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, there are no studies on the association between miR-132-5p and neurogenesis

Method used

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  • Application of miR-132-5p to preparation of nerve regeneration drugs or materials
  • Application of miR-132-5p to preparation of nerve regeneration drugs or materials
  • Application of miR-132-5p to preparation of nerve regeneration drugs or materials

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Experimental program
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Effect test

Embodiment 1

[0027] 1. Construction of miR-132-5p mimics and inhibitors

[0028] For the construction of miR-132-5p mimics and inhibitors in this experiment, we purchased the micrONTM miRNA series kit from Ruibo Company, provided the mature sequence of miR-132-5p to the company, and synthesized corresponding fragments.

[0029] mir-132-5p inhibitor: 5'-aguaacaaucgaaagccacggu-3' (SEQ ID NO: 2).

[0030] Inhibitor control: 5'-caguacuuuuguguaguacaaa-3' (SEQ ID NO:3).

[0031] Mimic of mir-132-5p: 5'-accguggcuuucgauuguuacu-3' (SEQ ID NO: 4).

[0032] Mock control: 5'-uuuguacuacacaaaaguacug-3' (SEQ ID NO:5).

[0033] 2. Primary dorsal root ganglion (DRG) explant culture and purification and miR-132-5p treatment

[0034] Pregnant 14-15d SD rats were anesthetized with ether in a fume hood to prepare the skin. Sterilized with 75% ethanol, took out the embryonic mouse under aseptic conditions, put it in the dissection solution containing PS, took out the dorsal root ganglion of the embryonic mo...

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Abstract

The invention discloses an application of non-coding RNA (miR-132-5P) to a peripheral nerve regeneration process. The non-coding RNA (miR-132-5P) can promote peripheral extension regeneration by inhibiting the expression of the miR-132-5P. Rat miR-132-5p is knocked down and overexpressed through a genetic engineering technology, and results show that after the miR-132-5p is inhibited, the axon growth speed of neurons is increased, the nerve repair time is shortened, and the nerve function recovery is remarkable.

Description

technical field [0001] The invention belongs to the field of biomedicine. Specifically, it relates to the application of miR-132-5p in the preparation of nerve regeneration drugs or materials. Background technique [0002] Traffic accidents, natural disasters, various industrial injuries and other accidents can cause different types of peripheral nerve damage. According to statistics, nearly 1,000,000 cases of nerve injury are newly added every year in my country, of which about 300,000 cases of neurological deficit. Nerve defects can lead to facial paralysis, drooping wrists and other physical disabilities, causing a serious social burden. [0003] At present, the main treatments are autologous nerve transplantation and allogeneic nerve transplantation. Autologous nerve transplantation (the "gold standard" for the treatment of nerve defects), also known as "remove the wall to fill the wall" cable transplantation, which will lead to the loss of function of the donor site,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7105A61K48/00A61P25/00
CPCA61K31/7105A61P25/00
Inventor 杨宇民孙诚赵亚红梁云云
Owner NANTONG UNIVERSITY
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