A kind of method to promote the accumulation of staurosporine

Abstract

The invention provides a method for promoting the accumulation of staurosporine, and specifically discloses a culture medium for promoting the accumulation of staurosporine, which greatly promotes the accumulation of staurosporine by adopting different vitamin combinations and medium formulas. At the same time, by adding carbon source, the fermentation yield can be increased by more than 40%. The technical scheme provided by the invention is simple and feasible, further improves the production efficiency of the staurosporine, reduces production costs, and facilitates the industrial production and application of the staurosporine.

Description

technical field [0001] The invention relates to the technical field of industrial microbial fermentation, in particular to a method for promoting staurosporine accumulation. Background technique [0002] Staurosporine is an indole alkaloid isolated from Streptomyces staurosporeus in 1977. It has various biological activities such as antibacterial, antifungal, lowering blood pressure, inhibiting platelet aggregation and protecting nerves, especially staurosporine It is also a broad-spectrum kinase inhibitor and has the potential to be developed into an antitumor drug. However, scientists later discovered that its kinase inhibitory activity has poor specificity (it can interact with 90% of the human kinases discovered so far), and the resulting toxic side effects limit the possibility of its becoming a drug. For this reason, pharmacists have synthesized a large number of derivatives, hoping to improve the kinase-specific inhibition of these compounds. Among them, midostaurin...

AI Technical Summary

Problems solved by technology

At present, patents CN108048369A and CN107603922A disclose methods for obtaining staurosporine by fermentation of staurosporine-producing Streptomyces, but the production strains all belong to the genus Marine Streptomyces, seawater needs to be added in the process, and macropores are also required in the process of patent CN107603922A Resin, the highest output is 320mg / L, the actual industrial application is poor

[0004] In order to overcome the shortcomings of the staurosporine fermentation technology, such as unstable strains, low yield, and difficulty in realizing industrial production, our company has bred a brand-new staurosporine-producing strain, which is disclosed in CN108676757A. This strain has the ability to produce It has the advantages of high capacity, high heritage stability, and obvious increase in fermentation yield. However, the disclosed fermentation method still has problems such as insufficient fermentation yield and high fermentation cost, which is not conducive to large-scale industrial production.