Glomerulosclerosis markers for chronic glomerular diseases and detection kits of glomerulosclerosis markers

A technology for glomerular diseases and glomerulosclerosis, applied in the medical field, can solve problems such as unclear pathogenesis, complicated etiology, and high biological markers

Pending Publication Date: 2020-12-08
朱永俊
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Focal segmental glomerulosclerosis (FSGS) is one of the common causes of nephrotic syndrome (NS) in adults. Due to its complex etiology and unclear pathogenesis, clinical diagnosis and treatment of FSGS face many difficulties. Interstitial injury is a common feature of various acute and chronic kidney diseases. Currently, urine biomarkers for clinical diagnosis of tubulointe...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] The embodiment of the present invention provides a chronic glomerular disease glomerulosclerosis marker, the marker is miRNA-196a, miRNA-155, miRNA-490 and interleukin in urine.

[0021] The method of extracting miRNA-196a, miRNA-155, and miRNA-490 from urine is as follows: take an appropriate amount of urine supernatant and add twice the volume of Trizol reagent, and then perform 2 times of phenol / chloroform extraction, and the obtained RNA is dissolved in 20 μl DEPC water Using microRNA low-density chip detection, take 300 μl sample and add 100 μl DEPC water, mix well, add an equal volume of acidic phenol and chloroform for extraction, and obtain miRNA-196a, miRNA-155, miRNA-490, and then RNA is dissolved in 10 μl DEPC water, Store at -80°C, the pH of acidic phenol is 5.0-5.5, and the volume ratio of acidic phenol to chloroform is 1:1.

[0022] The method of extracting interleukin from urine is as follows: take an appropriate amount of urine and dilute it, add 1 / 4 of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides glomerulosclerosis markers for chronic glomerular diseases and detection kits of the glomerulosclerosis markers, and relates to the technical field of medicine. The glomerulosclerosis markers for the chronic glomerular diseases are miRNA-196a, miRNA-155, miRNA-490 and interleukin in urine, and the detection kits comprise a kit A and a kit B. An analysis proves that focal segmental glomerulosclerosis (FSGS) is more obvious in renal tubular interstitial injury than other glomerular diseases such as MN and MCD, compared with patients with chronic renal tubular interstitial lesions, interleukin in urine of patients with acute or chronic combined acute renal tubular interstitial lesions is obviously increased. Currently, in many renal tubular functional indexes widely adopted clinically at present, the interleukin in the urine can better reflect the acute renal tubular interstitial lesions, a basis is provided for early and specific clinical discovery of the renal tubular interstitial lesions, and early intervention and treatment of the tubular interstitial injury are one of important links for delaying progression of kidney diseases.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a chronic glomerular disease glomerulosclerosis marker and a detection kit thereof. Background technique [0002] Glomerular diseases are a group of diseases mainly involving bilateral glomeruli with different etiology, pathogenesis, clinical manifestations, pathological changes, disease course and prognosis. It can be divided into primary, secondary and hereditary; primary glomerular disease often has unknown etiology, secondary glomerular disease refers to glomerular damage caused by systemic diseases, hereditary glomerular disease is Genetic. Primary glomerular diseases account for the majority of glomerular diseases and are still the main cause of chronic renal failure in my country. [0003] Focal segmental glomerulosclerosis (FSGS) is one of the common causes of nephrotic syndrome (NS) in adults. Due to its complex etiology and unclear pathogenesis, clinical diagnosis an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6883C12N15/113G01N33/68G01N33/577G01N33/535G01N33/532
CPCC12Q1/6883G01N33/6893G01N33/6869G01N33/577G01N33/535G01N33/532C12Q2600/158C12Q2600/178G01N2333/54G01N2800/347
Inventor 朱永俊崔红旺吕婕李晓燕林子艳陈爱高可利杨静
Owner 朱永俊
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap