Method and kit for detecting antibiotic residues in food and production and circulation processes of food

Abstract

The invention discloses a method for detecting antibiotic residues in food and production and circulation processes of the food. The method comprises the following steps: step a, pretreating a sample;step b, preparing a target bacterial liquid; step c, culturing with the kit; step d, determining judgment standard; step e, determining for the first time; and step f, determining for the second time. The method has no restriction on selection of target bacteria, has a screening range covering all antibiotics, simple operation, rapid operation, wide detection range of antibiotics, high sensitivity, high accuracy, less reagent demand, and no need for large-scale analytical instruments, is convenient for inexperienced personnel to operate and use, and has high social significance. Meanwhile, the method is less time-consuming, has low analysis cost, low requirements on experimental environment, strong pertinence and strong anti-interference ability, greatly reduces occurrence of false positives or false negatives, and is suitable for large-scale rapid detection of antibiotic residues in food and production and circulation processes of the food.

Description

technical field [0001] The invention relates to the field of food, in particular to a method and kit for detecting antibiotic residues in food and its production and circulation. Background technique [0002] Human beings use food with a large amount of antibiotic residues, which will cause the human body to develop resistance to antibiotics, cause various tissue and organ lesions, even canceration, and cause great harm to human health. Although the relevant departments of the country have promulgated some standards on the antibiotic content in raw milk, there are still some foods with excessive antibiotic content entering the market, endangering the life and health of consumers. Therefore, the detection of antibiotic residues in food is a very important issue. [0003] Generally speaking, meat (livestock and poultry), fish and shrimp (aquatic), eggs, milk, feed and honey need to be tested for antibiotics. Common methods include chromatographic analysis, microbial detection...

AI Technical Summary

Problems solved by technology

Chromatographic analysis samples require complex pretreatment, slow analysis speed, high requirements for instruments, and high requirements for testing personnel

Microbial detection method is to qualitatively or quantitatively detect the residual antibiotics in the sample according to the inhibitory effect of the sample on microorganisms, but the existing microbial detection method takes a long time to detect, and the detection results need to be judged by the naked eye, which is prone to errors; the immunological detection method mainly relies on The quality of antibody antigen, and the preparation of antigen and antibody is time-consuming and time-consuming, which limits the wide application of immunization method

Moreover, the above three types of existing methods can only detect certain specific types of antibiotics in one test, and cannot cover all antibiotics. The rate of missed detection is high, which is easy to cause false negatives, and the significance of detection is greatly reduced.

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