Unlock instant, AI-driven research and patent intelligence for your innovation.

Batch western blot membrane strip inclination correction and segmentation method and system

A technology for western blotting and tilt correction, which is applied in image analysis, image data processing, instruments, etc., can solve the problems of difficult manual and accurate processing of western blot membrane strips, difficulty in ensuring repeatability and accuracy, and errors, so as to avoid tilting Effects of inaccurate correction, increased correction efficiency, and improved accuracy

Active Publication Date: 2020-12-11
EAST CHINA JIAOTONG UNIVERSITY
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Western blotting is an experience-dependent multi-step method. The quality of the results is affected by many factors. The traditional manual operation is easy to introduce errors, making it difficult to ensure repeatability and accuracy. It is difficult to do it manually accurately, therefore, the combination of western blot strip recognition processing and image processing technology will be an inevitable trend in the future

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Batch western blot membrane strip inclination correction and segmentation method and system
  • Batch western blot membrane strip inclination correction and segmentation method and system
  • Batch western blot membrane strip inclination correction and segmentation method and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Western blot membrane strip in batch and slant correction and segmentation method, comprising the steps of:

[0074] S1: acquiring an image containing a plurality of protein blotting membrane strip, and the image is divided, is eligible

[0075] Have a segmented image;

[0076] S2: obtaining the inclination angle of each of the protein blotting membrane strip after the image segmentation, and protein blotting membrane is corrected corresponding to each bar according to the inclination angle, the inclination obtained preliminary corrected image;

[0077] S3: the initial tilt correction image is preprocessed to obtain a pre-processed image;

[0078] S4: extracting the background region between the plurality of pre-imprinted regions of each protein blotting membrane strip and said plurality of image regions blots, and positioned;

[0079] S5: a plurality of imprinted regions respectively corresponding to each of the protein blotting membrane strip and a plurality of background...

Embodiment 2

[0123] On the basis of Example 1 on a bulk protein blotting membrane strip slant correction and segmentation system comprising: image acquisition module, an image segmentation module, a preliminary correction module, a preprocessing module, a footprint area positioning module, the restructure module;

[0124] The image acquisition module for acquiring an image containing a plurality of film strips Western blot and sent to the image segmentation module;

[0125] The image segmentation module for segmenting said image to obtain a segmented image and sends it to the initial correction module;

[0126] Means for correcting the initial inclination angle of each of the protein blotting membrane strip acquires the image segmentation, and protein blotting membrane is corrected corresponding to each bar according to the inclination angle, the inclination correcting the initial image is obtained and sent to the preprocessing module;

[0127] The pretreatment means for the preliminary pre-ti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a batch western blot membrane strip inclination correction and segmentation method and system, and the method comprises the following steps: S1, obtaining an image containing aplurality of western blot membrane strips, and segmenting the image; S2, obtaining an inclination angle of each western blot membrane strip in the segmented image, and correcting the corresponding western blot membrane strip according to the inclination angle to obtain a preliminary inclination correction image; S3, preprocessing the preliminary inclination correction image; S4, extracting a plurality of imprinting areas of each western blot membrane strip in the preprocessed image, and positioning the imprinting areas; and S5, respectively recombining the plurality of imprinting areas corresponding to each western blot membrane strip to obtain a plurality of recombined western blot membrane strips, and outputting a correction result and position coordinates of the imprinting area corresponding to each western blot membrane strip in the correction result. The defect that inclination correction is inaccurate due to the fact that the space of the film strip is bent can be overcome, andthe correction accuracy is improved.

Description

Technical field [0001] The present invention relates to a protein blotting membrane strip correction technologies, and particularly to a bulk protein blotting membrane strip slant correction and segmentation method and system. Background technique [0002] Western blot membrane strip, i.e. a strip western blotting FIG print on the film strip. "Footprint" or "electro-blotting" refers to the electric field under the influence of a biological sample transferred from the gel to a membrane method. This method requires biomolecular sample may be fixed film for subsequent detection. This surface area, porosity, and protein binding capacity of the membrane are special demands. Western blotting is one modification of the technology, which includes prior to use monoclonal or polyclonal antibody, a protein immobilized on the membrane. Prior to protein immobilization on the membrane, using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to separate native or denatured proteins sample prote...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G06T3/60G06T7/12G06T7/13G06T7/155G06T7/168G06T7/194
CPCG06T3/608G06T7/12G06T7/13G06T7/155G06T7/168G06T7/194G06T2207/20061
Inventor 朱志亮李泽寰罗国亮肖美华涂文
Owner EAST CHINA JIAOTONG UNIVERSITY