Synergistic herbal compositions for the treatment of obesity and overweight
A synergistic composition, the technology of the composition, applied in the direction of drug combination, drug delivery, pharmaceutical formulation, etc., can solve problems such as unacceptable side effects
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example 1
[0083] Example 1: Preparation of an aqueous extract of cocoa seeds
[0084] Cocoa dried seeds (100 g) were crushed and the powdered material was extracted with water (700 mL) in a laboratory extractor for 1 h at room temperature (rt). The extract was filtered and the spent material was re-extracted twice with water (2 x 500 mL) under similar conditions. The combined extracts were filtered and concentrated under vacuum to give the residue of the aqueous extract as a powder (T.C-1; 14 g).
example 2
[0085] Example 2: Preparation of Ethanol and 50% Ethanol-Aqueous Extracts of Cocoa Seeds
[0086] Cocoa dried seeds (100 g) were crushed and the powdered material was extracted with 50% aqueous ethanol (700 mL) in a laboratory extractor for 1 hour at room temperature. The extract was filtered and the spent material was re-extracted twice with 50% aqueous ethanol (2 x 500 mL) under similar conditions. The combined extracts were filtered and concentrated in vacuo to give a residue of the ethanol extract (T.C-2; 11 g).
[0087] An ethanol extract (T.C-3; 4.4 g) of cacao seeds (100 g) was obtained by the same method as above using ethanol as an extraction solvent.
example 3
[0088] Example 3: Preparation of 50% aqueous methanol extract of cocoa seeds
[0089]Cocoa dried seeds (100 g) were crushed and the powdered material was extracted with 50% aqueous methanol (700 mL) in a laboratory extractor for 1 h at room temperature. The extract was filtered and the spent material was re-extracted twice with 50% methanol (2 x 500 mL) under similar conditions. The combined extracts were filtered and concentrated under vacuum to obtain a residue of 50% aqueous methanol extract (T.C-4; 10 g).
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