Saccharopolyspora spinosa capable of highly yielding spinosad and method for increasing spinosad yield of strain

Abstract

The invention provides saccharopolyspora spinosa capable of highly yielding spinosad and a method for increasing the spinosad yield of the strain. The saccharopolyspora spinosa capable of highly yielding the spinosad includes a J1-019-A strain and a spinosad producing strain saccharopolyspora spinosa capable of overexpressing spnJ or spnP; according to the J1-019-A strain, a polyketide synthase gene cluster which is not related to spinosad synthesis is knocked out from a genome of an original spinosad producing strain saccharopolyspora spinosa, and an attB site which can be conveniently transformed subsequently is introduced; and the spinosad producing strain saccharopolyspora spinosa capable of overexpressing spnJ or spnP performs overexpression on the spnJ or spnP in the original spinosad producing strain saccharopolyspora spinosa. The spinosad producing strain provided by the invention can realize fermentation production of spinosad through amplified fermentation of a fermentation tank, and can achieve the potential of industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to Saccharopolyspora spinosa with high spinosad production and a method for increasing the spinosad yield of the strain. Background technique [0002] Spinosad is a macrolide biopesticide produced by the actinomycete Saccharopolyspora spinosa through aerobic fermentation. Because spinosad is fast, efficient, low residue, Non-toxic to mammals and birds, the insecticide has been approved for registration and use in many countries, and has won the "Presidential Green Chemicals Challenge Award" in the United States. [0003] However, at present, many countries do not have the ability to produce spinosad by fermentation. The main reason is that the fermentation period of this strain is long and the fermentation yield is low, so it is difficult to implement industrialization. At present, it is a common solution to use biotechnology to transform fermentation strains to increase production and...

AI Technical Summary

Problems solved by technology

However, although the wild-type transformation of this strain is relatively easy, there are also reports on the transformation of the wild-type strain of this strain, but because the fermentation yield of the wild-type strain is very low, it is difficult to apply it to industrial production even if the yield is improved after transformation.

For the production strain of S. spinosyn that has undergone multiple rounds of mutagenesis, its genetic manipulation is almost infeasible. Therefore, the industrialization of the spinosad strain is seriously hindered. It can accelerate the industrialization process of spinosad fermentation

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