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Cherry gummosis bacteria inhibitor and preparation method thereof

The technology of a bacteriostatic agent and a preparation method is applied in the field of a bacteriostatic agent for cherry gum flow bacteria and its preparation, and achieves the effects of non-toxicity in prevention and control costs and reduction in prevention and control costs.

Inactive Publication Date: 2021-01-08
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no good control measures in the world. It is necessary to combine chemical methods, physical methods and biological methods for comprehensive prevention and control, which is an effective measure to inhibit the occurrence of fruit tree gummosis, promote the growth of fruit trees, and improve fruit yield and quality.

Method used

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  • Cherry gummosis bacteria inhibitor and preparation method thereof
  • Cherry gummosis bacteria inhibitor and preparation method thereof
  • Cherry gummosis bacteria inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Pour the slightly cooled PDA medium after sterilization into a petri dish (diameter 9cm) to make a plate, use a puncher (diameter 6mm) to punch a hole in the center of the plate, and the inhibitor (proportioning: fresh garlic 10g, fresh Onion 10g, cinnamon 10g, fennel 10g, crushed and distilled to 2000ml with distilled water, let stand for leaching for 12 hours, and the filtrate is used for later use) Add to the hole, inoculate the bacteria plate of cherry tree gummosis bacteria around the hole, put it in 28℃ for constant temperature cultivation Box culture, with the treatment of adding sterilized water as the control, each treatment was repeated 5 times. When the control plate was full, the size of the inhibition zone of each treatment was measured and statistically analyzed.

Embodiment 2

[0015] Pour the slightly cooled PDA medium after sterilization into a petri dish (diameter 9cm) to make a plate, punch a hole in the center of the plate with a puncher (diameter 6mm), and mix the inhibitor (proportioning: fresh garlic 15g, fresh Onion 15g, cinnamon 15g, fennel 15g, crushed and distilled to 1500ml with distilled water, let stand for leaching for 48 hours, and the filtrate is used for later use) into the hole, inoculate cherry tree gelatinous bacteria plate around the hole, and put it into 28 ℃ constant temperature culture Box culture, with the treatment of adding sterilized water as the control, each treatment was repeated 5 times. When the control plate was full, the size of the inhibition zone of each treatment was measured and statistically analyzed.

Embodiment 3

[0017] Pour the slightly cooled PDA medium after sterilization into a petri dish (diameter 9cm) to make a plate, punch a hole in the center of the plate with a puncher (diameter 6mm), and mix the inhibitor (proportioning: fresh garlic 30g, fresh 30g of onion, 30g of cinnamon, 30g of fennel, crushed and distilled to 1000ml with distilled water, let stand for leaching for 36 hours, and the filtrate will be used for later use) into the hole, inoculate the bacteria tray of cherry tree gummosis bacteria around the hole, and put it in a constant temperature culture at 28°C Box culture, with the treatment of adding sterilized water as the control, each treatment was repeated 5 times. When the control plate was full, the size of the inhibition zone of each treatment was measured and statistically analyzed.

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Abstract

The invention discloses a cherry gummosis bacteria inhibitor and a preparation method thereof, and belongs to the technical field of biology. The preparation method for solving the technical problem comprises the following steps: crushing 10-30 parts of garlic, 10-30 parts of onion, 10-30 parts of cinnamon and 10-30 parts of fennel for later use, then adding distilled water into the crushed raw materials, standing and leaching for 12-48 hours at a temperature of 20-35 DEG C, and filtering to obtain the bacteriostatic agent. According to the invention, reagents used in the method are free of toxic and side effects and belong to environment-friendly inhibitors, the effect of inhibiting gummosis bacteria of cherry trees is equivalent to the antibacterial effect of lime sulfur, the preventionand treatment cost can be reduced, and the cherry gummosis bacteria inhibitor is non-toxic, harmless and free of side effects and is expected to become an optional medicament for preventing and treating gummosis of cherry trees.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a bacteriostatic agent for cherry gum flow bacteria and a preparation method thereof. Background technique [0002] Gum flow is a common disease of certain fruit trees, especially drupe fruit trees such as cherries, peaches, apricots, and plums. It also occurs in apple orchards in recent years. Once the fruit tree suffers from the disease, it will begin to flow gum, and the tree vigor will gradually decline. When the disease is serious, it can cause the destruction of the entire orchard, and the yield and quality of the fruit trees will also be seriously affected. In the past, people thought that fruit tree gum disease was a physiological disease, but later people realized that the cause of fruit tree gum disease has both biological and abiotic factors. At present, there are no good control measures in the world. It is necessary to combine chemical methods, physical methods,...

Claims

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Application Information

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IPC IPC(8): A01N65/42A01P3/00
CPCA01N65/00A01N65/10A01N65/24A01N65/42
Inventor 侯义龙庄杰
Owner DALIAN UNIV