Multi-protein combined biomarker, application and heart valvular disease complicated with atrial fibrillation diagnostic kit

A biomarker and atrial fibrillation technology, applied in the field of molecular biology, can solve time-consuming and labor-intensive problems

Pending Publication Date: 2021-01-12
TEDA INT CARDIOVASCULAR HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Protein is the main bearer of life activities. It not only participates in the construction of cell structural components, but also participates in almost all cell...

Method used

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  • Multi-protein combined biomarker, application and heart valvular disease complicated with atrial fibrillation diagnostic kit
  • Multi-protein combined biomarker, application and heart valvular disease complicated with atrial fibrillation diagnostic kit
  • Multi-protein combined biomarker, application and heart valvular disease complicated with atrial fibrillation diagnostic kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0173] 1. Collection of Human Plasma Samples

[0174] Patients with heart disease and atrial fibrillation were the experimental group, and patients who maintained sinus rhythm were the control group. Blood samples were collected from all patients (experimental group and control group) in the morning before the operation, under the condition of fasting>10h. 2ml of whole blood was collected from each patient's peripheral vein, and immediately centrifuged at 2500rpm for 10 minutes to separate the upper layer of plasma. The separated plasma was then divided into several equal parts, placed in plasma collection tubes, and stored in a -80°C refrigerator for testing.

[0175] 2. Proteomic detection

[0176] 1) Plasma proteome detection

[0177] Proteins in each mixed sample of the two groups were detected by iTRAQ combined with multidimensional liquid chromatography-mass spectrometry (LC-MS / MS) (Applied Biosystems, Foster City, CA).

[0178] 2) Sample preparation

[0179] a) Pur...

Embodiment 2

[0235] Enzyme-linked immunosorbent assay (ELISA)

[0236] Apolipoprotein A1, angiotensinogen, LIM domain protein 7, macrophage colony-stimulating factor receptor 1, vitronectin, and prothrombin fragment F1+2 were detected by ELISA in freshly collected plasma samples. verified.

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Abstract

The invention provides a multi-protein combined biomarker, application and a heart valvular disease complicated with atrial fibrillation diagnostic kit. The multi-protein combined biomarker is composed of an apolipoprotein A1 protein antibody, an angiotensin antigen antibody, an LIM structural domain protein 7, a macrophage colony stimulating factor receptor 1, a vitronectin antibody and a prothrombin fragment F1 + 2 antibody. Modern biological technology and bioinformatics analysis find that combined application of the six proteins has the application of predicting valvular heart disease complicated with atrial fibrillation, can be applied to preparation of targeted drugs for early intervention of related diseases, and can also be applied to preparation of a heart valvular disease complicated with atrial fibrillation screening kit. According to the kit, modeling is carried out after the concentrations of the six proteins are measured, and good evaluation efficiency and potential hugeapplication value are achieved on whether atrial fibrillation happens to a patient suffering from the heart valvular disease or not.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a polyprotein combination biomarker and its application and a diagnostic kit for heart valve disease complicated with atrial fibrillation. Background technique [0002] Atrial fibrillation (AF, AF) is one of the most common cardiac arrhythmias. After the occurrence of atrial fibrillation, the effective contraction of the atrium is lost, the hemodynamics changes, atrioventricular dyssynchrony, progressive atrioventricular dysfunction and thromboembolic complications occur, and the mortality rate of patients increases. At the same time, atrial fibrillation is related to the occurrence of stroke, myocardial infarction, heart failure, dementia, chronic kidney disease and other diseases, and the combination of atrial fibrillation can increase the mortality of the disease. With the acceleration of population aging, the number of patients with atrial fibrillation will increase signific...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/326
Inventor 何国伟李明阳刘晓程陈焕新侯海涛王君杨沁
Owner TEDA INT CARDIOVASCULAR HOSPITAL
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