Supercharge Your Innovation With Domain-Expert AI Agents!

Application of ethyl hydroxybenzoate compounds as nematicide preparations

A technology of ethyl hydroxybenzoate and nematicide, which is applied in the application field of ethyl hydroxybenzoate compound as a nematode preparation, can solve the problem of high toxicity, less than 30% lethality, and failure to effectively prove the killing of pine wood nematodes. Insect activity and other issues, to achieve the effect of improving activity

Active Publication Date: 2021-01-19
沈阳恩柽研究院有限公司
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of December 2019, there were 312 nematicide products registered in my country, and the active ingredients were mainly concentrated in abamectin, thiazophos, isofenphos-methyl, methimenphos, methyl bromide, aldicarb, amino-oligosaccharins, Metron, cartap, carbamur, carbofuran, carbosulfan, etc., most of the products are organophosphorus products, which are highly toxic, and the target organisms from research and development to promotion of this type of pesticide mainly target the harmful Nematodes of herbaceous plants (such as root-knot nematode), most of the pesticides have a general inhibitory effect on pine xylophilus parasitic in woody plants such as pinaceae. For example, the lethality rate of abamectin to pine xylophilus at the recommended concentration is less than 30%. %
Therefore, because pine xylophilus and Caenorhabditis elegans are far apart (belonging to different subclasses), they have extremely significant differences in biological characteristics, pathogenicity, degree of damage, tolerance to pesticides, etc. , so that the pesticides and compounds that have been reported to have insecticidal activity against Caenorhabditis elegans cannot convincingly prove their insecticidal activity against pine xylophilus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ethyl hydroxybenzoate compounds as nematicide preparations
  • Application of ethyl hydroxybenzoate compounds as nematicide preparations
  • Application of ethyl hydroxybenzoate compounds as nematicide preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, the safety detection of the bioassay system for pine xylophilus

[0035] Drug preparation: use sterilized water to prepare an aqueous solution of the tested drug, in which the content of DMSO is 4%, and the content of Tween 80 is 15%; the sterilized water without adding the drug is used as a control.

[0036] Preparation of the nematode suspension: Suspend the pine wood nematode on the surface of a 9cm PDA culture dish with an appropriate amount of sterilized water, and then separate the live pine wood nematode suspension through the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in the J2 and J4 ...

Embodiment 2

[0040] Conclusion: The corrected mortality rate of 4% DMSO+15% Tween 80 aqueous solution to pine xylophilus 24h is 0.01%, far lower than 5%, which proves that 4% DMSO+15% Tween 80 aqueous solution is safe to pine xylophilus and can be used as Bioassay system application of pine xylophilus test object. Example 2, Toxic activity and LC50 determination of ethyl 2-hydroxybenzoate to pine xylophilus

[0041] Medicament preparation: Ethyl 2-hydroxybenzoate was formulated into 5 concentrations of 0, 0.1, 1, 5, and 10 g / L with a bioassay system (4% DMSO+15% Tween aqueous solution).

[0042] Preparation of the nematode suspension: Suspend the pine wood nematode on the surface of a 9cm PDA culture dish with an appropriate amount of sterilized water, and then separate the live pine wood nematode suspension through the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilize...

Embodiment 3

[0047] Example 3, Toxic activity and LC50 determination of ethyl 4-hydroxybenzoate to pine xylophilus

[0048] Medicament preparation: Ethyl 4-hydroxybenzoate was prepared into 5 concentrations of 0, 0.1, 1, 5, and 10 g / L with a bioassay system (4% DMSO+15% Tween aqueous solution).

[0049] Preparation of the nematode suspension: Suspend the pine wood nematode on the surface of a 9cm PDA culture dish with an appropriate amount of sterilized water, and then separate the live pine wood nematode suspension through the Bellman funnel method, and dilute the nematode suspension at 2000r / Centrifuge for 2 min, remove the supernatant and keep the sediment, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes under the microscope, and finally quantify the nematode suspension to 1000 nematodes / mL with sterilized water. The nematodes are in the J3 stage, and a few are in the J2 and J4 stages, and they are ready for use at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of pesticides, and particularly relates to an application of ethyl hydroxybenzoate compounds as nematicides. The invention discloses application of one or more of ethyl hydroxybenzoate compounds represented by a general formula I in serving as the nematicides. The general formula I is shown in the specification. The compounds are suitable for being used as the nematicides, especially ethyl 3-hydroxybenzoate and ethyl 4-hydroxybenzoate which are good in activity, are antioxidants widely applied at present, and the compounds are low in toxicity, low in price and easy to obtain.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to the application of ethyl hydroxybenzoate compound as a nematode (especially Bursaphelenchus xylophilus) preparation. Background technique [0002] Nematodes, belonging to the phylum Nematodes, are a class of bilaterally symmetrical protocoelomates; commonly live in land, oceans, and lakes; the species is the second largest group of invertebrates after insects, accounting for the largest number of multicellular organisms on Earth 4 / 5 of nematodes have been observed and described. There are about 23,000 species of nematodes, 256 families and 2271 genera have been identified, and there are still 1 million species to be discovered. In 1743, British scientist Needham found wheat grains in wheat diseased grains After nematodes, various soil nematodes have been reported in various parts of the world. In 1993, Yeates and other scholars divided the nutritional groups of soil nematod...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/40A01N43/90A01P5/00
CPCA01N37/40A01N43/90
Inventor 李世友赵福强李帅强王萍韩璐
Owner 沈阳恩柽研究院有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com