Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke

An ischemic stroke and ectoine technology, which is applied to the application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke, and the new application field of ectoine substances, which can solve the problem of The application of ketone is not found and other problems

Active Publication Date: 2021-08-10
BLOOMAGE BIOTECHNOLOGY CORP LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no discovery about the application of ectoine in the prevention and treatment of ischemic stroke.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke
  • Application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke
  • Application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Effects of ectoine-like substances on the inflammatory response of microglial cells induced by LPS

[0022] The effect of ectoine-like substances on LPS was investigated by referring to the method of "the effect of ethanol extract of striated hippocampus on lipopolysaccharide-induced BV2 microglial inflammatory response" (Journal of Guangdong Ocean University, 2019, 39(1):90-96). Effects on Induction of Microglial Inflammatory Responses. details as follows:

[0023] 1. Test method:

[0024] 1.1 Cell culture:

[0025] Microglial BV2 cells were inoculated in DMEM high-glucose medium containing 10% fetal bovine serum (containing 1% double antibody) at 37°C, 5% CO 2 Cultured in an incubator with saturated humidity, passaged every 2-3 days, when BV2 cells adhered to the wall and grew well, they were seeded in 24-well plates for subsequent experiments.

[0026] 1.2 Griess method was used to detect the NO content released by cells in each group:

[0027] Take t...

Embodiment 2

[0039] Embodiment 2: the protective effect of ectoine on neurons:

[0040] Refer to the method of patent CN109908154A to investigate the protective effect of ectoine on neurons, as follows:

[0041] 1. Test method:

[0042] 1.1 Establishment of the oxygen-glucose deprivation / reoxygenation (OGD / R) model of rat primary cortical neurons:

[0043] Sodium dithionite (Na 2 S 2 o 4 ) dry powder to make it fully dissolved, so that the final concentration is 20mM, with NaHCO 3 Adjust the pH to 7.2, which is the anoxic solution. The primary cortical neuron cells were cultured in a 24-well cell culture plate for 7-10 days, and the cells in good growth state were selected for experiments. The experiment included blank control group, model control group and ectoine administration group.

[0044]The blank control group continued to culture with the medium, removed the medium after 24 hours, and added an equal volume of maintenance medium (Neurobasal Media 49ml+B27-Supplement 1ml); th...

Embodiment 3

[0051] Example 3: The protective effect of ectoine on cerebral infarction induced by ischemia-reperfusion in rats

[0052] With reference to the method of patent CN109908154A, the protective effect of ectoine on cerebral infarction induced by ischemia-reperfusion in rats was investigated, as follows:

[0053] 1. Test method:

[0054] 1.1 Construction of cerebral infarction model:

[0055] The cerebral infarction model was established by blocking the middle cerebral artery of SD rats with suture and performing ischemia-reperfusion.

[0056] 1.2 Test grouping and treatment:

[0057] The successfully modeled SD rats were randomly divided into 3 groups: the model group, the positive drug group and the ectoine group; among them, the positive drug group was injected with Ginadol through the tail vein at a dose of 15 mg / kg, and the ectoine group was Ecdoine was injected into the tail vein at a dose of 15 mg / kg, and the model group was given the same amount of normal saline for 3 c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of ectoine substances in the preparation of drugs for preventing and treating ischemic stroke, and belongs to the technical field of medical applications. The present invention finds for the first time that ectoine substances can reduce the inflammatory response of microglia induced by LPS, and have protective effects on neurons and cerebral infarction induced by ischemia-reperfusion in rats, and can be used to develop new drugs for preventing or treating atherosclerosis. Drugs for hemorrhagic stroke.

Description

technical field [0001] The invention relates to a new application of ectoine-like substances, in particular to the application of ectoine-like substances in the preparation of medicines for preventing and treating ischemic stroke, and belongs to the technical field of medical applications. Background technique [0002] Stroke is a sudden cerebral blood circulation disorder disease. Due to the reduction of cerebral blood flow supply, it causes the lack of oxygen and nutrients in brain tissue, which eventually leads to neuronal cell death and cerebral infarction. Ischemic stroke accounts for more than 85% of all strokes, and its proportion in China is increasing year by year, and it is becoming younger. It has become the number one cause of disability and death in my country, causing serious economic and social burdens. In addition to high disability rate and high mortality rate, stroke also has the characteristics of high incidence, high recurrence rate and many complications...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/505A61P9/10
CPCA61K31/505A61P9/10
Inventor 钱林艺田昕董杰杰郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD