Low-damage plant xylem juice sampling method
A xylem, low-damage technology, applied in sampling devices, instruments, measuring devices, etc., can solve the problems of plants not surviving, plant damage, etc., and achieve the effect of small damage to plants, less damage, and faster recovery
Active Publication Date: 2021-01-26
JIANGSU UNIV
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Problems solved by technology
[0010] Aiming at the problem that the above-mentioned sampling technology is extremely harmful to the plants and the plants cannot survive after sampling, the present invention proposes a method according to the structural characteristics of the plant stem section A low-damage plant xylem juice sampling method, the sampling method has the characteristics of easy operation, less pollution, less damage to plants, and plants can survive after sampling
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[0028] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.
[0029] In this embodiment, preferably, a quanta200 scanning electron microscope system produced by Fei Company of the United States is used, and the microscopic image acquisition system in the scanning electron microscope system is used to collect the cross-sectional microstructure of cucumber stalks in the greenhouse. The experimental site of the present invention is the glass greenhouse of the Key Laboratory of Modern Agricultural Equipment and Technology of the Ministry of Education of Jiangsu University. The experimental objec...
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Abstract
The invention provides a low-damage plant xylem juice sampling method which comprises the following steps: observing the microstructure of the cross section of a plant stem through a scanning electronmicroscope, and determining the distribution characteristic, structural characteristic and geometric dimension of xylem on the cross section of the stem; determining the position and the depth of a plant stalk incision by combining the sampling requirement with the distribution characteristics, the structural characteristics and the geometric dimension of the xylem; according to the incision position and depth determined in the second step, rapidly cutting a groove in the plant stem through the disinfected blade; sucking xylem juice overflowing from the notch below the groove by using a pipettor, transferring the juice into a sample tube, collecting sufficient xylem juice samples, and putting the samples into liquid nitrogen for subsequent experiments; treating plant stalk wounds, and increasing the recovery speed. The method is easy to operate, little in pollution and small in damage to plants, and the sampled plants can survive.
Description
technical field [0001] The invention belongs to the technical field of plant juice sampling, in particular to a low-damage plant xylem juice sampling method. Background technique [0002] Xylem sap is mainly composed of water, mineral nutrients and various organic substances synthesized by plant roots, and its composition and content vary according to plant species. Water in xylem sap is an essential medium for plant growth and development. Plants can carry out various physiological activities when the water content is high enough. In various biochemical reactions, water plays the role of medium or solvent. At the same time, the vaporization of water will also take away a lot of heat to stabilize body temperature. There are generally 17 kinds of mineral nutrients in xylem sap, including N, P, K, Zn, Fe, Cu, etc., most of which are absorbed by roots from soil or culture medium. There are two main aspects of the role of mineral elements, one is used to synthesize its own str...
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IPC IPC(8): G01N1/04G01N1/10G01N23/2251
CPCG01N1/04G01N1/10G01N23/2251
Inventor 张文涛李青林徐慧孙京京
Owner JIANGSU UNIV