Early warning method for liver cancer, detection kit for early warning and detection method

A detection kit and technology for liver cancer, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of high cost, poor sensitivity and specificity of early screening and early warning of liver cancer, and achieve low cost, The effect of high coincidence degree and increased survival rate

Pending Publication Date: 2021-01-29
苏州天隆生物科技有限公司
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Problems solved by technology

[0005] One of the purposes of the present invention is to provide an early warning method for liver cancer, which solves th...

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  • Early warning method for liver cancer, detection kit for early warning and detection method
  • Early warning method for liver cancer, detection kit for early warning and detection method
  • Early warning method for liver cancer, detection kit for early warning and detection method

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Embodiment 1

[0053] This embodiment discloses a method for detecting DNA methylation of RASSF1A, ELF, p16 and GSTP1 genes in peripheral blood samples using the detection kit of the present invention, specifically:

[0054] 1. Separation and storage of plasma samples: Take 10mL of peripheral blood from the subject into anticoagulant blood collection tubes, centrifuge at 3000rpm for 10min to separate the samples into three layers: plasma, white blood cells and red blood cells, and take out the most blood in the anticoagulant tube with a pipette. Put the upper plasma into a new centrifuge tube, mark it and store it at 80°C.

[0055] 2. Plasma free DNA (cfDNA) extraction: Plasma free DNA extraction and transformation kits were used for extraction, and all operations were strictly performed in accordance with the kit instructions.

[0056] 3. cfDNA conversion: Use the plasma free DNA extraction and conversion kit to modify and purify the extracted cfDNA with bisulfite, and operate strictly acco...

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Abstract

The invention discloses an early warning method for liver cancer, a detection kit for early warning and a detection method, and belongs to the technical field of biology. The early warning method comprises the following steps: detecting DNA methylation levels of RASSF1A, ELF, p16 and GSTP1 genes in a peripheral blood sample through a PCR method, calculating a risk value of suffering from liver cancer, and carrying out early warning according to a calculation result. The calculation formula is as follows: Y is equal to 1.208163-0.00653*Ct(RASSF1A)-0.00751*Ct(ELF)-0.00555tCT(p16)-0.00457*Ct(GSTP1), wherein when Y is larger than or equal to 0.3177, the risk is judged to be high, and when Y is smaller than 0.3177, and the risk is judged to be low. The multi-gene methylation joint detection kitfor early warning of liver cancer comprises a DNA methylation detection reagent for specifically detecting the RASSF1A, ELF, p16 and GSTP1 genes in the peripheral blood sample, an endogenous gene andan endogenous gene detection reagent. The high-risk early warning result and the clinical diagnosis result are high in conformity, good application prospects are achieved for diagnosis of early livercancer, the survival rate of liver cancer patients is increased, and the cure rate of liver cancer patients is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an early warning method for liver cancer, a detection kit for early warning, and a detection method. Background technique [0002] Hepatocellular Carcinoma (HCC) is a primary liver cancer with a high mortality rate, and it is one of the most common malignant tumors worldwide, especially in Asia, Africa and southern Europe. Worldwide, hundreds of thousands of people die from liver cancer every year. The World Health Organization (WHO) clearly points out that if cancer patients can be detected early, the cure rate can reach 80%. Therefore, the early diagnosis of liver cancer is of great significance to the treatment effect and quality of life of liver cancer patients. [0003] In the prior art, methods for early screening and diagnosis of liver cancer are mainly divided into imaging examination and biochemical detection. Among them, the biochemical detection based on the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6858
CPCC12Q1/6886C12Q1/6858C12Q2600/154C12Q2600/166C12Q2531/113C12Q2523/125C12Q2545/101C12Q2563/107
Inventor 苗保刚韩文孙瑶罗淑芬
Owner 苏州天隆生物科技有限公司
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