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Rescue method for damping-off seedlings in culture process of peach embryogenic seedlings

A rescue method and embryo culture seedling technology are applied in the field of rescue of blight seedlings during the cultivation of peach embryo seedlings, which can solve the problem of high incidence of blight disease, achieve low cost, simple and practical operation, and improve survival rate. Effect

Active Publication Date: 2021-02-05
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for rescuing diseased seedlings with blight during the cultivation process of peach embryo seedlings, to solve the problem of higher incidence of blight after embryo rescued seedlings are transplanted, and to effectively save rare resources descendants of

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  • Rescue method for damping-off seedlings in culture process of peach embryogenic seedlings
  • Rescue method for damping-off seedlings in culture process of peach embryogenic seedlings
  • Rescue method for damping-off seedlings in culture process of peach embryogenic seedlings

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Embodiment 1

[0029] Prevention and control of damping-off disease during the first transplanting of seedlings

[0030] Hybrid combination 1 is Huyou 018*Huyou 002, hybrid combination 2 is Jinxiang*Jinxia Youpantao, and hybrid combination 3 is Chunxiao*Zhongyoutao 9.

[0031] The matrix formula is peat: perlite: vermiculite volume ratio 2:1:1.

[0032] For treatment A, after mixing the substrate with wetness, use an autoclave to sterilize it for 20 minutes, put it into a single nutrient bowl after cooling, transplant the test-tube seedlings, and pour 800 times of carbendazim solution after transplanting the seedlings, then cover with a disposable plastic cup to moisturize, put Grow in a greenhouse. After the seedlings survived, the plastic cups were removed. The soil should not dry out and not watered. Two weeks after planting, the cotyledons began to wilt and become moldy ( figure 1 ), cut off all cotyledons from the base and take them outside the greenhouse for centralized destruction...

Embodiment 2

[0039] Rescue of the stem blighted plants during the second seedling transplantation

[0040] Hybrid combination 1 is Huyou 018*Huyou 002, hybrid combination 2 is Jinxiang*Jinxia Youpantao, and hybrid combination 3 is Chunxiao*Zhongyoutao 9.

[0041] The matrix formula is peat: perlite: vermiculite volume ratio 2:1:1. After the matrix is ​​wetted, use an autoclave to sterilize it for 20 minutes, put it into a single nutrient bowl after cooling, transplant the test-tube seedlings, and water the carbendazim 800 times after transplanting, then cover the disposable plastic cup to moisturize, and put it in the greenhouse nourish. After the seedlings survived, the plastic cups were removed. The soil should not dry out and not watered. Two weeks after planting, the cotyledons began to wilt and become moldy, and all cotyledons were cut off from the base and taken outside the greenhouse for centralized destruction. If the infected seedlings are found, immediately remove them from t...

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Abstract

The invention provides a rescue method for damping-off seedlings in the culture process of peach embryogenic seedlings, and relates to the technical field of plant planting. According to the rescue method, the diseased seedlings are moved to an open air environment from the first seedling transplanting, the selection of independent nutrition pots, the disinfection of matrices, the use of bactericides and the removal of cotyledons of the seedlings to the second seedling transplanting, resources are stored through various methods such as grafting, cutting or cutting back to promote sprouting, and diseased plants are rescued. According to the rescue method, 50-60% of the number of diseased seedlings can be converted into healthy seedlings, and therefore the final survival rate of embryo rescue seedlings is greatly increased. According to the rescue method, the survival rate of the embryo rescue seedlings can be increased from 50-60% to 70-80% by controlling each link; by means of the method, progenies of some rare resources can be effectively saved; and the method is simple, effective, low in cost, simple to operate and practical.

Description

technical field [0001] The invention belongs to the technical field of plant planting, and in particular relates to a method for rescuing seedlings with blight blight during the cultivation process of peach embryo seedlings. Background technique [0002] Damping blight, also known as damping-off, is a worldwide seedling disease, mainly causing damage to the stem base or young roots of seedlings. The disease mainly affects the base of seedling stems or young roots. After the seedlings are unearthed, the base of the stem turns brown and appears water-soaked, and the diseased part constricts, wilts and dies but does not collapse. The young roots are rotten, and the diseased part is light brown, with a white cotton-like or spider-like mycelium layer, which is the mycelium or sclerotia of the pathogen. The pathogen is called Rhizoctonia solani Kühn (Rhizoctonia solani Kühn), which belongs to the half-known fungi. Occurrence law: Mycelium can directly invade the host and spread...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G2/10A01G2/30A01G31/00A01H4/00A01N43/80A01N37/34A01N47/18A01P3/00
CPCA01G17/005A01G2/10A01G2/30A01G31/00A01H4/005A01N43/80A01N37/34A01N47/18
Inventor 杜纪红叶正文苏明申周慧娟李雄伟张夏南
Owner SHANGHAI ACAD OF AGRI SCI