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Puccinia triticinia resistant protein as well as coding gene and application thereof

A leaf rust and wheat technology, applied to wheat leaf rust resistance protein and its coding gene and application fields, can solve the problems of easy loss of resistance, single variety, easy to overcome resistance, etc., and achieves improved resistance and strong specificity. , the effect of high sensitivity

Active Publication Date: 2021-02-05
HEBEI AGRICULTURAL UNIV.
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] In view of the fact that there is a single variety of wheat resistant to leaf rust in wheat, its resistance is easy to be overcome during the long-term mutation process of the pathogen, and most of the current leaf rust resistance genes are race-specific resistance genes, which vary with the toxicity of the race. The problem of easy loss of resistance due to variation, the present invention provides a wheat leaf rust resistance protein and its coding gene and application

Method used

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  • Puccinia triticinia resistant protein as well as coding gene and application thereof
  • Puccinia triticinia resistant protein as well as coding gene and application thereof
  • Puccinia triticinia resistant protein as well as coding gene and application thereof

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Embodiment 1

[0025] 1. Materials

[0026] Common wheat variety Zhoumai 22 (ZM): a Chinese wheat landrace with high resistance to leaf rust;

[0027] Wheat variety Zhengzhou 5389 (ZZ): a variety highly susceptible to leaf rust;

[0028] Wheat variety Fielder: a material variety susceptible to leaf rust;

[0029] Leaf rust pathogenic fungi: Puccinia triticinia, FHDS physiological race.

[0030] 2. Grading standards for wheat leaf rust resistance

[0031] According to the phenotype, the grading standard of 0-4 was adopted for the infection type of the leaf rust resistance of the plants. "0" means immunity, ";" means near immunity, "1" means high disease resistance, "2" means moderate disease resistance, "3" means moderate disease resistance, "4" means high disease resistance. According to the classification, 0-2 is disease-resistant, and 3-4 is susceptible. The phenotypes corresponding to each grading standard are shown in Table 1.

[0032] Table 1

[0033]

[0034]

[0035] 3. A...

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Abstract

The invention relates to the technical field of biology, and particularly discloses a Puccinia triticinia resistant protein as well as a coding gene and application thereof. The Puccinia triticinia resistant protein has an amino acid sequence as shown in SEQ ID NO: 1, or has an amino acid sequence which is obtained by deleting, replacing or adding one or more amino acids to the amino acid sequenceas shown in SEQ ID NO: 1 and has a function of improving Puccinia triticinia resistance. The Puccinia triticinia resistant protein or the coding gene thereof can be applied to regulation or improvement of Puccinia triticinia resistance. The invention provides the Puccinia triticinia resistant protein, and particularly discloses the amino acid sequence of the Puccinia triticinia resistant protein,resistance of wheat to Puccinia triticinia can be remarkably improved by improving expression quantity of the Puccinia triticinia resistant protein in wheat, and the Puccinia triticinia resistant protein has great application and popularization value for Puccinia triticinia resistance breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a wheat leaf rust resistance protein and its coding gene and application. Background technique [0002] Wheat is one of the most important food crops and a major source of food worldwide. However, wheat rust has been threatening wheat production for thousands of years and has become the main disease of wheat production. Wheat rust is divided into wheat leaf rust (Puccinia triticinia), wheat stem rust (P.graminis f.sp.tritici) and wheat stripe rust (P.striiformis f.sp.tritici). Among them, wheat leaf rust caused by P. tritici is relatively common and occurs most widely in the world. It is one of the important factors that endanger wheat production. In severe epidemic years, wheat production can be reduced by more than 40%, or even crop failure. Wheat leaf rust mainly occurs in the Yangtze River Basin, Huanghuai River Basin, North China wheat region and Northeast wheat region, etc. I...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K19/00C12N15/82A01H5/00A01H6/46C12Q1/6895C12N15/11
CPCC07K14/415C12N15/8282C12Q1/6895C07K2319/20C07K2319/21C07K2319/43C07K2319/22C07K2319/41C07K2319/42C12Q2600/13
Inventor 李在峰闫晓翠李淼淼张培培殷贵鸿刘志勇刘大群
Owner HEBEI AGRICULTURAL UNIV.
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