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A ginsenoside and nanotechnology, applied in the field of preparation of nanometer ginsenoside CK, can solve the problems of limited efficacy, poor oral absorption, low solubility of ginsenoside CK, etc., and achieve the effects of improving water solubility and enhancing drug efficacy.
Inactive Publication Date: 2021-02-09
广东启山生物科技有限公司
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Problems solved by technology
[0005] However, ginsenoside CK has low solubility and poor oral absorption, which limits its efficacy
Method used
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Embodiment 1
[0029] Embodiment 1: the preparation of nanometer ginsenoside CK
[0030] Prepare each polymer micelle by film hydration method, weigh 100mg each of PEG-PLA2k / 2k, PEG-PLA5k / 5k, 1-10mg of ginsenoside CK, add 1-50mL of methanol to dissolve, and rotate at 25-60℃ under reduced pressure Evaporate to form a uniform transparent film, add 1-20mL deionized water to shake, and filter through a 0.22 μm microporous membrane to obtain PEG-PLA nanomicelles with different drug loadings loaded with ginsenoside CK. Freeze-dried for later use. The preparation method of nano ginsenoside containing azone (NT) is as follows: mixing ginsenoside with 0.1% NT, and preparing nano micelles by film-forming method. For the stability of nano-ginsenosides, see figure 1 .
Embodiment 2
[0031] Example 2: Capability of Nano Ginsenosides to Free Radicals
[0032] To culture human melanocytes, add 0, 10, 50, 100, 250, 500, 1000 μg / mL free CK and 4 kinds of nano-CKs to the culture plate, culture at 37°C for 48 hours, and detect the ability of different nano-ginsenosides to capture free radicals, see figure 2 .
Embodiment 3
[0033] Embodiment 3: melanin content
[0034] Human melanocytes were treated with different concentrations of CK and four nano-CKs (0, 10, 100, 1000 μg / mL) for 72 hours. Collect the cells, dissolve them in 200 μL of a solution containing 1NNaOH and 10% DMSO, treat them at 100°C for 30 minutes, centrifuge at 13,000 rpm for 10 minutes, and use a microplate reader to analyze the melanin content at 415nm, see image 3 .
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Abstract
The invention discloses a preparation method of nano ginsenoside CK, and relates to the technical field of cosmetics. Each polymer micelle is prepared through a film hydration method: respectively weighing 100mg of PEG-PLA<2k/2k>, 100mg of PEGPLA<5k/5k> and 0-8mg of ginsenoside CK, adding 1-50mL of methanol to dissolve, carrying out reduced pressure rotary evaporation at 25-60 DEG C to form a uniform transparent film, adding 1-20mL of deionized water for oscillation, performing filtering through a 0.22[mu]m millipore filter membrane to obtain ginsenoside CK-loaded PEG-PLA nano micelles with different drug loading capacities, and subjecting the nano micelles for freeze-drying for later use. The nano ginsenoside CK is prepared, the water solubility of the nano ginsenoside CK is improved, theefficacy of the nano ginsenoside CK is expected to be enhanced, the whitening effect of the nano ginsenoside CK is studied, and the nano ginsenoside CK is expected to be used as a nano ginseng cosmetic in the cosmetic fields of whitening, wrinkle resistance, freckle removal and the like.
Description
technical field [0001] The invention relates to the technical field of cosmetics, in particular to a preparation method of nanometer ginsenoside CK. Background technique [0002] Ginseng has a unique nourishing beauty effect. As a traditional Chinese beauty product, it is recognized as having the effects of whitening, anti-wrinkle, and anti-aging. Since the 1980s, ginseng has been widely used in cosmetics, such as ginseng moisturizer, Various ginseng cosmetics such as ginseng whitening cream and ginseng mask have become one of the most widely used plant cosmetic raw materials. [0003] The whitening effect is usually achieved by inhibiting the production of melanin. Skin whitening agents usually target tyrosinase, and reduce the production of melanin by inhibiting the activity of tyrosinase or blocking the oxidation pathway of tyrosine to produce melanin. , to achieve the effect of whitening the skin. [0004] Ginsenoside CK is a metabolite of diol-type ginsenosides in the...
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