Human pulmonary infection assessment method based on intestinal flora in excrement sample and application of human pulmonary infection assessment method

A technology for fecal samples and intestinal flora, applied in the field of biomedicine, to reduce patient pain, improve prognosis, and high detection efficiency

Pending Publication Date: 2021-02-09
中诺(杭州)基因科技有限责任公司
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At present, there is no set of methods to evaluate the degree of illness an...

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  • Human pulmonary infection assessment method based on intestinal flora in excrement sample and application of human pulmonary infection assessment method
  • Human pulmonary infection assessment method based on intestinal flora in excrement sample and application of human pulmonary infection assessment method
  • Human pulmonary infection assessment method based on intestinal flora in excrement sample and application of human pulmonary infection assessment method

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Embodiment Construction

[0026] A method for evaluating human lung infection based on intestinal flora in stool samples of the present invention comprises the following steps:

[0027] The first step is to collect the stool samples of the test personnel and extract DNA. Use the stool genomic DNA extraction kit (DP328) of Tiangen Biochemical Technology Co., Ltd. and extract the stool sample DNA as the template DNA according to the instructions. The selected test personnel include 20 ordinary patients with new coronary pneumonia, 19 severe patients and 17 critical patients were recorded as ordinary group, severe group and critical group respectively. The average age of 20 ordinary patients with new coronary pneumonia was 59 years old, and the age range was 53-63 years old. There were 8 males and 12 females; the average age of the 19 critically ill patients was 66 years old, and the age range was 61-74 years old, 9 males and 10 females; the average age of the 17 critically ill patients was 61 years old, a...

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Abstract

The invention discloses a human pulmonary infection assessment method based on intestinal flora in an excrement sample. The method comprises the following steps: collecting the excrement sample of a tested person and extracting DNA; configuring a PCR reaction system and performing PCR amplification; establishing a standard curve; performing quantitative analysis to obtain the quantity of the intestinal flora in the excrement sample; and calculating the ratio of enterococcus to faecalibacterium prausnitzii, and assessing the illness degree and prognosis condition of the tested person by combining the ratio with the value of the bifidobacterium content (Ec/Fp-B). According to the faecalibacterium prausnitzii method, the ratio of the enterococcus to the faecalibacterium prausnitzii in combination with the bifidobacterium content (Ec/Fp-B) can be used for assessing the disease degree and prognosis condition of pulmonary infection patients. The method has the advantages that the ratio of enterococcus to faecalibacterium prausnitzii in the intestinal flora is combined with bifidobacteria to effectively assess and judge the disease degree and prognosis condition of the pulmonary infectionpatients, the method is noninvasive and non-interventional, the pain of the patients is relieved, the detection efficiency is high, the detection cost is low and the like.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for evaluating human lung infection based on intestinal flora in stool samples and its application in patients with new coronary pneumonia infection. 【Background technique】 [0002] Infection refers to the local tissue and systemic inflammatory response caused by bacteria, viruses, fungi, parasites and other pathogens invading the human body, including blood infection, central nervous system infection, respiratory tract infection, digestive tract infection, urinary tract infection, etc. [0003] Our study found that patients with lung infection, especially severe and critically ill patients, have intestinal microecological imbalance. The gut is the largest metabolic and immune organ in the body. More and more evidence shows that the gut can participate in the regulation of various diseases through the "gut-lung axis", "gut-brain axis" and "gut-liver axi...

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/689C12Q1/6883C12Q1/06C12R1/01
CPCC12Q1/6851C12Q1/689C12Q1/6883C12Q2600/118C12Q2531/113C12Q2545/113C12Q2563/107
Inventor 李强汤灵玲曹丽萍徐威张辉马涛孙聪
Owner 中诺(杭州)基因科技有限责任公司
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