141 results about "Enterococcus sp" patented technology

The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.

The invention discloses a production method and an application of high-yield gamma-aminobutyric acid. The conservation number of high-yield gamma-aminobutyric acid (GABA) raffinose enterococcus M1 is CGMCC (China General Microbiological Culture Collection Center) No.5584. The production method comprises the following steps of: separating and selecting strains to obtain an Entercoccusraffinosus TCCC11660 (CGMCCNo. 5584) strain of high-yield gamma-aminobutyric acid; optimizing a fermentation culture medium and fermentation conditions; and carrying out a 10L fermentation tank experiment. The production method has the advantages of cheap raw materials, low production energy consumption, low production cost, good product safety and the like, and industrial production is easy to realize. The strain is applied to production of gamma-aminobutyric acid through glutamic acid fermentation and has good social and economic benefits.

The invention discloses microbial fermentation feed. The microbial fermentation feed is prepared from, by weight, 3-5 parts of a complex microbial agent, 680-730 parts of a fermentation raw material,275-325 parts of an energy raw material, 350-400 parts of water and 1-2 parts of brown sugar by mixed fermentation. The complex microbial agent is made by mixing of 5-6 of bacillus subtilis, enterococcus faecalis, plant lactobacillus, bacillus pumilus, clostridium butyricum, bacillus megaterium, bacillus amyloliquefaciens or lactobacillus casei. By fermentation treatment of the fermentation raw material and the energy raw material with the complex fermentation strain, enzymolysis of coarse fibers in the fermentation raw material is realized, organic nitrogen and inorganic nitrogen, which can be hardly used by animals, in the fermentation raw material are converted into bacterial protein, and macromolecular carbohydrates, which cannot be absorbed by the animal, in the energy raw material are converted into absorbable low-molecule carbohydrates.

The invention discloses a compound beta-mannase and lactobacillus feed additive. The feed additive comprises the following components in part by weight: beta-mannase 2-8, xylanase 24-30, beta-glucanase 9-15, cellulose 28-33, pectase 20-27 and Enterococcus faecium fermentation liquor 90-110. The invention also discloses an Enterococcus faecium strain EF1008. The feed additive of the invention can be added in feed for egg-laying chickens in a proportion of 0.03 to 0.1 percent. The feed additive can improve the egg laying rate of the egg-laying chickens and can be used at a high rate by animals and reduce the cost of the feed. In the feed additive, the Enterococcus faecium can promote the micro ecological balance in the intestinal tract, improve body immunity and reduce the death rate of livestock in a stressful environment.

The invention provides biological fermentation active carrier type composite premix feed as well as a preparation method and an application thereof. The preparation method comprises the following steps: preparing probiotics biological fermentation active carriers, to be specific, respectively carrying out liquid fermentation cultivation by using lactobacillus acidophilus LA0912, enterococcus faecalis EF1109, bacillus subtilis BS0903 and saccharomyces cerevisiae SC1203, mixing obtained liquid according to a volume ratio being 1: 1: 1: 1, then carrying out solid fermentation cultivation, drying an obtained solid fermentation culture at lower temperature and grinding so as to obtain the biological fermentation active carriers; compounding the biological fermentation active carriers with vitamins, amino acids, minerals, organic trace elements, choline chloride and table salt so as to obtain the biological fermentation active carrier type composite premix feed suitable for pigs at different stages. The biological fermentation active carrier type composite premix feed gives play to the health function of probiotics, makes the best of multiple biological activity effects of probiotics fermentation metabolites and can meet the physiologic nutritional requirements of pigs at different stages.

The invention relates to a traditional Chinese medicine microecological preparation for improving the immunity of chicks, and a preparation method and usage of the traditional Chinese medicine microecological preparation, belonging to the technical field of veterinary traditional Chinese medicine microecological preparations. The traditional Chinese medicine microecological preparation is prepared by carrying out mixed fermentation on fermentation strains and the following raw materials: 15-20 parts of radix astragali seu hedysari, 5-10 parts of Chinese angelica, 5-10 parts of fructus ligustri lucidi, 5-10 parts of radix isatidis, 5-10 parts of folium isatidis, 15-20 parts of cortex eucommiae, 5-10 parts of fructus crataegi, 5-10 parts of malt, 1-2 parts of brown sugar, 1-3 parts of fructo-oligosaccharide and 1-3 parts of lysine; the fermentation strains comprise a bacillus subtilis strain, a lactobacillus plantarum strain, an enterococcus faecium strain and saccharomyces cerevisiae. The traditional Chinese medicine microecological preparation can enhance the immunity of the chicks and improve the digestion-absorption function of the intestinal tracts of the chicks, thus achieving the aims of increasing the disease resistance of the chicks as well as reducing the morbidity and mortality of the chicks on the whole, and increasing the economic benefit.

The invention provides a method for producing gamma-aminobutyric acid by separated fermentation of an enterococcus faecium, which is characterized in that the enterococcus faecium is taken as a strain, the pH value and temperature condition of deep fermentation is controlled by step, and glutamic acid or sodium glutamate is supplemented externally, thus producing the gamma-aminobutyric acid by fermentation, wherein the concentration of the gamma-aminobutyric acid in a fermenting mash reaches 10-20g/L. The method has the advantages of simplifying the fermentation process, having high yield and utilization ratio of the equipment, convenient operation, and the like.

The invention provides feed for feeding a broiler, which comprises basic feed compositions, nicotinic acid, asparaginic acid, methionine, citric acid, malic acid, fish meal, vegetable oil, biotin, amylase, protease, manganese oxide, ferrous sulfate, wine brewing yeast, enterococcus faecium and a Chinese herbal medicine composition additive. The feed provided by the invention has the benefits thatthe immunocompetence of the broiler can be improved, and the probability that the broiler falls ill in the feeding process can be reduced. The feed has no drug residue, so that the survival rate of the broiler is increased.

The invention provides biological fermentation type functional feed for bighead carp. The feed is mainly prepared from components in parts by weight as follows: 30-70 parts of fish meal, 300-600 partsof distillers' grains, 200-400 parts of soybean meal, 50-100 parts of bran, 70-130 parts of rice bran and 15-35 parts of a premix. The premix comprises a fermenting agent and components capable of promoting bighead carp culture such as multi-vitamins for fish and compound ore elements for fish, and the fermenting agent comprises saccharomyces cerevisiae, lactobacillus plantarum, bacillus coagulans, enterococcus faecalis and clostridium butyricum. The invention also provides a preparation method of the biological fermentation type functional feed for bighead carp. The method mainly comprises the following steps: uniformly mixing all the components, directly placing the mixture in a net bag with a respiratory membrane, and fermenting the mixture for 7-10 days to obtain the feed. The biological fermentation type functional feed for bighead carp is simple to prepare, not high in equipment requirement, high in operability and easy to promote and use in large-scale farms or aquaculture cooperatives.

The invention relates to a liquid cleaning and disinfecting hand dishwashing detergent composition comprising at least one surfactant selected from the group consisting of anionic, nonionic, cationic, zwitterionic, amphoteric surfactants, and mixtures thereof, at least one organic solvent and/or at least one hydrotrope, and a single antibacterial active and/or at least one sequestering agent. The cleaning and disinfecting hand dishwashing detergent composition eliminates 99.999% of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus hirae in no more than 5 minutes at an 80% product concentration and 20 degrees Celsius according to the EN1276 suspension test

The present invention further relates to methods of cleaning and disinfecting dishware and/or dishwashing implements and/or skin using such a liquid of cleaning and disinfecting detergent composition.

The invention relates to a phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials and a preparation method thereof. The phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials is prepared by fermenting the following components in percentage by weight:8-15% of bean pulp, 2-10% of corn protein, 40-60% of bran, 1.0-2.0% of Bacillus subtilis, 1.0-2.0% of Lactobacillus acidophilus, 1.0-2.0% of Enterococcus faecalis, 1.0-2.0% of microzyme, 0.1-0.5% part of brown sugar, 0.02-0.08% of yeast extract, 0.02-0.08% of potassium dihydrogen phosphate, 0.02-0.08% of neutral proteinase, 0.02-0.08% of acid protease and 20-30% of water. The invention has the advantages of environmental protection, healthiness, favorable phagostimulant effect, strong capacity for killing pathogenic bacteria, and favorable therapeutic effect for porcine anorexia.

The invention discloses a preparation method and application of a fermented Chinese herb biological fodder for ruminant animals. The Chinese herb biological fodder is prepared from the following raw materials of 21 to 29 parts of radix astragali seu hedysari, 18 to 32 parts of poria cocos, 22 to 28 parts of radix codonopsis, 9 to 18 parts of wormwood and 10 to 20 parts of liquorice root; aspergillus oryzae, bacillus natto and phaffia rhodozyma are inoculated for aerobic fermentation, and then enterococcus faecium and pediococcus pentosaceus are inoculated for anaerobic fermentation to obtain the fermented Chinese herb biological fodder. According to the fermented Chinese herb biological fodder disclosed by the invention, Chinese herb raw material compatibility is reasonable; by means of step-by-step fermentation of beneficial microorganisms, pharmaceutical functions of the Chinese herbs are obviously improved; meanwhile, a lot of metabolite is generated, the rumen environment of the ruminant animals is improved, the fodder utilization rate is improved, diseases are reduced, the fodder cost is reduced, and the economical benefits are improved.

The present invention relates to a dibenzoanthracene oxepanone compound from a marine fungus WH4-2 and a preparation method and application thereof. The marine fungus talaromyces stipitatus has the efficient V.parahemolyticus activity, the yield of an active substance, namely the dibenzoanthracene oxepanone compound containing an isoprene group is large, the dibenzoanthracene oxepanone compound isexpected to be applied to medicinal development of novel V.parahemolyticus, it is found that the compound further has Bacillus subtilis, Staphylococcus aureus ATCC29213 and Enterococcus faecium ATCC35667 resistance activity in further activity analysis, and the dibenzoanthracene oxepanone compound is expected to be developed into a broad-spectrum antibacterial medicine.

The invention provides a peach gum polysaccharide degradation product PGP-1.The peach gum polysaccharide degradation product PGP-1, with molecular weight between 1X10<5> and 1X10<6>, contains no neutral polysaccharide and consists of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 0.637:0.054:0.073:0.022:0.556.The peach gum polysaccharide degradation product PGP-1 is simple to prepare and capable of promoting bifidobacterium growth remarkably, inhibiting enterococcus faecium growth remarkably, scavenging hydroxyl free radicals and DPPH free radicals effectively and inhibiting Hela cell growth remarkably; Galectin-3-mediated cell agglutination inhibiting experiments show that the peach gum polysaccharide degradation product PGP-1 is capable of remarkably inhibiting agglutination of chicken blood red blood cells, Hela cells and MCF-7 cells mediated by Galectin-3.

The invention provides a preparation method of a fermented protein feed and the fermented protein feed, and belongs to the technical field of biological fermented feeds. The preparation method comprises the following steps: 1) mixing a protein raw material with water, Tween 80 and a peroxyacetic acid solution to obtain a mixed material; 2) inoculating the mixed material obtained in the step 1) with aspergillus oryzae, and performing solid-state aerobic fermentation to obtain a fermented material; and 3) mixing the fermented material obtained in the step 2) with an enterococcus faecium culturesolution and water, and performing anaerobic fermentation and enzymolysis to obtain the fermented protein feed. Experimental results show that the preparation method of the fermented protein feed hasthe effects of reducing the contents of toxic, harmful and anti-nutritional ingredients in protein raw materials, increasing the content of acid-soluble protein, increasing the solubility of protein,improving and regulating amino acid balance and improving the palatability of the feed, so that the nutritional requirements for production of complete formula feeds or breeding of animals can be better met.