93 results about "Enterococcus sp" patented technology

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The present application describes in part isolated and modified Enterococci toxin (Epx) polypeptides, immunogenic compositions comprising Exp polypeptides, nanopores formed by Epx polypeptides, apparatus comprising Epx nanopores, and methods of use thereof.

The invention provides a microbial composition and application thereof in preparation of selenium-rich sleep-aiding goat milk powder, and belongs to the technical field of microbial fermentation. The microbial composition disclosed by the invention comprises bifidobacterium animalis, enterococcus durans and candida tropicalis. A food additive obtained by fermenting Chinese yam, selenium-rich black beans, Chinese wolfberry fruits, licorice roots, wheat and tremella fuciformis through the microbial composition has the sleep aiding effect, milk powder prepared by combining the microbial composition with other raw materials has the remarkable sleep improving effect, and the milk powder can be taken for a long time and is safe, nutritional and healthy.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention provides an antibacterial protein targeting chicken enterococcus as well as a preparation method and application of the antibacterial protein. Specifically, the invention provides an antibacterial protein targeting chicken enterococcus or a chimeric compound thereof, and the antibacterial protein comprises one or more of proteins formed by the following amino acid sequences: (a) an amino acid sequence as shown in any one of SEQ ID NO: 1 to SEQ ID NO: 9; (b) a derivative sequence which is obtained by replacing, adding and / or deleting one or more amino acids from the amino acid sequence of (a) and has the same function as (a); and (c) a derived amino acid sequence of which the identity with any one sequence from SEQ ID NO: 1 to SEQ ID NO: 9 is 85% or more or 90% or more. The antibacterial protein disclosed by the invention can be used for efficiently killing chicken enterococcus and has specificity.

The invention discloses a fermented Chinese herbal medicine preparation for improving eggshell quality and a preparation method of the fermented Chinese herbal medicine preparation, Chinese herbal medicines mainly comprise motherwort, folium artemisiae argyi, radix isatidis and jujube powder, and fermentation probiotics mainly comprise bacillus subtilis, bacillus coagulans, enterococcus faecalis, lactobacillus plantarum and saccharomycetes. The fermented traditional Chinese medicine preparation produced by the invention has the effects of improving intestinal health and immunity and improving oxidation resistance and stress resistance of laying hens in laying hen production, so that the egg quality and eggshell quality of the laying hens are improved.

The invention discloses a preparation process and application of enterococcus hirsutei I DO5 freeze-dried powder. The preparation process has the following beneficial effects that (1) compared with the prior art, the preparation process of the enterococcus hirsutei I DO5 freeze-dried powder disclosed by the invention is simpler and easier to operate when being used for preparing enterococcus hirsutei I DO5 into immobilized pellets; (2) the enterococcus hirsutei I DO5 is prepared into freeze-dried powder, and the physiological activity of the enterococcus hirsutei I DO5 strain can be better reserved at low temperature and low pressure in the freeze-drying process; the enterococcus hirsuti I DO5 freeze-dried powder can be stably stored for a long time, so that the requirement of frequent supplement is reduced, and the enterococcus hirsuti I DO5 freeze-dried powder is more suitable for a long-term use breeding scene (3) the enterococcus hirsutei I DO5 freeze-dried powder is good in deodorization effect, and the degradation rates of indole, skatole and ammonia gas in the pig manure wastewater can reach 60% or above; and (4) the surface morphology of the enterococcus hisei I DO5 freeze-dried powder can be effectively improved through the protective agent solution, the freezing resistance of cells is enhanced, the integrity of a cell membrane structure is maintained, and the freeze-drying survival rate of the enterococcus hisei I DO5 is increased.

The invention relates to the technical field of extraction and utilization of chemical substances in walnut green seedcases, in particular to a production process of urolithin A. The production process specifically comprises the following steps: obtaining the walnut green seedcases; walnut green seedcase is mashed into pulp, a stabilizing solution is added, ultrasonic treatment is performed after uniform stirring, the fermentation substrate is obtained, and the stabilizing solution is an aqueous solution of a compound of sulfydryl-beta-cyclodextrin and chitosan; primary fermentation: inoculating a microbial bacterial liquid A for anaerobic fermentation, then filtering and collecting filtrate, the microbial bacterial liquid A being obtained by respectively inoculating bacillus velezensis and bacillus coagulans into a culture medium A for shake cultivation and activation and then mixing the bacillus velezensis and the bacillus coagulans; secondary fermentation: inoculating the filtrate with a microbial bacterial liquid B for anaerobic fermentation again, wherein the microbial bacterial liquid B is obtained by inoculating enterococcus faecium into a culture medium B for culture; and extracting. According to the method, the stabilizing solution is added into the walnut green seedcase, and two-stage fermentation is performed, so that the yield and the conversion rate of urolithin A are remarkably improved, and the method is safe, environmentally friendly and capable of being industrialized.

The invention belongs to the technical field of target sample characterization, and provides application of enterococcus pore-forming toxin mutant protein in nanopore detection. The enterococcus pore-forming toxin mutant protein is EPX1 nanopore mutant protein and is obtained by mutating wild type EPX1 nanopore protein, the amino acid sequence of the wild type EPX1 nanopore protein is as shown in SEQ ID NO: 1, and the mutation means that one or more amino acids of the wild type EPX1 nanopore protein are mutated into common amino acids except original amino acids. The EPX1 protein (wild type) is a type of pore-forming toxin protein and widely exists in various living bodies in nature, and a mutant of the EPX1 protein shows excellent pore-forming capacity after being modified (after amino acid mutation). The structural stability, the molecular recognition efficiency and the detection performance of the EPX1 nanopore mutant protein are remarkably improved.

The invention discloses a compound microecological preparation, fermentation liquor and application of the compound microecological preparation in laying hen production. The compound micro-ecological preparation is prepared from clostridium butyricum NIP1021, bacillus cereus 2022B1, bacillus licheniformis 2022B2 and enterococcus faecium 2022B6. The fermentation liquor of the compound micro-ecological preparation can promote the growth performance levels of the Haillan grey laying hens, such as the final weight and the like; the development of thymus and bursa of Fabricius immune organs of laying hens can be promoted; the content of serum immune globulin IgG and serum-related immune cell factors IL-1beta, IL-6 and TNF-alpha of laying hens is remarkably increased, so that the serum nonspecific immune level of animal bodies is improved; according to the method, the intestinal villus area of the laying hens is increased, the nutrient absorption capacity of the laying hens is improved, cecum flora diversity and species abundance are increased, dominant flora categories are thick-wall flora and bacteroides to regulate intestinal flora balance, and the method has positive significance in maintaining intestinal health.

The invention discloses a uric acid-reducing and gout-resisting composition as well as a preparation method and application thereof. The composition capable of reducing uric acid and resisting gout comprises oligosaccharide, plant powder, yeast powder, oligopeptide and probiotic powder. The plant powder in the composition has a diuretic effect and can promote purine and uric acid to be discharged along with urine, so that the content of the uric acid is reduced; the yeast powder is introduced to promote dissolution and discharge of purine, so that the content of uric acid is reduced; probiotics are introduced, reproduction of harmful bacteria such as escherichia coli, clostridium perfringens and enterococcus is inhibited, proliferation of the probiotics is promoted, the micro-ecological environment of the intestinal tract is improved, uric acid entering the intestinal tract is decomposed through metabolism, dissolution of the uric acid is promoted, the influence of intestinal flora on uric acid excretion is reduced, and it is ensured that uric acid in the intestinal tract is excreted; the uric acid reducing and gout resisting composition disclosed by the invention has a relatively good protection effect on the liver.

The invention discloses a biological deodorant for a garbage station and a preparation method of the biological deodorant, and belongs to the technical field of biological deodorization, the biological deodorant is prepared by mixing carefully screened saccharomyces cerevisiae, bacillus amyloliquefaciens, enterococcus faecalis and lactobacillus plantarum to form a compound microbial community, and culturing to obtain a compound microbial inoculum stock solution. The microbial carrier is prepared by combining natural materials such as kaolin, zeolite and diatomite with chemical synthetic materials such as polyether polyol and isocyanate, and carrying out high-temperature roasting and alkali liquor treatment to form the carrier with high adsorbability and stability. And finally, immersing the sterilized microbial carrier into the complex microbial inoculant stock solution to prepare the efficient and stable biological deodorant. Compared with the prior art, the biological deodorant prepared by the method shows excellent deodorization effect and long-term stability in a simulated garbage station environment, and a new solution is provided for stink control of the garbage station and other environments.

The invention discloses a modified chitosan compound with a function of promoting healing of seawater soaking wounds. The modified chitosan compound DGCS prepared by the invention has good biological activity, has a remarkable promotion effect on cell proliferation, and is enhanced along with the increase of concentration; the inflammation level in cells can be reduced; the healing of a seawater soaking wound can be promoted. An antibacterial experiment shows that the DGCS has good antibacterial activity on escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa, enterococcus faecalis, vibrio vulnificus and vibrio parahaemolyticus.

Described herein are compounds and compositions for use in treatment or prevention of an inflammatory bowel disease, gastrointestinal cancer, or a systemic bacterial infection in a subject in need thereof. They subject may be colonized by one or more pathogenic bacterial strains such as B. fragilis, E. faecalis, or C. perfringens. In certain aspects, the disclosure provides a method of diminishing the pathogenic effects of these bacterial stmins by administering a compound that binds to and / or inhibits one or more toxins produced thereby.

The invention discloses a humic acid type straw source microbial agent which comprises the following strains: enterococcus faecium, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The method has the advantages that turfy soil, biogas residues, biogas slurry and a certain amount of agricultural waste are added into compost with straw microbial agents and livestock and poultry manure as main materials to adjust a proper carbon nitrogen ratio, and more importantly, a compound microbial agent for strengthening the process of the humic acid type straw source microbial agent is inoculated; compared with natural composting fermentation, the composting efficiency and stability are improved, and the fertilizer efficiency and fertility of the organic fertilizer and the resource utilization efficiency of waste are improved.

The invention discloses application of ocean-derived enterococcus durans YQ-6 in improving the growth performance, slaughter performance and muscle quality of Leizhou black ducks, and belongs to the technical field of poultry breeding. Experiments prove that the marine-derived enterococcus durans YQ-6 can improve the growth performance and muscle quality of the Leizhou black ducks, enhance the immune function and oxidation resistance of organisms, improve the intestinal flora structure of the Leizhou black ducks and improve the intestinal health, and the effect is optimal when 1 * 10 < 9 > CFU / kg of the marine-derived enterococcus durans YQ-6 is added into feed. The feed disclosed by the invention complies with the development trend of'feed resistance-free ', and has important significance on green, safe and sustainable development of the breeding industry.

The invention relates to the technical field of feed additives, in particular to a sustained-release composition for solving ruminant rumen acidosis as well as a preparation method and application of the sustained-release composition. The composition is prepared from the following components in parts by weight: 30 to 60 parts of complex microbial inoculant capsules, 10 to 30 parts of thiamine, 5 to 15 parts of light calcium carbonate, 5 to 15 parts of magnesium oxide, 8 to 18 parts of sodium bicarbonate, 2 to 5 parts of rare earth elements, 3 to 8 parts of rhizoma coptidis, 5 to 15 parts of rhizoma atractylodis macrocephalae, 4 to 9 parts of poria cocos and 2 to 5 parts of rhizoma corydalis. The complex microbial inoculant capsule at least comprises parabacteroides dieldrii, bacillus subtilis, bacillus licheniformis, enterococcus faecalis and lactobacillus rosenbergii; the rare earth element is a mixture of lanthanum nitrate and cerous nitrate, and the mass ratio of lanthanum nitrate to cerous nitrate is 1: 1. The composition can be used for preventing and treating ruminant rumen acidosis, can be slowly released in the stomach due to the slow release effect, and does not need to be used as a feed additive all the time.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

The invention provides a natto food antibacterial preservative, a preparation method and application, and belongs to the technical field of food preservation, the preservative is mainly prepared from the following raw materials: mixed beans, protease, bacillus natto and enterococcus faecium; wherein the mixed beans consist of the following components in parts by mass: 30-50 parts of soybeans, 40-50 parts of peas, 5-10 parts of oats and 10-20 parts of wheat; the use amount of the protease is 0.1-0.5% of the total mass of the mixed beans; the use amount of the bacillus natto is 1%-3% of the total mass of the mixed beans; the dosage of the enterococcus faecium is 1%-3% of the total mass of the mixed beans. Through scientific compatibility, the antibacterial and preservative effects of the natto paste are improved, and the technical blank of organic natto preservatives in the field of long-acting bacteriostasis of food bodies is filled.

The invention provides a feed additive for improving transportation stress reaction of ruminants as well as a preparation method and application of the feed additive, and relates to the technical field of functional feed additives. The feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material, the probiotic composition is composed of bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, and the traditional Chinese medicine fermented material is composed of codonopsis pilosula, fructus forsythiae, radix bupleuri, soybean meal and bran. The feed additive can influence the physiological indexes, biochemical indexes and blood indexes of the ruminants and promote the indexes to recover to the level before transportation, so that the transportation stress response of the ruminants is greatly improved.

The invention belongs to the technical field of environmental biology, and particularly relates to a key gene for degrading skatole by enterococcus haiseri ID05 and application of the key gene for degrading skatole by enterococcus haiseri ID05, and the test process of the key gene for degrading skatole by enterococcus haiseri ID05 is as follows: selecting an enterococcus haiseri IDO5 strain, an LB culture medium, an inorganic salt culture medium and reagent consumables and instruments required in the test process; the degradation rate of the enterococcus haiseri IDO5 on 100 mg / L of skatole in an inorganic salt culture medium reaches 67.01 + / -0.01%, and it is shown that the enterococcus haiseri IDO5 has the good degradation capacity on the skatole. Besides, the degradation rate of the IDO5 on skatole in sterilized pig manure wastewater reaches up to 96.10%, the degradation rate of the IDO5 on skatole in unsterilized pig manure wastewater is also 78.17%, and the enterococcus hirsuti IDO5 has good degradation capacity on skatole in pig manure wastewater and has good application potential in pig manure wastewater, so that when the pig manure wastewater is discharged, the enterococcus hirsuti IDO5 can be used for degrading the skatole in the pig manure wastewater. After being treated by enterococcus hirsuti IDO5, the wastewater can be discharged, so that the pollution to the environment is effectively reduced.

This invention relates to the field of biotechnology, specifically disclosing a freeze-drying protection system for Enterococcus faecalis, its preparation method, and its applications. The freeze-drying protection system comprises arginine, skim milk, and glycerol, combined with low-temperature stress pretreatment. The freeze-drying protection system provided by this invention significantly increases the freeze-drying survival rate of Enterococcus faecalis L118 to 87.90±1.28%, and the survival rate remains above 90% after 28 days of storage at -20℃. The core probiotic characteristics of the freeze-dried Enterococcus faecalis L118 strain, such as acid production capacity, simulated gastric juice, and NaCl tolerance, are well preserved, providing an effective solution for preparing highly active and stable Enterococcus faecalis preparations.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention provides a harmless treatment method and application of chicken manure, and relates to the technical field of waste recycling. Specifically, the method comprises an ozone oxidation process, a drying process, an enzymolysis process, an aerobic fermentation process and an anaerobic fermentation process which are carried out in sequence, wherein in the aerobic fermentation process, a pre-product is mixed with charcoal, a cellulose source, plant ash, wine grape skin and rice bran meal for fermentation; in the anaerobic fermentation process, compound bacteria including clostridium butyricum, pediococcus acidilactici, bacillus coagulans and enterococcus faecium are added into a pre-product, and sealed fermentation is performed. The chicken manure is subjected to targeted detoxification treatment according to the characteristics of the chicken manure, pathogenic microorganisms and complex heavy metals in the chicken manure can be effectively removed, the content of mycotoxin in the chicken manure is reduced, nutrient substances in the chicken manure are decomposed, resource application in the field of fertilizer or feed is achieved, and the method has good application prospects.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The invention provides a fermented traditional Chinese medicine feed additive for sheep and a preparation method thereof, and relates to the technical field of fermented traditional Chinese medicine feed additives. The feed additive is prepared from composite probiotic powder consisting of bacillus subtilis, lactobacillus plantarum and enterococcus faecalis, and a traditional Chinese medicine composition consisting of gorgon fruit, fructus psoraleae, codonopsis pilosula, bighead atractylodes rhizome, common carpesium fruit, antifebrile dichroa, campsis grandiflora, butterflybush flower and liquorice. The feed additive disclosed by the invention can effectively improve the growth performance and the immunity of sheep, has a remarkable improvement effect on meat quality, and can realize the effects of effectively preventing parasites and promoting healing at the same time.

This invention discloses two ferulic acid esterase-producing strains isolated from baijiu daqu (fermentation starter) makers: *Enterococcus faecalis* L-7 and *Burkholderia burgdorferi* BK100. These strains are deposited at the China Center for Type Culture Collection (CCTCC), with accession numbers CCTCC NO.M 20221555 and CCTCC NO.M 20221553, respectively. Optimization of the fermentation conditions for *Enterococcus faecalis* L-7 revealed that the optimal fermentation conditions were: 32℃, 160 r / min, and an inoculum size of 6%, for 96 h. During this period, the strain exhibited the highest ferulic acid esterase activity, reaching 44.68 U / L, a 68.92% increase compared to the unoptimized 26.45 U / L. *Burkholderia burgdorferi* BK100, characterized by high ferulic acid esterase activity and high-temperature resistance, is suitable for daqu preparation and baijiu brewing, and can increase ferulic acid content and improve baijiu quality.

The present invention provides a strain of Bacillus Velez G14 and its application. The strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 16, 2024, with a deposit number of GDMCC NO: 64871, and the deposit address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The Bacillus Velez G14 strain and its fermentation broth of the present invention can not only effectively inhibit Pasteurella multocida, Enterobacter aerogenes, Enterococcus faecalis and Pseudomonas aeruginosa, but also have better genetic stability, acid-base stability, temperature stability, enzyme stability, ultraviolet stability and storage stability.

A kind of fermentation processing method and production equipment system of Gongfu black tea, the Gongfu black tea is formed tea mixture by adding lophatherum gracile, dried tangerine or orange peel and jasmine petals in tea leaves, withering, fermentation, shaping and drying, the fermentation includes pre-fermentation and complete fermentation, wherein pre-fermentation is the self-oxidation fermentation of tea leaves, complete fermentation is microbial fermentation, specifically, first step fermentation is carried out by using acetic acid bacteria and enterococcus faecalis to form compound microbial inoculant, then abnormal hansenula is added to carry out second step fermentation.In the present application, the differences in raw material fermentation process of each component during fermentation are overcome, which leads to the problems of sour and stale taste, etc.in tea leaves.The types and content of aroma substances are increased, and the problem of insufficient infusion resistance of loose tea is solved.The Gongfu black tea prepared by the present application has red and bright golden ring tea soup color, the ratio of teaflavins, theabrownins and thearubigins is coordinated, so that the tea soup is sweet and smooth with honey aroma when entering the mouth, and the aroma is long-lasting in cold cup.

The present invention discloses a preparation process and application of Enterococcus hirae I DO5 freeze-dried powder, which has the following beneficial effects: (1) the preparation process of Enterococcus hirae I DO5 freeze-dried powder of the present invention is simpler and easier to operate than the existing preparation process of preparing Enterococcus hirae I DO5 into immobilized pellets in the prior art; (2) by preparing Enterococcus hirae I DO5 into the form of freeze-dried powder, the low temperature and low pressure during the freeze-drying process can better retain the physiological activity of the Enterococcus hirae I DO5 strain; the Enterococcus hirae I DO5 freeze-dried powder can be stably stored for a long time, reducing the need for frequent replenishment, and is more suitable for long-term use in breeding scenarios; (3) the Enterococcus hirae I DO5 freeze-dried powder has a good deodorizing effect, and the degradation rate of indole, skatole and ammonia in pig manure wastewater can reach more than 60%; (4) the protective agent solution can effectively improve the surface morphology of the Enterococcus hirae I DO5 freeze-dried powder, enhance the cell's anti-freezing ability, maintain the integrity of the cell membrane structure, and improve the freeze-dried survival rate of Enterococcus hirae I DO5.