141 results about "Enterococcus sp" patented technology

The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.

The invention discloses a wheat middling-corn compound feed for weaned pigs. The wheat middling-corn compound feed comprises the following components in part by weight: 15-17 parts of corn, 8-10 parts of broken rice, 45-50 parts of wheat middling, 4-4.5 parts of biscuit powder, 5-6 parts of wheat bran, 8-10 parts of soybean meal, 2-2.5 parts of chocolate, 1-2 parts of fish powder, and 0.15-0.2 part of composite microorganism bacterium agent, wherein the composite microorganism bacterium agent comprises one or more of enterococcus, saccharomycete and bacillus. The compound feed reduces the content of the corn and the soybean pump by using the wheat middling as a substitute, so that the production cost of the feed is greatly reduced. The wheat-middling compound feed further contains composite microorganism bacterium agent, so that the weaned pigs can well digest the wheat middling, and the nutritions needed for the growth and development of the weaned pigs are completely satisfied; the growth performance is remarkably improved relative to the corn-soybean pulp daily ration; moreover, diarrhea incidence rate is not improved, but reduced, thereby providing a good basis for post high-speed growth of the weaned pigs.

The invention discloses a production method and an application of high-yield gamma-aminobutyric acid. The conservation number of high-yield gamma-aminobutyric acid (GABA) raffinose enterococcus M1 is CGMCC (China General Microbiological Culture Collection Center) No.5584. The production method comprises the following steps of: separating and selecting strains to obtain an Entercoccusraffinosus TCCC11660 (CGMCCNo. 5584) strain of high-yield gamma-aminobutyric acid; optimizing a fermentation culture medium and fermentation conditions; and carrying out a 10L fermentation tank experiment. The production method has the advantages of cheap raw materials, low production energy consumption, low production cost, good product safety and the like, and industrial production is easy to realize. The strain is applied to production of gamma-aminobutyric acid through glutamic acid fermentation and has good social and economic benefits.

The invention discloses microbial fermentation feed. The microbial fermentation feed is prepared from, by weight, 3-5 parts of a complex microbial agent, 680-730 parts of a fermentation raw material,275-325 parts of an energy raw material, 350-400 parts of water and 1-2 parts of brown sugar by mixed fermentation. The complex microbial agent is made by mixing of 5-6 of bacillus subtilis, enterococcus faecalis, plant lactobacillus, bacillus pumilus, clostridium butyricum, bacillus megaterium, bacillus amyloliquefaciens or lactobacillus casei. By fermentation treatment of the fermentation raw material and the energy raw material with the complex fermentation strain, enzymolysis of coarse fibers in the fermentation raw material is realized, organic nitrogen and inorganic nitrogen, which can be hardly used by animals, in the fermentation raw material are converted into bacterial protein, and macromolecular carbohydrates, which cannot be absorbed by the animal, in the energy raw material are converted into absorbable low-molecule carbohydrates.

The invention discloses a digestible creep feed for sucking piglets. The digestible creep feed comprises the following ingredients based on parts by weight: 15.5-16.5 parts of corn, 22-25 parts of broken rice, 7-9 parts of soybean meal, 5-6.5 parts of imported fish meal, 1.5-2.5 parts of wheat bran, 1.8-2.5 parts of chocolate, 1.8-2.3 parts of cane sugar, 11-15 parts of low protein whey powder, 7-9 parts of wheat shorts, 4.5-5.5 parts of biscuit powder, 1.8-2.2 parts of soybean oil and 0.06-0.1 parts of complex microbial community; wherein, the complex microbial community comprises one or more of the followings: enterococcus, saccharomycetes and bacillus. Corn and soybean meal are reduced in the creep feed and replaced by the wheat shorts, and thus the production cost of the creep feed is greatly reduced. The creep feed also comprises complex microbial community so that the wheat shorts digestion of the sucking piglets is improved. Therefore, the nutrients required by the growth of the sucking piglets can be fully satisfied. The creep feed is developed according to the intestinal development and feeding habits of the sucking piglets and has a better effect of creep feeding.

The invention provides newly separated enterococcus faecium (enterococcus faecium) HEW-A588. The enterococcus faecium HEW-A588 has obvious probiotic and stress resistance. When the micro-ecologic preparation prepared from the enterococcus faecium enters intestinal tracts of animals, the micro-ecologic preparation is rapidly activated and proliferated, the micro-ecologic preparation can have the efficacies of forming beneficial advantage microbial populations, maintaining the balance of the intestinal tracts, improving the food utilization efficiency of the animals, reducing the cost, improving the steady state of the internal environment of the digestive tracts in the animal bodies, promoting the absorption and the utilization of nutrition, promoting the growth of the animals, improving economic benefits and the like, and the micro-ecologic preparation has favorable application prospects.

The invention discloses a compound beta-mannase and lactobacillus feed additive. The feed additive comprises the following components in part by weight: beta-mannase 2-8, xylanase 24-30, beta-glucanase 9-15, cellulose 28-33, pectase 20-27 and Enterococcus faecium fermentation liquor 90-110. The invention also discloses an Enterococcus faecium strain EF1008. The feed additive of the invention can be added in feed for egg-laying chickens in a proportion of 0.03 to 0.1 percent. The feed additive can improve the egg laying rate of the egg-laying chickens and can be used at a high rate by animals and reduce the cost of the feed. In the feed additive, the Enterococcus faecium can promote the micro ecological balance in the intestinal tract, improve body immunity and reduce the death rate of livestock in a stressful environment.

The invention provides biological fermentation active carrier type composite premix feed as well as a preparation method and an application thereof. The preparation method comprises the following steps: preparing probiotics biological fermentation active carriers, to be specific, respectively carrying out liquid fermentation cultivation by using lactobacillus acidophilus LA0912, enterococcus faecalis EF1109, bacillus subtilis BS0903 and saccharomyces cerevisiae SC1203, mixing obtained liquid according to a volume ratio being 1: 1: 1: 1, then carrying out solid fermentation cultivation, drying an obtained solid fermentation culture at lower temperature and grinding so as to obtain the biological fermentation active carriers; compounding the biological fermentation active carriers with vitamins, amino acids, minerals, organic trace elements, choline chloride and table salt so as to obtain the biological fermentation active carrier type composite premix feed suitable for pigs at different stages. The biological fermentation active carrier type composite premix feed gives play to the health function of probiotics, makes the best of multiple biological activity effects of probiotics fermentation metabolites and can meet the physiologic nutritional requirements of pigs at different stages.

The invention discloses a feed additive premix capable of reducing the excrement stink of broilers and application of the feed additive premix. The feed additive premix comprises the following components: bacillus licheniformis, bacillus subtilis, clostridium butyricum, enterococcus faecium, lactobacillus reuteri, a yucca extract, a camphor extract, fructooligosaccharide and rice protein peptide. The feed additive premix provided by the invention does not contain any antibiotic or drug residue and is an environment-friendly and pollution-free high-quality feed additive premix for broilers. The feed additive premix has the advantages of being free of toxic and side effects, being beneficial to growth of broilers and human health, and being simple and convenient to prepare and use. The feed additive premix has biological functions of promoting the growth and the intestinal health of broilers, improving the immune functions of organisms, and the like. The feed additive premix has the effects of effectively reducing stink gas including ammonia gas, hydrogen sulfide and the like produced by broilers, improving the growth environment of broilers, and reducing infection rates of diseases including respiratory diseases, intestine diseases and the like and the death rate of broilers.

The invention relates to a traditional Chinese medicine microecological preparation for improving the immunity of chicks, and a preparation method and usage of the traditional Chinese medicine microecological preparation, belonging to the technical field of veterinary traditional Chinese medicine microecological preparations. The traditional Chinese medicine microecological preparation is prepared by carrying out mixed fermentation on fermentation strains and the following raw materials: 15-20 parts of radix astragali seu hedysari, 5-10 parts of Chinese angelica, 5-10 parts of fructus ligustri lucidi, 5-10 parts of radix isatidis, 5-10 parts of folium isatidis, 15-20 parts of cortex eucommiae, 5-10 parts of fructus crataegi, 5-10 parts of malt, 1-2 parts of brown sugar, 1-3 parts of fructo-oligosaccharide and 1-3 parts of lysine; the fermentation strains comprise a bacillus subtilis strain, a lactobacillus plantarum strain, an enterococcus faecium strain and saccharomyces cerevisiae. The traditional Chinese medicine microecological preparation can enhance the immunity of the chicks and improve the digestion-absorption function of the intestinal tracts of the chicks, thus achieving the aims of increasing the disease resistance of the chicks as well as reducing the morbidity and mortality of the chicks on the whole, and increasing the economic benefit.

The invention provides a method for producing gamma-aminobutyric acid by separated fermentation of an enterococcus faecium, which is characterized in that the enterococcus faecium is taken as a strain, the pH value and temperature condition of deep fermentation is controlled by step, and glutamic acid or sodium glutamate is supplemented externally, thus producing the gamma-aminobutyric acid by fermentation, wherein the concentration of the gamma-aminobutyric acid in a fermenting mash reaches 10-20g / L. The method has the advantages of simplifying the fermentation process, having high yield and utilization ratio of the equipment, convenient operation, and the like.

The invention discloses a large Enterococcus faecium strain that could be used as feed additive for domestic animal.

The invention provides feed for feeding a broiler, which comprises basic feed compositions, nicotinic acid, asparaginic acid, methionine, citric acid, malic acid, fish meal, vegetable oil, biotin, amylase, protease, manganese oxide, ferrous sulfate, wine brewing yeast, enterococcus faecium and a Chinese herbal medicine composition additive. The feed provided by the invention has the benefits thatthe immunocompetence of the broiler can be improved, and the probability that the broiler falls ill in the feeding process can be reduced. The feed has no drug residue, so that the survival rate of the broiler is increased.

The invention relates to a feeding attracting type fermented feed and a preparation method thereof. The feeding attracting type fermented feed is prepared by fermenting the following constituents by weight percent: 8%-40% of one substance or a mixture of two substances selected from bean pulp and corn gluten meal, 30%-70% of bran, 1.0%-2.0% of bacillus subtilis, 1.0%-2.0% of lactobacillus acidophilus, 1.0%-2.0% of enterococcus faecalis, 1.0%-2.0% of saccharomycetes, 0.1%-0.5% of brown sugar, 0.02%-0.08% of yeast extract, 0.02%-0.08% of monopotassium phosphate, 0.02%-0.08% of neutral protease, 0.02%-0.08% of acid proteinase and 15%-30% of water. The feeding attracting type fermented feed has the advantages of nontoxicity, harmlessness, environment friendliness and healthiness, is rich in a lot of lactic acid, excellent in feeding attracting effect and strong in sterilization capability, and has better treatment effect on anorexia of pigs.

The invention provides biological fermentation type functional feed for bighead carp. The feed is mainly prepared from components in parts by weight as follows: 30-70 parts of fish meal, 300-600 partsof distillers' grains, 200-400 parts of soybean meal, 50-100 parts of bran, 70-130 parts of rice bran and 15-35 parts of a premix. The premix comprises a fermenting agent and components capable of promoting bighead carp culture such as multi-vitamins for fish and compound ore elements for fish, and the fermenting agent comprises saccharomyces cerevisiae, lactobacillus plantarum, bacillus coagulans, enterococcus faecalis and clostridium butyricum. The invention also provides a preparation method of the biological fermentation type functional feed for bighead carp. The method mainly comprises the following steps: uniformly mixing all the components, directly placing the mixture in a net bag with a respiratory membrane, and fermenting the mixture for 7-10 days to obtain the feed. The biological fermentation type functional feed for bighead carp is simple to prepare, not high in equipment requirement, high in operability and easy to promote and use in large-scale farms or aquaculture cooperatives.

The invention relates to a liquid cleaning and disinfecting hand dishwashing detergent composition comprising at least one surfactant selected from the group consisting of anionic, nonionic, cationic, zwitterionic, amphoteric surfactants, and mixtures thereof, at least one organic solvent and / or at least one hydrotrope, and a single antibacterial active and / or at least one sequestering agent. The cleaning and disinfecting hand dishwashing detergent composition eliminates 99.999% of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus hirae in no more than 5 minutes at an 80% product concentration and 20 degrees Celsius according to the EN1276 suspension testThe present invention further relates to methods of cleaning and disinfecting dishware and / or dishwashing implements and / or skin using such a liquid of cleaning and disinfecting detergent composition.

The invention relates to a phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials and a preparation method thereof. The phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials is prepared by fermenting the following components in percentage by weight:8-15% of bean pulp, 2-10% of corn protein, 40-60% of bran, 1.0-2.0% of Bacillus subtilis, 1.0-2.0% of Lactobacillus acidophilus, 1.0-2.0% of Enterococcus faecalis, 1.0-2.0% of microzyme, 0.1-0.5% part of brown sugar, 0.02-0.08% of yeast extract, 0.02-0.08% of potassium dihydrogen phosphate, 0.02-0.08% of neutral proteinase, 0.02-0.08% of acid protease and 20-30% of water. The invention has the advantages of environmental protection, healthiness, favorable phagostimulant effect, strong capacity for killing pathogenic bacteria, and favorable therapeutic effect for porcine anorexia.

The invention discloses a preparation method and application of a fermented Chinese herb biological fodder for ruminant animals. The Chinese herb biological fodder is prepared from the following raw materials of 21 to 29 parts of radix astragali seu hedysari, 18 to 32 parts of poria cocos, 22 to 28 parts of radix codonopsis, 9 to 18 parts of wormwood and 10 to 20 parts of liquorice root; aspergillus oryzae, bacillus natto and phaffia rhodozyma are inoculated for aerobic fermentation, and then enterococcus faecium and pediococcus pentosaceus are inoculated for anaerobic fermentation to obtain the fermented Chinese herb biological fodder. According to the fermented Chinese herb biological fodder disclosed by the invention, Chinese herb raw material compatibility is reasonable; by means of step-by-step fermentation of beneficial microorganisms, pharmaceutical functions of the Chinese herbs are obviously improved; meanwhile, a lot of metabolite is generated, the rumen environment of the ruminant animals is improved, the fodder utilization rate is improved, diseases are reduced, the fodder cost is reduced, and the economical benefits are improved.

The invention discloses an aflatoxin B1 control compound biological agent and application thereof. The aflatoxin B1 control compound biological agent is formed by compounding bacillus subtilis, bacillus licheniformis, enterococcus faecium and yeast cell wall, wherein the bacillus subtilis, the bacillus licheniformis and the enterococcus faecium can effectively inhibit growth of mould in feeds. Theaflatoxin B1 control compound biological agent effectively combines two feed-contained aflatoxin B1 detoxification methods, namely, biodegradation and bio-absorption; the contained bacillus subtiliscan effectively degrade aflatoxin B1, and the contained yeast cell wall can effectively absorb aflatoxin B1. Besides, the aflatoxin B1 control compound biological agent can not only achieve mould preventing and cleaning functions, but also achieve microbial healthcare effects.

The invention discloses a microbial flora additive used for a sheep feed. The microbial flora additive is characterized by comprising the components of complex microbial floras, bifidobacterium bifidum, brewer's yeast, bacillus coagulans, bacillus subtilis and enterococcus faecalis according to the ratio of (56-58):(24-27):7:7:1, wherein the viable count of the complex microbial floras is between 2.0*10<8>cfu / g and 1.0*10<9>cfu / g. The microbial floras regulate the micro-ecological balance in the intestinal tract through the collaborative action, the use of medicines like antibiotics is avoided, the product is more environmentally friendly, the cost is low, and the microbial flora additive has an excellent application prospect.

The invention provides a fermented feed for fish. The fermented feed is prepared through performing mixed fermentation on raw materials, composite microbes A, composite microbes B and water, wherein the composite microbes A comprise one or more of bacillus subtilis, bacillus licheniformis, bacillus coagulans and candida utilis; and the composite microbes B comprise one or more of lactobacillus acidophilus, plant lactic acid bacteria and enterococcus faecalis. According to the fermented feed for fish, provided by the invention, a two-step fermentation method is adopted, the composite microbes Aare used for performing aerobic fermentation for 2-3 days at an early stage, the composite microbes B are added at a later stage, airtight fermentation is performed for 5-7 days, and the synergisticreaction of different strains is used for degrading macromolecular protein in the raw materials and reducing the content of anti-nutritional factors; besides, a large number of bioactive substances are produced, and the fermented feed which is high in nutrient value and easy to digest and utilize is obtained; and then other feed raw materials are added, a cold granulating making technology is usedfor cooperation, and the fermented cold granulated feed is prepared, so that active beneficial factors in the fermentation process are reserved to the maximum extent.

The present invention relates to novel phenyl oxazolidinone compounds of formula I, their pharmaceutically acceptable analogs, tautomeric forms, stereoisomers, polymorphs, prodrugs, metabolites, salts or solvates thereof. The invention also relates to the processes for the synthesis of novel compounds of formula I or their pharmaceutically acceptable analogs, tautomeric forms, stereoisomers, polymorphs, prodrugs, metabolites, salts or solvates thereof. The present invention also provides pharmaceutical compositions comprising novel compounds of formula I and methods of using them. The compounds of the present invention are useful as antimicrobial agents, effective against a number of aerobic and / or anaerobic Gram positive and / or Gram negative pathogens such as multi drug resistant species of Staphylococcus, Streptococcus, Enterococcus, Bacterioides, Clostridia, H. influenza, Moraxella, acid-fast organisms such as Mycobacterium tuberculosis as well as Linezolid resistant species of Staphylococcus and Enterococcus.

The present invention relates to a dibenzoanthracene oxepanone compound from a marine fungus WH4-2 and a preparation method and application thereof. The marine fungus talaromyces stipitatus has the efficient V.parahemolyticus activity, the yield of an active substance, namely the dibenzoanthracene oxepanone compound containing an isoprene group is large, the dibenzoanthracene oxepanone compound isexpected to be applied to medicinal development of novel V.parahemolyticus, it is found that the compound further has Bacillus subtilis, Staphylococcus aureus ATCC29213 and Enterococcus faecium ATCC35667 resistance activity in further activity analysis, and the dibenzoanthracene oxepanone compound is expected to be developed into a broad-spectrum antibacterial medicine.

The invention provides a peach gum polysaccharide degradation product PGP-1.The peach gum polysaccharide degradation product PGP-1, with molecular weight between 1X10<5> and 1X10<6>, contains no neutral polysaccharide and consists of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 0.637:0.054:0.073:0.022:0.556.The peach gum polysaccharide degradation product PGP-1 is simple to prepare and capable of promoting bifidobacterium growth remarkably, inhibiting enterococcus faecium growth remarkably, scavenging hydroxyl free radicals and DPPH free radicals effectively and inhibiting Hela cell growth remarkably; Galectin-3-mediated cell agglutination inhibiting experiments show that the peach gum polysaccharide degradation product PGP-1 is capable of remarkably inhibiting agglutination of chicken blood red blood cells, Hela cells and MCF-7 cells mediated by Galectin-3.

The invention discloses a loop mediated isothermal amplification detection primer group, a detection method and a kit for enterococcus faecalis. The detection primer group is as follows: an upstream outer primer is F3:5'-GCCATTCCTCATGGAACAG-3'; a downstream outer primer is B 3:5'-CCACGTTATCCATATCACTACA-3'; an upstream inner primer is FIP:5'-CGGTGCCAAAATTAACACCCTTTAGTGAAAAAATCAGGAATCTGTG-3'; a downstream inner primer is BIP:5'-TGCTACCGTATTATTTGGGATTGCAAAGTGCAATTTGTTGGACTA-3'. The detection method has the advantages of high flexibility, strong specificity, high accuracy, short detection time. From sample treatment to report only takes 12 hours, a PCR (polymerase chain reaction) and an electrophoresis apparatus do not need, the operation process is simple, compared with other PCR technology, the detection method has higher specificity, is applicable to self-detection of basic level detection organizations and drinking water processors.

The invention provides a peach gum polysaccharide degradation product PGP-2. The PGP-2 is obtained by obtaining a fermentation broth by inoculating a peach gum culture medium with microbacterium A5 and performing separation and purification; the microbacterium A5 is preserved, with the preservation number of CCTCC NO:M209174 in the CCTCC (China Center for Type Culture Collection) on August 7, 2009; anion exchange is adopted for separation and purification, and a NaCl solution is adopted for gradient elution. The PGP-2 is simple to prepare, can significantly promote growth of Bifidobacterium while inhibiting growth of Enterococcus faecium, has higher scavenging activity for hydroxyl free radicals and DPPH free radicals and can significantly inhibit growth and proliferation of Hela cells, Galectin-3 mediated cell agglutination experiments indicate that the PGP-2 can significantly inhibit agglutination of Galectin-3 mediated red blood cells, Hela cells and MCF-7 cells.

The invention relates to a feed additive composition and a preparation method thereof. The feed additive composition is composed of isomalto-oligosaccharide, fructo-oligosaccharide, radix astragali polysaccharide, bacillus subtilis, bacillus licheniformis, plant lactobacillus, lactobacillus acidophilus, enterococcus faecium, yeast-zinc, yeast-selenium, glucosylase, an anti-caking agent, and a carrier. The formula is scientific, the cost of signal component is high, the effect is bad, and the provided formula solves the problems. The cost is effectively reduced, while at the same time, an optimal effect is achieved. The composition can be added into feed to effectively exert the healthcare functions, can be used to replace antibiotics, and is safe to eat.

The invention discloses a fermented feed protein as well as a preparation method and application thereof. The preparation method of the fermented feed protein disclosed by the invention utilizes a stepwise fermentation way which specifically comprises the following steps: fermenting moringa seed meal with aspergillus niger and rhizopus oryzae so as to have macromolecules, including cellulose, proteins and the like, degraded into small molecules; inoculating the fermented moringa seed meal with enterococcus faecium so as to allow direct use of small-molecule substances; and then, further fermenting the moringa seed meal so as to have fermentation efficiency improved, thereby more effectively increasing nutritional values of the moringa seed meal. Being subjected to the fermentation, the moringa seed meal is capable of producing sour and aromatic flavor; and moreover, the fermented moringa seed meal has significantly improved crude protein content, acid-soluble protein content, neutral protease activity and the like compared with moringa seed meal subjected to single-bacterium fermentation. Therefore, the fermented moringa seed meal is rich in probiotics, and becomes a high-quality feed protein.

The invention provides a preparation method of a fermented protein feed and the fermented protein feed, and belongs to the technical field of biological fermented feeds. The preparation method comprises the following steps: 1) mixing a protein raw material with water, Tween 80 and a peroxyacetic acid solution to obtain a mixed material; 2) inoculating the mixed material obtained in the step 1) with aspergillus oryzae, and performing solid-state aerobic fermentation to obtain a fermented material; and 3) mixing the fermented material obtained in the step 2) with an enterococcus faecium culturesolution and water, and performing anaerobic fermentation and enzymolysis to obtain the fermented protein feed. Experimental results show that the preparation method of the fermented protein feed hasthe effects of reducing the contents of toxic, harmful and anti-nutritional ingredients in protein raw materials, increasing the content of acid-soluble protein, increasing the solubility of protein,improving and regulating amino acid balance and improving the palatability of the feed, so that the nutritional requirements for production of complete formula feeds or breeding of animals can be better met.