77 results about "Enterococcus sp" patented technology

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The present application describes in part isolated and modified Enterococci toxin (Epx) polypeptides, immunogenic compositions comprising Exp polypeptides, nanopores formed by Epx polypeptides, apparatus comprising Epx nanopores, and methods of use thereof.

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention discloses a fermented Chinese herbal medicine preparation for improving eggshell quality and a preparation method of the fermented Chinese herbal medicine preparation, Chinese herbal medicines mainly comprise motherwort, folium artemisiae argyi, radix isatidis and jujube powder, and fermentation probiotics mainly comprise bacillus subtilis, bacillus coagulans, enterococcus faecalis, lactobacillus plantarum and saccharomycetes. The fermented traditional Chinese medicine preparation produced by the invention has the effects of improving intestinal health and immunity and improving oxidation resistance and stress resistance of laying hens in laying hen production, so that the egg quality and eggshell quality of the laying hens are improved.

The invention discloses a preparation process and application of enterococcus hirsutei I DO5 freeze-dried powder. The preparation process has the following beneficial effects that (1) compared with the prior art, the preparation process of the enterococcus hirsutei I DO5 freeze-dried powder disclosed by the invention is simpler and easier to operate when being used for preparing enterococcus hirsutei I DO5 into immobilized pellets; (2) the enterococcus hirsutei I DO5 is prepared into freeze-dried powder, and the physiological activity of the enterococcus hirsutei I DO5 strain can be better reserved at low temperature and low pressure in the freeze-drying process; the enterococcus hirsuti I DO5 freeze-dried powder can be stably stored for a long time, so that the requirement of frequent supplement is reduced, and the enterococcus hirsuti I DO5 freeze-dried powder is more suitable for a long-term use breeding scene (3) the enterococcus hirsutei I DO5 freeze-dried powder is good in deodorization effect, and the degradation rates of indole, skatole and ammonia gas in the pig manure wastewater can reach 60% or above; and (4) the surface morphology of the enterococcus hisei I DO5 freeze-dried powder can be effectively improved through the protective agent solution, the freezing resistance of cells is enhanced, the integrity of a cell membrane structure is maintained, and the freeze-drying survival rate of the enterococcus hisei I DO5 is increased.

The invention belongs to the technical field of target sample characterization, and provides application of enterococcus pore-forming toxin mutant protein in nanopore detection. The enterococcus pore-forming toxin mutant protein is EPX1 nanopore mutant protein and is obtained by mutating wild type EPX1 nanopore protein, the amino acid sequence of the wild type EPX1 nanopore protein is as shown in SEQ ID NO: 1, and the mutation means that one or more amino acids of the wild type EPX1 nanopore protein are mutated into common amino acids except original amino acids. The EPX1 protein (wild type) is a type of pore-forming toxin protein and widely exists in various living bodies in nature, and a mutant of the EPX1 protein shows excellent pore-forming capacity after being modified (after amino acid mutation). The structural stability, the molecular recognition efficiency and the detection performance of the EPX1 nanopore mutant protein are remarkably improved.

The invention discloses a modified chitosan compound with a function of promoting healing of seawater soaking wounds. The modified chitosan compound DGCS prepared by the invention has good biological activity, has a remarkable promotion effect on cell proliferation, and is enhanced along with the increase of concentration; the inflammation level in cells can be reduced; the healing of a seawater soaking wound can be promoted. An antibacterial experiment shows that the DGCS has good antibacterial activity on escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa, enterococcus faecalis, vibrio vulnificus and vibrio parahaemolyticus.

Described herein are compounds and compositions for use in treatment or prevention of an inflammatory bowel disease, gastrointestinal cancer, or a systemic bacterial infection in a subject in need thereof. They subject may be colonized by one or more pathogenic bacterial strains such as B. fragilis, E. faecalis, or C. perfringens. In certain aspects, the disclosure provides a method of diminishing the pathogenic effects of these bacterial stmins by administering a compound that binds to and / or inhibits one or more toxins produced thereby.

The invention discloses a humic acid type straw source microbial agent which comprises the following strains: enterococcus faecium, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The method has the advantages that turfy soil, biogas residues, biogas slurry and a certain amount of agricultural waste are added into compost with straw microbial agents and livestock and poultry manure as main materials to adjust a proper carbon nitrogen ratio, and more importantly, a compound microbial agent for strengthening the process of the humic acid type straw source microbial agent is inoculated; compared with natural composting fermentation, the composting efficiency and stability are improved, and the fertilizer efficiency and fertility of the organic fertilizer and the resource utilization efficiency of waste are improved.

The invention discloses application of ocean-derived enterococcus durans YQ-6 in improving the growth performance, slaughter performance and muscle quality of Leizhou black ducks, and belongs to the technical field of poultry breeding. Experiments prove that the marine-derived enterococcus durans YQ-6 can improve the growth performance and muscle quality of the Leizhou black ducks, enhance the immune function and oxidation resistance of organisms, improve the intestinal flora structure of the Leizhou black ducks and improve the intestinal health, and the effect is optimal when 1 * 10 < 9 > CFU / kg of the marine-derived enterococcus durans YQ-6 is added into feed. The feed disclosed by the invention complies with the development trend of'feed resistance-free ', and has important significance on green, safe and sustainable development of the breeding industry.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

The invention provides a natto food antibacterial preservative, a preparation method and application, and belongs to the technical field of food preservation, the preservative is mainly prepared from the following raw materials: mixed beans, protease, bacillus natto and enterococcus faecium; wherein the mixed beans consist of the following components in parts by mass: 30-50 parts of soybeans, 40-50 parts of peas, 5-10 parts of oats and 10-20 parts of wheat; the use amount of the protease is 0.1-0.5% of the total mass of the mixed beans; the use amount of the bacillus natto is 1%-3% of the total mass of the mixed beans; the dosage of the enterococcus faecium is 1%-3% of the total mass of the mixed beans. Through scientific compatibility, the antibacterial and preservative effects of the natto paste are improved, and the technical blank of organic natto preservatives in the field of long-acting bacteriostasis of food bodies is filled.

The invention provides a feed additive for improving transportation stress reaction of ruminants as well as a preparation method and application of the feed additive, and relates to the technical field of functional feed additives. The feed additive comprises a probiotic composition and a traditional Chinese medicine fermented material, the probiotic composition is composed of bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, and the traditional Chinese medicine fermented material is composed of codonopsis pilosula, fructus forsythiae, radix bupleuri, soybean meal and bran. The feed additive can influence the physiological indexes, biochemical indexes and blood indexes of the ruminants and promote the indexes to recover to the level before transportation, so that the transportation stress response of the ruminants is greatly improved.

The invention belongs to the technical field of environmental biology, and particularly relates to a key gene for degrading skatole by enterococcus haiseri ID05 and application of the key gene for degrading skatole by enterococcus haiseri ID05, and the test process of the key gene for degrading skatole by enterococcus haiseri ID05 is as follows: selecting an enterococcus haiseri IDO5 strain, an LB culture medium, an inorganic salt culture medium and reagent consumables and instruments required in the test process; the degradation rate of the enterococcus haiseri IDO5 on 100 mg / L of skatole in an inorganic salt culture medium reaches 67.01 + / -0.01%, and it is shown that the enterococcus haiseri IDO5 has the good degradation capacity on the skatole. Besides, the degradation rate of the IDO5 on skatole in sterilized pig manure wastewater reaches up to 96.10%, the degradation rate of the IDO5 on skatole in unsterilized pig manure wastewater is also 78.17%, and the enterococcus hirsuti IDO5 has good degradation capacity on skatole in pig manure wastewater and has good application potential in pig manure wastewater, so that when the pig manure wastewater is discharged, the enterococcus hirsuti IDO5 can be used for degrading the skatole in the pig manure wastewater. After being treated by enterococcus hirsuti IDO5, the wastewater can be discharged, so that the pollution to the environment is effectively reduced.

This invention relates to the field of biotechnology, specifically disclosing a freeze-drying protection system for Enterococcus faecalis, its preparation method, and its applications. The freeze-drying protection system comprises arginine, skim milk, and glycerol, combined with low-temperature stress pretreatment. The freeze-drying protection system provided by this invention significantly increases the freeze-drying survival rate of Enterococcus faecalis L118 to 87.90±1.28%, and the survival rate remains above 90% after 28 days of storage at -20℃. The core probiotic characteristics of the freeze-dried Enterococcus faecalis L118 strain, such as acid production capacity, simulated gastric juice, and NaCl tolerance, are well preserved, providing an effective solution for preparing highly active and stable Enterococcus faecalis preparations.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention provides a harmless treatment method and application of chicken manure, and relates to the technical field of waste recycling. Specifically, the method comprises an ozone oxidation process, a drying process, an enzymolysis process, an aerobic fermentation process and an anaerobic fermentation process which are carried out in sequence, wherein in the aerobic fermentation process, a pre-product is mixed with charcoal, a cellulose source, plant ash, wine grape skin and rice bran meal for fermentation; in the anaerobic fermentation process, compound bacteria including clostridium butyricum, pediococcus acidilactici, bacillus coagulans and enterococcus faecium are added into a pre-product, and sealed fermentation is performed. The chicken manure is subjected to targeted detoxification treatment according to the characteristics of the chicken manure, pathogenic microorganisms and complex heavy metals in the chicken manure can be effectively removed, the content of mycotoxin in the chicken manure is reduced, nutrient substances in the chicken manure are decomposed, resource application in the field of fertilizer or feed is achieved, and the method has good application prospects.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

The invention provides a fermented traditional Chinese medicine feed additive for sheep and a preparation method thereof, and relates to the technical field of fermented traditional Chinese medicine feed additives. The feed additive is prepared from composite probiotic powder consisting of bacillus subtilis, lactobacillus plantarum and enterococcus faecalis, and a traditional Chinese medicine composition consisting of gorgon fruit, fructus psoraleae, codonopsis pilosula, bighead atractylodes rhizome, common carpesium fruit, antifebrile dichroa, campsis grandiflora, butterflybush flower and liquorice. The feed additive disclosed by the invention can effectively improve the growth performance and the immunity of sheep, has a remarkable improvement effect on meat quality, and can realize the effects of effectively preventing parasites and promoting healing at the same time.

This invention discloses two ferulic acid esterase-producing strains isolated from baijiu daqu (fermentation starter) makers: *Enterococcus faecalis* L-7 and *Burkholderia burgdorferi* BK100. These strains are deposited at the China Center for Type Culture Collection (CCTCC), with accession numbers CCTCC NO.M 20221555 and CCTCC NO.M 20221553, respectively. Optimization of the fermentation conditions for *Enterococcus faecalis* L-7 revealed that the optimal fermentation conditions were: 32℃, 160 r / min, and an inoculum size of 6%, for 96 h. During this period, the strain exhibited the highest ferulic acid esterase activity, reaching 44.68 U / L, a 68.92% increase compared to the unoptimized 26.45 U / L. *Burkholderia burgdorferi* BK100, characterized by high ferulic acid esterase activity and high-temperature resistance, is suitable for daqu preparation and baijiu brewing, and can increase ferulic acid content and improve baijiu quality.

The present invention provides a strain of Bacillus Velez G14 and its application. The strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 16, 2024, with a deposit number of GDMCC NO: 64871, and the deposit address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The Bacillus Velez G14 strain and its fermentation broth of the present invention can not only effectively inhibit Pasteurella multocida, Enterobacter aerogenes, Enterococcus faecalis and Pseudomonas aeruginosa, but also have better genetic stability, acid-base stability, temperature stability, enzyme stability, ultraviolet stability and storage stability.

A kind of fermentation processing method and production equipment system of Gongfu black tea, the Gongfu black tea is formed tea mixture by adding lophatherum gracile, dried tangerine or orange peel and jasmine petals in tea leaves, withering, fermentation, shaping and drying, the fermentation includes pre-fermentation and complete fermentation, wherein pre-fermentation is the self-oxidation fermentation of tea leaves, complete fermentation is microbial fermentation, specifically, first step fermentation is carried out by using acetic acid bacteria and enterococcus faecalis to form compound microbial inoculant, then abnormal hansenula is added to carry out second step fermentation.In the present application, the differences in raw material fermentation process of each component during fermentation are overcome, which leads to the problems of sour and stale taste, etc.in tea leaves.The types and content of aroma substances are increased, and the problem of insufficient infusion resistance of loose tea is solved.The Gongfu black tea prepared by the present application has red and bright golden ring tea soup color, the ratio of teaflavins, theabrownins and thearubigins is coordinated, so that the tea soup is sweet and smooth with honey aroma when entering the mouth, and the aroma is long-lasting in cold cup.

The present invention discloses a preparation process and application of Enterococcus hirae I DO5 freeze-dried powder, which has the following beneficial effects: (1) the preparation process of Enterococcus hirae I DO5 freeze-dried powder of the present invention is simpler and easier to operate than the existing preparation process of preparing Enterococcus hirae I DO5 into immobilized pellets in the prior art; (2) by preparing Enterococcus hirae I DO5 into the form of freeze-dried powder, the low temperature and low pressure during the freeze-drying process can better retain the physiological activity of the Enterococcus hirae I DO5 strain; the Enterococcus hirae I DO5 freeze-dried powder can be stably stored for a long time, reducing the need for frequent replenishment, and is more suitable for long-term use in breeding scenarios; (3) the Enterococcus hirae I DO5 freeze-dried powder has a good deodorizing effect, and the degradation rate of indole, skatole and ammonia in pig manure wastewater can reach more than 60%; (4) the protective agent solution can effectively improve the surface morphology of the Enterococcus hirae I DO5 freeze-dried powder, enhance the cell's anti-freezing ability, maintain the integrity of the cell membrane structure, and improve the freeze-dried survival rate of Enterococcus hirae I DO5.

This invention discloses an anti-porcine reproductive and respiratory syndrome virus (PRRSV) preparation and its preparation method. The anti-PRRSV preparation comprises traditional Chinese medicine, excipients, and probiotics. The traditional Chinese medicine includes: mustard greens, thistle, bitter melon, and barley sprouts. The excipients include: soybean meal, wheat bran, and molasses. The probiotics include: Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae. By feeding pigs suffering from PRRSV with the anti-PRRSV preparation of this invention, PRRSV can be antagonized, improving the recovery rate of PRRSV-affected pigs and reducing the mortality rate. The anti-PRRSV preparation of this invention can be used as an antagonist of PRRSV. Furthermore, the anti-PRRSV preparation of this invention can also be used as a feed additive for the prevention or treatment of PRRSV in pigs.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The application discloses a kind of compound probiotic propagation methods and application, including the following steps: S1, in culture solution inoculation clostridium butyricum species carries out first fermentation, obtains first fermentation liquor;S2, inoculation lactobacillus plantarum species and Enterococcus faecium species carry out second fermentation, obtain second fermentation liquor;S3, inoculation yeast fungus species carries out third fermentation, obtains compound probiotic propagation liquor.The compound probiotic propagation liquor obtained in the application is added to pig feed for feeding, which can significantly improve the production performance of different pig groups, improve the skin redness and hair gloss of pig groups, increase the feed intake of pig groups, and reduce the breeding cost.

The invention discloses a novel anti-salmonella nano preparation CCJY as well as a preparation method and application thereof. The preparation comprises chitosan nano particles. The chitosan nanoparticle comprises cross-linked chitosan, and a lasso peptide MccJ25 and a lasso peptide MccY which are loaded on the chitosan. According to the invention, the synergistic antibacterial effect among different lasso peptides is exerted through a chemical cross-linking technology, on the basis of integrating the original antibacterial types of the lasso peptide MccJ25 and the lasso peptide MccY, the antibacterial types are expanded to serratia marcescens and enterococcus cloacae, and the broad-spectrum antibacterial effect is realized. Meanwhile, the invention further discloses a preparation method and application of the chitosan nano-particles and an anti-salmonella nano-preparation containing the chitosan nano-particles.

The invention relates to the technical field of feed additives, in particular to a feed additive for improving egg shell quality of laying hens and a preparation method thereof. The feed additive for improving the egg shell quality of the laying hens is prepared from the following components in parts by weight: 2 to 4 parts of taurine, 3 to 5 parts of radix angelicae sinensis, 3 to 5 parts of herba leonuri, 1 to 3 parts of iron glycinate, 2 to 3 parts of manganese glycinate, 0.5 to 1 part of zinc methionine, 0.1 to 0.4 part of selenium yeast, 0.1 to 0.2 part of 25-hydroxyD3, 3 to 5 parts of bile acid, 2 to 5 parts of enterococcus faecium, 1 to 2 parts of yeast cell wall polysaccharide, 2 parts of compound enzyme and 20 to 30 parts of montmorillonite. 40-50 parts of stone powder, 1 part of ethoxyquin and 10 parts of rice husk powder.

The invention belongs to the technical field of aquaculture, and particularly relates to a functional feed for improving immunity of eels and a preparation method of the functional feed. The feed comprises the following components in parts by mass: 5-8 parts of a microbial fermentation product of a mixture of soybean meal, rapeseed meal and cottonseed meal, 1-2 parts of a microbial fermentation product of a mixture of lentinus edodes stems and peanut meal, 0.5-1 part of fish meal, 2-3 parts of starch, 0.1-0.2 part of a functional additive of a mixture extract of folium cortex eucommiae and fructus piperis longi, and 0.05-0.1 part of enterococcus faecium. According to the eel feed disclosed by the invention, in combination with factors such as nutritional requirements and growth characteristics of eels, feed sources, environmental pollution degrees and the like, lentinus edodes stems and peanut meal fermentation products are added as natural amino acid additives of the feed, so that the growth performance of cultured fish can be improved, and a functional additive of folium cortex eucommiae and fructus piperis longi mixture extract is further introduced; through scientific compatibility, the feed cost is reduced, and the immunity promoting effect of the feed is optimized.

The present invention discloses a biological deodorant for garbage stations and a preparation method thereof, which belongs to the field of biological deodorization technology. The biological deodorant is formed by mixing carefully screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens, Enterococcus faecalis, and Lactobacillus plantarum to form a composite microbial community, and the composite bacterial agent stock solution is obtained after cultivation. The preparation of the microbial carrier adopts natural materials such as kaolin, zeolite, and diatomaceous earth, combined with chemical synthetic materials such as polyether polyols and isocyanates, and is subjected to high-temperature roasting and alkaline solution treatment to form a carrier with high adsorption and stability. Finally, the sterilized microbial carrier is immersed in the composite bacterial agent stock solution to prepare an efficient and stable biological deodorant. Compared with the existing technology, the biological deodorant prepared by this method shows excellent deodorization effect and long-term stability in a simulated garbage station environment, providing a new solution for odor control in environments such as garbage stations.