45 results about "Enterococcus sp" patented technology

The invention discloses plant probiotics with water retention, growth promotion and disease resistance effects and application thereof. The invention provides a complex microbial inoculant, which is prepared from the following active ingredients: 1) bacillus tequilensis Bac365 (GDMCC No: 65241), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) Enterococcus querebrasis Ent274 (GDMCC No: 65242) or Pseudomonas plecoglossa Pse350 (GDMCC No: 65243), and the active ingredients of the complex microbial inoculant are as follows: 1) bacillus tequilensis Bac365 (GDMCC No: 65243), 2) microbacterium azadirachta Mic250 (GDMCC No: 65244), and 3) the active ingredients of the complex microbial inoculant. The strain provided by the invention has good nitrogen fixation, phosphorus dissolution, IAA production, HA production and disease resistance, can effectively increase the utilization efficiency of water in drought soil, significantly promote plant growth and increase crop yield, and has good application prospects in the aspects of water retention and sand stabilization, agricultural production and the like.

The invention discloses a modified chitosan compound with a function of promoting healing of seawater soaking wounds. The modified chitosan compound DGCS prepared by the invention has good biological activity, has a remarkable promotion effect on cell proliferation, and is enhanced along with the increase of concentration; the inflammation level in cells can be reduced; the healing of a seawater soaking wound can be promoted. An antibacterial experiment shows that the DGCS has good antibacterial activity on escherichia coli, bacillus subtilis, staphylococcus aureus, pseudomonas aeruginosa, enterococcus faecalis, vibrio vulnificus and vibrio parahaemolyticus.

The invention discloses a humic acid type straw source microbial agent which comprises the following strains: enterococcus faecium, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The method has the advantages that turfy soil, biogas residues, biogas slurry and a certain amount of agricultural waste are added into compost with straw microbial agents and livestock and poultry manure as main materials to adjust a proper carbon nitrogen ratio, and more importantly, a compound microbial agent for strengthening the process of the humic acid type straw source microbial agent is inoculated; compared with natural composting fermentation, the composting efficiency and stability are improved, and the fertilizer efficiency and fertility of the organic fertilizer and the resource utilization efficiency of waste are improved.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

The invention provides a natto food antibacterial preservative, a preparation method and application, and belongs to the technical field of food preservation, the preservative is mainly prepared from the following raw materials: mixed beans, protease, bacillus natto and enterococcus faecium; wherein the mixed beans consist of the following components in parts by mass: 30-50 parts of soybeans, 40-50 parts of peas, 5-10 parts of oats and 10-20 parts of wheat; the use amount of the protease is 0.1-0.5% of the total mass of the mixed beans; the use amount of the bacillus natto is 1%-3% of the total mass of the mixed beans; the dosage of the enterococcus faecium is 1%-3% of the total mass of the mixed beans. Through scientific compatibility, the antibacterial and preservative effects of the natto paste are improved, and the technical blank of organic natto preservatives in the field of long-acting bacteriostasis of food bodies is filled.

The invention belongs to the technical field of environmental biology, and particularly relates to a key gene for degrading skatole by enterococcus haiseri ID05 and application of the key gene for degrading skatole by enterococcus haiseri ID05, and the test process of the key gene for degrading skatole by enterococcus haiseri ID05 is as follows: selecting an enterococcus haiseri IDO5 strain, an LB culture medium, an inorganic salt culture medium and reagent consumables and instruments required in the test process; the degradation rate of the enterococcus haiseri IDO5 on 100 mg / L of skatole in an inorganic salt culture medium reaches 67.01 + / -0.01%, and it is shown that the enterococcus haiseri IDO5 has the good degradation capacity on the skatole. Besides, the degradation rate of the IDO5 on skatole in sterilized pig manure wastewater reaches up to 96.10%, the degradation rate of the IDO5 on skatole in unsterilized pig manure wastewater is also 78.17%, and the enterococcus hirsuti IDO5 has good degradation capacity on skatole in pig manure wastewater and has good application potential in pig manure wastewater, so that when the pig manure wastewater is discharged, the enterococcus hirsuti IDO5 can be used for degrading the skatole in the pig manure wastewater. After being treated by enterococcus hirsuti IDO5, the wastewater can be discharged, so that the pollution to the environment is effectively reduced.

This invention relates to the field of biotechnology, specifically disclosing a freeze-drying protection system for Enterococcus faecalis, its preparation method, and its applications. The freeze-drying protection system comprises arginine, skim milk, and glycerol, combined with low-temperature stress pretreatment. The freeze-drying protection system provided by this invention significantly increases the freeze-drying survival rate of Enterococcus faecalis L118 to 87.90±1.28%, and the survival rate remains above 90% after 28 days of storage at -20℃. The core probiotic characteristics of the freeze-dried Enterococcus faecalis L118 strain, such as acid production capacity, simulated gastric juice, and NaCl tolerance, are well preserved, providing an effective solution for preparing highly active and stable Enterococcus faecalis preparations.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The application discloses a mesenchymal stem cell preparation and application thereof in thin endometrium repair, and comprises the following steps: adding Enterococcus durans CGMCC 1.3249 and Bacillus subtilis CGMCC 1.15792 supernatant as effective components into cultured mesenchymal stem cells; and the stem cell preparation obtained by the method can effectively restore the endometrial thickness of mice, increase the number of glands, and improve the pregnancy rate of the thin endometrium mice.

This invention discloses two ferulic acid esterase-producing strains isolated from baijiu daqu (fermentation starter) makers: *Enterococcus faecalis* L-7 and *Burkholderia burgdorferi* BK100. These strains are deposited at the China Center for Type Culture Collection (CCTCC), with accession numbers CCTCC NO.M 20221555 and CCTCC NO.M 20221553, respectively. Optimization of the fermentation conditions for *Enterococcus faecalis* L-7 revealed that the optimal fermentation conditions were: 32℃, 160 r / min, and an inoculum size of 6%, for 96 h. During this period, the strain exhibited the highest ferulic acid esterase activity, reaching 44.68 U / L, a 68.92% increase compared to the unoptimized 26.45 U / L. *Burkholderia burgdorferi* BK100, characterized by high ferulic acid esterase activity and high-temperature resistance, is suitable for daqu preparation and baijiu brewing, and can increase ferulic acid content and improve baijiu quality.

A kind of fermentation processing method and production equipment system of Gongfu black tea, the Gongfu black tea is formed tea mixture by adding lophatherum gracile, dried tangerine or orange peel and jasmine petals in tea leaves, withering, fermentation, shaping and drying, the fermentation includes pre-fermentation and complete fermentation, wherein pre-fermentation is the self-oxidation fermentation of tea leaves, complete fermentation is microbial fermentation, specifically, first step fermentation is carried out by using acetic acid bacteria and enterococcus faecalis to form compound microbial inoculant, then abnormal hansenula is added to carry out second step fermentation.In the present application, the differences in raw material fermentation process of each component during fermentation are overcome, which leads to the problems of sour and stale taste, etc.in tea leaves.The types and content of aroma substances are increased, and the problem of insufficient infusion resistance of loose tea is solved.The Gongfu black tea prepared by the present application has red and bright golden ring tea soup color, the ratio of teaflavins, theabrownins and thearubigins is coordinated, so that the tea soup is sweet and smooth with honey aroma when entering the mouth, and the aroma is long-lasting in cold cup.

This invention discloses an anti-porcine reproductive and respiratory syndrome virus (PRRSV) preparation and its preparation method. The anti-PRRSV preparation comprises traditional Chinese medicine, excipients, and probiotics. The traditional Chinese medicine includes: mustard greens, thistle, bitter melon, and barley sprouts. The excipients include: soybean meal, wheat bran, and molasses. The probiotics include: Bacillus subtilis, Enterococcus faecium, and Saccharomyces cerevisiae. By feeding pigs suffering from PRRSV with the anti-PRRSV preparation of this invention, PRRSV can be antagonized, improving the recovery rate of PRRSV-affected pigs and reducing the mortality rate. The anti-PRRSV preparation of this invention can be used as an antagonist of PRRSV. Furthermore, the anti-PRRSV preparation of this invention can also be used as a feed additive for the prevention or treatment of PRRSV in pigs.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

The application discloses a kind of compound probiotic propagation methods and application, including the following steps: S1, in culture solution inoculation clostridium butyricum species carries out first fermentation, obtains first fermentation liquor;S2, inoculation lactobacillus plantarum species and Enterococcus faecium species carry out second fermentation, obtain second fermentation liquor;S3, inoculation yeast fungus species carries out third fermentation, obtains compound probiotic propagation liquor.The compound probiotic propagation liquor obtained in the application is added to pig feed for feeding, which can significantly improve the production performance of different pig groups, improve the skin redness and hair gloss of pig groups, increase the feed intake of pig groups, and reduce the breeding cost.

The invention discloses a novel anti-salmonella nano preparation CCJY as well as a preparation method and application thereof. The preparation comprises chitosan nano particles. The chitosan nanoparticle comprises cross-linked chitosan, and a lasso peptide MccJ25 and a lasso peptide MccY which are loaded on the chitosan. According to the invention, the synergistic antibacterial effect among different lasso peptides is exerted through a chemical cross-linking technology, on the basis of integrating the original antibacterial types of the lasso peptide MccJ25 and the lasso peptide MccY, the antibacterial types are expanded to serratia marcescens and enterococcus cloacae, and the broad-spectrum antibacterial effect is realized. Meanwhile, the invention further discloses a preparation method and application of the chitosan nano-particles and an anti-salmonella nano-preparation containing the chitosan nano-particles.

The invention relates to the technical field of feed additives, in particular to a feed additive for improving egg shell quality of laying hens and a preparation method thereof. The feed additive for improving the egg shell quality of the laying hens is prepared from the following components in parts by weight: 2 to 4 parts of taurine, 3 to 5 parts of radix angelicae sinensis, 3 to 5 parts of herba leonuri, 1 to 3 parts of iron glycinate, 2 to 3 parts of manganese glycinate, 0.5 to 1 part of zinc methionine, 0.1 to 0.4 part of selenium yeast, 0.1 to 0.2 part of 25-hydroxyD3, 3 to 5 parts of bile acid, 2 to 5 parts of enterococcus faecium, 1 to 2 parts of yeast cell wall polysaccharide, 2 parts of compound enzyme and 20 to 30 parts of montmorillonite. 40-50 parts of stone powder, 1 part of ethoxyquin and 10 parts of rice husk powder.

This invention belongs to the field of livestock and poultry feed technology, specifically disclosing a high-efficiency compound feed for fattening pigs that resists disease and prevents diarrhea, and its preparation method. The feed includes corn, soybean meal, wheat bran, compound functional premix, vegetable oil, dicalcium phosphate, fish meal, salt, citric acid, maifanite, and zeolite. The compound functional premix contains ferrous sulfate, manganese sulfate, copper sulfate, calcium iodate, lysine, Enterococcus faecalis, and Bacillus subtilis. The Enterococcus faecalis and Bacillus subtilis are prepared using a multi-layer coating process. This invention utilizes the above-mentioned high-efficiency compound feed for fattening pigs that resists disease and prevents diarrhea, and its preparation method. This compound feed, through the scientific ratio of compound functional premix and basic feed, combined with innovative multi-layer coating technology and metal loading intercalation technology, achieves efficient colonization of probiotics and synergistic antibacterial effects, significantly improving the intestinal health and production performance of fattening pigs.

The invention relates to the field of treatment of obesity through intestinal bacteria transplantation, in particular to a marker combination related to the curative effect of treating obesity through flora transplantation, a product and application of the marker combination and the product. The biomarker composition is prepared from bifidobacterium animalis, ruminococcus sp, bifidobacterium adolescentis, a delazobivibrio sp, a delazobivibrio sp, a Eubacter asiaticus, an enterococcus faecalis and a Citrobacter amalonatus, and the biomarker composition is prepared from the following raw materials: the bifidobacterium animalis, the ruminococcus sp, the bifidobacterium adolescentis, the delazobivibrio sp, the delazobivibrio sp, the Eubacter asiaticus, the Enterococcus faecalis and the Citrobacter amalonatus. The invention provides a reagent for detecting the marker combination, which can be used for preparing a product for predicting / evaluating the curative effect, and also provides a computer readable storage medium containing a storage curative effect judgment instruction, and a curative effect evaluation system containing a detection device and a comparison device. The association between the flora and the obesity curative effect is disclosed for the first time, the method is non-invasive and high in accuracy, the blank that the flora transplantation treatment obesity curative effect lacks a specific marker is filled, and a basis is provided for obesity diagnosis and treatment and individualized treatment.

This invention belongs to the field of aquaculture, specifically relating to a composite nitrite degrading agent for aquaculture water and its preparation method. The method includes the following steps: modifying nano-iron with *Violetella foenum-graecum* extract; preparing the nitrite microecological degrading agent; and preparing the composite nitrite degrading agent. This invention uses a compound of *Bacillus belye*, *Glutamic acid bacillus mysore*, and *Enterococcus faecium* to construct the microecological degrading agent. Through the synergistic effect of nitrite reductase, the yield and specific activity of nitrite reductase are significantly improved, resulting in a nitrite degradation rate of much higher than that of a single-strain fermentation system, achieving highly efficient nitrite degradation.

The invention relates to bacillus velezensis B02 and application of the bacillus velezensis B02, and belongs to the technical field of microorganisms. The strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the strain is GDMCC NO.66439. The strain is obtained by separation, purification and screening from a soil sample, and tests show that bacteriostatic active substances generated by fermentation of the strain have good bacteriostatic effects on Burkholderia cepacia, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Pseudomonas putida, Enterococcus faecalis, Candida albicans and Aspergillus brazili. And the product also has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, and can be used for preparing daily chemical products. Therefore, the bacillus velezensis B02 and the bacteriostatic active substances generated by the bacillus velezensis B02 have wide application prospects and important transformation research values.

The invention relates to the technical field of bioengineering, in particular to a preparation method and application of a folium cortex eucommiae fermentation extract for promoting cartilage growth. According to the method, a four-stage dynamic regulation and control process of low-temperature plasma synergistic enzymolysis pretreatment, aerobic wall breaking, aerobic and anaerobic transition and anaerobic conversion is adopted; four specific strains with complementary functions, namely bacillus subtilis, aspergillus niger, saccharomyces cerevisiae and enterococcus faecalis, are selected, and precise temperature control and pH regulation are combined, so that efficient release and conversion of active ingredients of the folium cortex eucommiae are realized. The active ingredients of the extract are rich in variety and sufficient in content, cartilage cell proliferation and osteogenic differentiation can be remarkably promoted, and the extract has full-scene cartilage repair adaptability and is good in safety through toxicology verification. The preparation process is stable and repeatable, reaches the pilot test effect, can be adapted to various dosage forms such as oral preparations, injections and the like, and is suitable for preparing cartilage repair related medicines, functional foods and cartilage tissue engineering scaffold composite materials.

An immunomodulatory material and its preparation method. Said material comprises fermented rice bran and heat-killed Enterococcus faecalis, which concentration is not less than 5x 10 9 / g. Said material is prepared by cultivation of E. faecalis in a culture medium containing fermented rice bran and followed heat-sterilization.

This invention relates to the field of feed additive technology, and discloses a pet compound probiotic preparation rich in functional nutrients and its application. The pet compound probiotic preparation comprises the following components in parts by weight: compound matrix powder: 38.0-50.0 parts; Enterococcus faecium freeze-dried powder: 4.0-5.2 parts; Lactobacillus plantarum freeze-dried powder: 7.8-8.2 parts; trisodium citrate: 3.0-3.5 parts; L-glutamine: 16.5-20.0 parts; β-glucan: 12.4-15.0 parts; the compound matrix powder is made from sodium alginate 25.0-30.0 parts, betaine 5.0-8.0 parts, and calcium carbonate 8.0-12.0 parts. This invention's preparation can resist gastric acid and target intestinal release according to the digestive tract environment, effectively solving the problem of clumping when mixed with high-moisture food, and simultaneously achieving pet microecological regulation and damaged mucosal repair.

The application belongs to the technical field of characterizing target samples, and provides application of enterococcus pore-forming toxin mutant protein in nanopore detection. The enterococcus pore-forming toxin mutant protein is an EPX1 nanopore mutant protein, which is obtained by mutation of wild-type EPX1 nanopore protein, and the amino acid sequence of the wild-type EPX1 nanopore protein is shown as SEQ ID NO: 1. The mutation refers to that one or more amino acids of the wild-type EPX1 nanopore protein are mutated into common amino acids other than the original amino acids. The EPX1 protein (wild type) of the application is a kind of pore-forming toxin protein, which widely exists in various organisms in nature. The mutant thereof exhibits excellent pore-forming ability after modification (after amino acid mutation). The EPX1 nanopore mutant protein realizes significant improvement in structural stability, molecular recognition efficiency and detection performance.

The invention discloses enterococcus faecium for promoting intestinal tissue repair of aquatic animals and application of the enterococcus faecium, and relates to the technical field of aquaculture, the strain is named as GCE-2, the Latin name is Enterococcus faecium, the strain is preserved in the China General Microbiological Culture Collection Center on January 7, 2026, and the preservation number is CGMCC NO.37319. The invention further discloses a preparation method of the enterococcus faecium capable of promoting intestinal tissue repair of the aquatic animals and application of the enterococcus faecium capable of promoting the intestinal tissue repair of the aquatic animals. The enterococcus faecium GCE-2 provided by the invention can efficiently promote the repair of intestinal tissues of aquatic animals, has relatively strong intestinal colonization ability and good environmental adaptability, and can directly promote the regeneration of intestinal villus and the repair of epithelial cell injury, quickly relieve the pathological symptoms of intestinal tracts and remarkably improve the repair efficiency of the intestinal tissues.

This application discloses a method for the synergistic application of algae and bacteria in the treatment of livestock and poultry wastewater, relating to the field of livestock and poultry wastewater treatment technology. The application method includes the following steps: wastewater pretreatment; bacterial strain compounding: Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa are fermented individually, and then the single fermented bacterial strains are compounded. The mass ratio of the fermentation liquid of Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa is (1-3):1:(1-3):(3-5) to obtain a compound bacterial agent; microalgae compounding: Chlorella ellipsoides and Scenedesmus are cultured individually at a light intensity of 2500-4000 lux for 5-10 days at a temperature of 25-28℃, and then the single cultured microalgae are compounded to obtain a compound microalgae agent. The technical solution provided in this application can effectively degrade various pollutants in livestock and poultry wastewater, with significant treatment effects.

The invention relates to the technical field of probiotics, in particular to application of enterococcus faecium NSK220 in preparation of a product with a kidney protection function, the enterococcus faecium NSK220 is preserved in the China General Microbiological Culture Collection Center on January 6, 2025, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.33306. The invention further discloses a preparation method of the enterococcus faecium NSK220. The strain has anti-inflammatory, anti-oxidation and immunoregulation functions, and can be used for preparing products with kidney protection functions.

The invention discloses a humic acid type straw source microbial agent which comprises the following strains: enterococcus faecium, bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae. The method has the advantages that turfy soil, biogas residues, biogas slurry and a certain amount of agricultural waste are added into compost with straw microbial agents and livestock and poultry manure as main materials to adjust a proper carbon nitrogen ratio, and more importantly, a compound microbial agent for strengthening the process of the humic acid type straw source microbial agent is inoculated; compared with natural composting fermentation, the composting efficiency and stability are improved, and the fertilizer efficiency and fertility of the organic fertilizer and the resource utilization efficiency of waste are improved.

The application relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the application comprises the following components in parts by weight: saw leaf palm extract 1-8 parts, white birch mushroom extract 1-5 parts, Rhizoma Anemarrhenae extract 0.5-2.5 parts, raspberry extract 20-30 parts, sea-tortoise skin extract 3-5 parts, compound bacteria 3-5 parts and carrier 10-30 parts; further, the compound bacteria are selected from Enterococcus faecium, Bacillus laterosporus, Acetobacter orleanensis, Lactobacillus acidophilus and Lactobacillus rhamnosus; and the application also provides animal feed prepared from the compound feed additive. The compound feed additive provided by the application can improve the growth performance of animals from aspects of enhancing the immunity of animals, improving the intestinal microorganism balance of animals, improving the feed conversion rate and the like, so as to improve the breeding benefit of livestock and poultry breeding.

The invention discloses a material with probiotics coated with a tea polyphenol-based complex, a preparation method of the material and application of the material in relieving intestinal injury induced by nano-plastics, and relates to the technical field of material science. The material for coating the probiotics with the tea polyphenol-based complex comprises a core material and a shell material at least partially coating the surface of the core material, the core material comprises enterococcus faecalis, the enterococcus faecalis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the enterococcus faecalis is CGMCC 1.10682; the shell material comprises a complex of tea polyphenol and ferric ions. The material of the tea polyphenol-based complex coated probiotics is not limited by specific nano plastic types, has the advantages of being green, safe, edible and universal, and can effectively prevent the nano plastic from damaging intestinal barriers by promoting in-situ aging and agglomeration of the nano plastic in intestinal tracts, so that the nano plastic is discharged out of the body along with excrement, and intestinal tract protection is achieved.