A mir-318 that regulates insect wing development and its application in pest control

A technology of mir-318 and insects, applied in the fields of application, insecticide, plant growth regulator, etc., can solve the problem that miR-318 regulates the development of insect wings, which has not been reported, and achieves significant economic and social benefits, reducing The effect of winglet area

Active Publication Date: 2021-08-13
SHANDONG AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0006] miR-318 is one of many miRNAs, but there are few studies on the function of miR-318, and there is no report on the regulation of miR-318 on insect wing development

Method used

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  • A mir-318 that regulates insect wing development and its application in pest control
  • A mir-318 that regulates insect wing development and its application in pest control
  • A mir-318 that regulates insect wing development and its application in pest control

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Experimental program
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Effect test

Embodiment 1

[0035] 1. Overexpression of miR-318 significantly reduces the wing area of ​​Drosophila

[0036] UAS-GFP and UAS-miR-318 male flies were crossed with En-gal4 virgin flies specifically expressed in the wing posterior (P) region of Drosophila, and the adult wing phenotypes of the offspring were observed. The wings were photographed with a stereo microscope, and the areas of the P area and anterior (A) area were measured by ImageJ software, and the P / A ratio was calculated, compared with the control group, and statistically analyzed. Due to the difference in the individual size of offspring male Drosophila and female Drosophila, this study carried out statistics on the female and male individuals separately. The results of this study only show the wings and statistical results of male Drosophila adults.

[0037] The result is as figure 1 As shown, the P / A value of the control group was 1.3, and the P / A value decreased to 0.8 after miR-318 was overexpressed. Further statistical ...

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Abstract

The invention discloses a miRNA regulating insect wing development and its application in pest control. The present invention found that overexpression of miR-318 can significantly reduce wing area and inhibit wing development; further studies have found that its mechanism of action is: miR-318 directly inhibits the expression of Hippo signaling pathway transcription factor Scalloped (sd), and then Downstream of the inhibitory pathway promotes cell proliferation and inhibits the expression of apoptotic target genes, ultimately inhibiting wing development. The miR-318 of the present invention can significantly inhibit the development of insect wings, and provide important target gene resources for RNAi-mediated pest biological control by artificially synthesizing miR-318 or its analogs, or using transgenic plants expressing miR-318. It will provide important theoretical and practical significance for the control of Pteroptera pests, and also have significant economic and social benefits.

Description

technical field [0001] The invention relates to the fields of agricultural biotechnology and pest control, in particular to a miR-318 regulating insect wing development and its application in pest control. Background technique [0002] As an important organ of insects, wings endow insects with the ability to fly and increase the space for insects to feed and mate. At present, winged insects have become the focus and difficulty in the control of agricultural pests. Analyzing the molecular mechanism of insect wing development regulation and finding ways to inhibit wing development will provide important theoretical and practical significance for the control of winged insects. [0003] MicroRNA (miRNA) is a kind of small molecule non-coding RNA with a length of about 22nt, which is highly conserved in animal evolution. By recognizing the binding site on the 3'-UTR of the target gene mRNA, miRNA causes the transcriptional repression of the target gene or the degradation of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N57/16A01P7/04A01K67/033
CPCA01K67/0339A01K2227/706A01N57/16
Inventor 周紫章刘庆信赵云贺于璇
Owner SHANDONG AGRICULTURAL UNIVERSITY
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