Double-targeting magnetic fluorescent nano-microsphere, preparation method thereof and application of double-targeting magnetic fluorescent nano-microsphere in liver cancer circulating tumor cells

Abstract

The invention discloses a double-targeting magnetic fluorescent nano-microsphere, a preparation method thereof and an application of the double-targeting magnetic fluorescent nano-microsphere in livercancer circulating tumor cells. According to the nano-microsphere, a carboxyl-modified magnetic ferroferric oxide microsphere is used as a matrix, and anti-epithelial cell adhesion molecules and a fluorescent probe with strong affinity with aminopeptidase are introduced into the surface of magnetic ferroferric oxide at the same time, so that the double-targeting magnetic fluorescent nano-microsphere is obtained. On the basis of no damage of the cell activity (more than 90%), the double-targeting nano-microsphere greatly enhances the capture efficiency (more than 90%) and capture purity (morethan 90%) of the liver cancer circulating tumor cells. More importantly, the nanoparticle has accurate dual-targeting performance, high-resolution fluorescence imaging and excellent selectivity, so that the nano platform becomes a first case to realize real-time recognition imaging and monitoring of in-vivo circulating tumor cells.

Description

technical field [0001] The invention belongs to the technical field of biomaterial sensing, in particular to a dual-target magnetic fluorescent nanometer microsphere, its preparation method and its application in liver cancer circulating tumor cells. Background technique [0002] Liver cancer is a malignant and heterogeneous disease that causes nearly 1 million liver cancer-related deaths each year. High recurrence rate (almost 70% within 5 years after hepatectomy) and distant metastasis are the leading causes of HCC becoming the second leading cancer-related death. Traditional imaging diagnosis and staging assessment of circulating tumor cells in liver cancer have detection limitations, leading to an underestimation of the true extent of the disease. Therefore, in the complex biological research of liver cancer, it is urgent to find effective and representative biomarkers for early diagnosis, recurrence prediction and prognosis evaluation of liver cancer. Metastatic sprea...

AI Technical Summary

Problems solved by technology

However, considering the high recurrence rate and minimal number of HCC-CTCs in the bloodstream, using EpCAM as a single target to isolate CTCs, the detection rate is only between 30% and 80%.

The separation technology based solely on EpCAM mainly has the following disadvantages: (1) The experimental results of simulated CTCs in vitro are inconsistent with the experimental results in vivo, or due to the high heterogeneity and phenotypic changes of CTCs, resulting in missed capture and false negative signals; ( 2) False positive results due to release of benign epithelial cells into circulation due to inflammation or other liver disease

The disadvantages of these studies include: (1) long preprocessing time and low detection efficiency; (2) the morphology of CTCs changes during the extraction process; (3) it is difficult to achieve long-term real-time dynamic monitoring

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