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Method for relieving freshwater fish transportation stress

A technology for transportation stress and freshwater fish, which is applied in the field of alleviating freshwater fish transportation stress. It can solve the problems of single effect and poor quality of substances, and achieve the effects of ensuring muscle quality, reducing loss, and alleviating stress response

Active Publication Date: 2021-02-26
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the measures to alleviate the stress response during fish transportation are generally to add antioxidants or energy substances, such as vitamin C, glutathione, etc., but the effect is often not good due to the singleness and limitation of substances

Method used

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  • Method for relieving freshwater fish transportation stress
  • Method for relieving freshwater fish transportation stress
  • Method for relieving freshwater fish transportation stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 As shown, a method for alleviating freshwater fish transportation stress comprises the following steps:

[0042] 1) Configure the fermentation substrate: the pretreated astragalus, ginseng, angelica, medlar, and Ophiopogon japonicus, according to the mass ratio of astragalus, ginseng, angelica, medlar, and Ophiopogon japonicus respectively 10g:8g:18g:12g:15g Mix configuration to obtain fermentation substrate;

[0043] 2) Configuration of culture medium (nutrient solution):

[0044] Described step 2) Lactobacillus acidophilus agar medium: pancreas 10g, yeast extract powder 5g, potassium dihydrogen phosphate 6g, ammonium citrate 2g, sodium acetate 25g, magnesium sulfate 0.575g, manganese sulfate 0.15g, sub Ferric sulfate 0.0355g, glucose 20g, agar 15g, pH value 5.5±0.2, temperature 25°C, distilled water 1000ml;

[0045] Lactobacillus plantarum culture medium: yeast extract 7g, peptone 7g, glucose 10g, potassium dihydrogen phosphate 2g, tomato juice 100...

Embodiment 2

[0069] According to the process described in the specific embodiment, the fermentation broth was prepared and stored at low temperature for future use.

[0070] From 100 adult grass carp with a weight of 1.5±0.25kg purchased from the grass carp breeding base in Wuhan City, Hubei Province, 20 individuals in good health were selected as the transport group added with 50ml fermentation broth. The transport group was transported for 6 hours and then added an appropriate amount of MS- 222 was anesthetized and dissected in time to take the back muscles, and the indicators included in the muscles that could be used to measure the oxidative stress in the body included malondialdehyde (MDA), protein carbonyl, superoxide dismutase (SOD), catalase (CAT ), peroxidase (POD), glutathione peroxidase (GPX), glutathione (GSH), etc., tenderness, pH, pressurized water loss rate, muscle glycogen, Lactic acid and basic component content, and survival rate, etc.

[0071] Comparative example 1

[...

Embodiment 3

[0083] A method for alleviating freshwater fish transportation stress, comprising the steps of:

[0084] 1) Configure the fermentation substrate: the pretreated astragalus, ginseng, angelica, wolfberry, and Ophiopogon japonicus, according to the quality of 8g:6g:15g:10g:12g of astragalus, ginseng, angelica, medlar, and Ophiopogon japonicus Compared with the mixed configuration, the fermentation substrate is obtained;

[0085] 2) Configuration of culture medium (nutrient solution):

[0086] Lactobacillus acidophilus agar culture medium: 5g of pancreas cheese, 3g of yeast extract powder, 4g of potassium dihydrogen phosphate, 1g of ammonium citrate, 20g of sodium acetate, 0.5g of magnesium sulfate, 0.1g of manganese sulfate, 0.03g of ferric sulfite, and 10g of glucose , agar 10g, pH value 5.5±0.2, temperature 20°C, distilled water 1000ml;

[0087] Lactobacillus plantarum culture medium: yeast extract 6g, peptone 6g, glucose 8g, potassium dihydrogen phosphate 1g, tomato juice 80...

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Abstract

The invention belongs to the technical field of storage and transportation of aquatic products. The invention discloses a method for relieving freshwater fish transportation stress. The method is characterized by comprising the following steps of, 1) preparing a fermentation substrate, specifically, mixing astragalus membranaceus, ginseng, angelica sinensis, wolfberry fruits and radix ophiopogonisto obtain the fermentation substrate; 2) preparing a culture solution; 3) preparing inoculation liquid, specifically, selecting microorganisms according to a volume ratio of lactobacillus acidophilusto lactobacillus plantarum to bifidobacterium to streptococcus thermophilus of (1-4): (1-3): (1-6): (1-8); 4) treating the fermentation substrate obtained in the step 1); 5) fermenting; 6) adjustingthe pH value of extracted fermentation liquor to 7.0 by adopting sodium carbonate after the fermentation is finished, and filtering to obtain filtrate, specifically, the final fermentation liquor; and(7) adding the fermentation liquor obtained in the step (6) into a transportation water body of the freshwater fish. The method is natural and harmless, the survival rate of freshwater fish in the transportation process is increased, the loss of muscle nutrient components of fish body is reduced, oxidative damage of the fish body is relieved, and muscle quality is guaranteed to a great extent.

Description

technical field [0001] The invention belongs to the technical field of aquatic product storage and transportation, and in particular relates to a method for alleviating the transportation stress of freshwater fish. Background technique [0002] Freshwater fish resources are an important part of China's water resources, and its annual output and aquaculture area rank first in the world. With the development of society and the continuous improvement of human living standards, people's demand for food is no longer limited to being full, but more importantly, nutrition and health. They have a higher pursuit of food quality and safety. Compared with other animal foods, freshwater fish is one of the main sources of protein in the national food. The meat is tender, unique in taste and rich in nutrition. The cholesterol content is almost zero, and it is extremely rich in high unsaturated fatty acids and vitamin D, which are beneficial to human health. Substance, suitable for consum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K50/80A23K10/12A23K10/30
CPCA01K61/10A23K50/80A23K10/12A23K10/30A23V2400/113A23V2400/169A23V2400/51A23V2400/249Y02A40/81Y02A40/818
Inventor 汪兰石柳王俊熊光权吴文锦尤孝鹏丁安子乔宇李新廖李
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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