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Biomarker composition for detecting gastric cancer autoantibody in gastritis patient and application

A technology for biomarkers and patients with gastritis, applied in biological testing, measuring devices, material inspection products, etc., can solve the problem of not finding markers for detecting gastric cancer autoantibodies, etc., and achieve the effect of improving sensitivity and accuracy and good diagnosis

Active Publication Date: 2021-02-26
RAYBIOTECH INC GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cancer autoantibodies are the specific responses of the immune system against cancer cells, and have the characteristics of high sensitivity and high specificity. However, no markers have been found that can detect gastric cancer autoantibodies from patients with gastritis.

Method used

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  • Biomarker composition for detecting gastric cancer autoantibody in gastritis patient and application
  • Biomarker composition for detecting gastric cancer autoantibody in gastritis patient and application
  • Biomarker composition for detecting gastric cancer autoantibody in gastritis patient and application

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The embodiment of the present application provides a screening test for detecting a combination of biomarkers for gastric cancer autoantibodies from patients with gastritis, and the specific steps are as follows:

[0043] 1. Construction of a detection protein chip for detecting gastric cancer autoantibodies from patients with gastritis

[0044] 1. In this example, genetic engineering is used to produce and identify various human proteins using prokaryotic and eukaryotic expression platforms. The target gene is amplified by RT-PCR and cloned into a bacterial expression vector. After the correctness of the gene cloning vector is verified by DNA sequencing, the recombinant material is transformed into competent expression bacteria. After gene induction and expression, a variety of proteins were obtained by affinity chromatography and high performance liquid chromatography purification from the lysate or inclusion body.

[0045] 3. Immobilize a variety of proteins as anti...

Embodiment 2

[0050] The embodiment of the present application provides a confirmatory test for detecting gastric cancer autoantibody biomarker combination ADAM17, MIP-1β and MMP-7 from gastritis patients, and the specific steps are as follows:

[0051] 1. The test samples were selected from patients with chronic gastritis (77 cases in total) and patients with diffuse gastric cancer (47 cases in total). The serum of the above samples was extracted to obtain the IgA and IgG of the serum of the above 124 samples.

[0052] 2. The IgA and IgG of the sera of 124 samples in step 1 were immunoreacted with the protein chip for gastric cancer diagnosis of Example 1, respectively, and then the anti-IgG antibody and anti-IgA antibody coupled with detectable biotin were respectively Antibodies corresponding to the above-mentioned chips were incubated, and the responses of IgA and IgG in the serum of 124 samples to the biomarkers (hereinafter referred to as targets) of the protein chip for gastric cancer...

Embodiment 3

[0058] The embodiment of the present application provides a confirmatory test for detecting gastric cancer autoantibody biomarker combination ADAM17, MIP-1β and MMP-7 from gastritis patients, and the specific steps are as follows:

[0059] 1. The test samples were selected from patients with chronic gastritis (77 cases in total) and patients with intestinal type gastric cancer (77 cases in total). The serum of the above samples was extracted to obtain the IgA and IgG of the serum of the above 154 samples.

[0060] 2. The IgA and IgG of the sera of 154 samples in step 1 were immunoreacted with the protein chip for gastric cancer diagnosis of Example 1, respectively, and then the anti-IgG antibody and anti-IgA antibody coupled with detectable biotin were respectively Antibodies corresponding to the above-mentioned chips were incubated, and the responses of IgA and IgG in the serum of 154 samples to the biomarkers (hereinafter referred to as targets) of the protein chip for gastri...

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Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to a biomarker composition for detecting a gastric cancer autoantibody in a gastritis patient and application of the biomarker composition, which can be used for detecting the autoantibody in serum of the gastric cancer patient in the gastritis patient. The invention provides a biomarker combination for detecting a gastric cancer autoantibody in a gastritis patient. The biomarker combination comprises depolymerized metalloproteinase 17, macrophage inflammatory protein-1beta and matrix metalloproteinase-7. The invention provides a biomarker composition for detecting a gastric cancer autoantibody in a gastritis patient. The biomarker composition can effectively overcome the technical defects that an existing marker is not high in specificity, sensitivity and accuracy when used for detecting the gastric cancer autoantibody.

Description

technical field [0001] The application belongs to the technical field of molecular biology, and in particular relates to a biomarker combination and application for detecting gastric cancer autoantibodies in patients with gastritis. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world, and it is also one of the malignant tumors with the highest mortality rate. Most gastric cancer patients have missed the best opportunity for diagnosis and treatment when they are diagnosed, leading to disease progression, tumor metastasis, and even entering the terminal stage. From the perspective of TMN staging of gastric cancer, the 5-year survival rate of gastric cancer is 97.6% in the early stage of the first stage, 94.9% in the late stage of the first stage, 70.49% in the second stage, 56.7% in the early stage of the third stage, and 56.7% in the late stage of the third stage. It was 31.9%, and the fourth period was 6.5%. Visible early ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/68G01N33/573
CPCG01N33/57488G01N33/57446G01N33/6854G01N33/573
Inventor 黄若磐罗树红符聪聪琼·克拉布特里
Owner RAYBIOTECH INC GUANGZHOU
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