The invention also discloses application of plant polysaccharide as adjuvant in preparation of medicines for treating autoimmune diseases
An immune disease, plant-based technology, applied in allergic diseases, metabolic diseases, bone diseases, etc., can solve the problem of lack of adjuvants, and achieve the effect of inducing immune tolerance, delaying the onset, and reducing the titer.
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Embodiment 1
[0031] 1. Vaccines for autoimmune diseases
[0032] The above autoimmune disease vaccine includes inulin and insulin antigen, the mass concentration of inulin in the vaccine is 2%, and the particle size of inulin is 250nm;
[0033] The mass concentration of insulin antigen in the vaccine is 2%.
[0034] 2. Preparation method
[0035] The preparation method of the vaccine of above-mentioned autoimmune disease, this preparation method comprises the steps:
[0036] (a) Dissolving 100mg inulin and 100mg insulin antigen in 10ml dimethyl sulfoxide to obtain a mixed solution;
[0037] (b) placing the mixed solution in a dialysis bag with a molecular weight cut-off of 5000, and using 2000ml of sterile water to dialyze at room temperature for 40 hours, changing the solution every hour during the dialysis process to obtain nanoparticles;
[0038] (c) placing the nanoparticles in PBS buffer solution to prepare the corresponding concentration to obtain the above autoimmune disease vacc...
Embodiment 2
[0040] 1. Vaccines for autoimmune diseases
[0041] The above autoimmune disease vaccine includes glucomannan and insulin antigen, the mass concentration of glucomannan in the vaccine is 1%, and the particle size of glucomannan is 200-700nm;
[0042] The mass concentration of insulin antigen in the vaccine is 4%.
[0043] 2. Preparation method
[0044] The preparation method of the vaccine of above-mentioned autoimmune disease, this preparation method comprises the steps:
[0045] (a) Dissolving 20 mg of glucomannan and 80 mg of insulin antigen in 10 ml of dimethyl sulfoxide to obtain a mixed solution;
[0046] (b) placing the mixed solution in a dialysis bag with a molecular weight cut-off of 5000, and using 2000ml of sterile water to dialyze at room temperature for 40 hours, changing the solution every hour during the dialysis process to obtain nanoparticles;
[0047] (c) placing the nanoparticles in PBS buffer solution to prepare the corresponding concentration to obtain...
Embodiment 3
[0049] 1. Vaccines for autoimmune diseases
[0050] The above autoimmune disease vaccine includes β-1,3-D-glucan and insulin antigen, the mass concentration of β-1,3-D-glucan in the vaccine is 2.5%, β-1,3- The particle size of D-glucan is 200-700nm;
[0051] The mass concentration of the insulin antigen in the vaccine is 1%.
[0052] 2. Preparation method
[0053] The preparation method of the vaccine of above-mentioned autoimmune disease, this preparation method comprises the steps:
[0054] (a) Dissolving 50 mg of β-1,3-D-glucan and 20 mg of insulin antigen in 5 ml of dimethyl sulfoxide to obtain a mixed solution;
[0055] (b) placing the mixed solution in a dialysis bag with a molecular weight cut-off of 3000, and using 1000ml of sterile water to dialyze at room temperature for 30 hours, and changing the solution every hour during the dialysis process to obtain nanoparticles;
[0056] (c) placing the nanoparticles in PBS buffer solution to prepare the corresponding concen...
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Abstract
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