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Method for determining seven degradation products of indoxacarb in tea leaves and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry

An ultra-high performance liquid phase and tandem mass spectrometry technology, which is applied to the field of 7 degradation products of indoxacarb, can solve the problems of seldom involving degradation products and only focusing on the parent body of indoxacarb

Active Publication Date: 2021-03-02
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods and studies only focus on the parent body of indoxacarb, and seldom involve the research on its degradation products

Method used

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  • Method for determining seven degradation products of indoxacarb in tea leaves and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry
  • Method for determining seven degradation products of indoxacarb in tea leaves and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry
  • Method for determining seven degradation products of indoxacarb in tea leaves and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry

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Experimental program
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Embodiment

[0024] 1. Experimental part

[0025] 1.1 Main instruments and equipment

[0026] UPLC / Quattra Premier XE Ultra High Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry Instrument: Product of Waters, USA, equipped with electrospray ionization (ESI) source, MassLynx 4.1 mass spectrometry workstation; chiral chromatographic column: 3 μm Cellulose-1, 3μm Cellulose-2, 3μm×150mm×2mm, product of American Phenomenex Company; Strata-X 33u PRP (polymeric reversed phase) SPE column: 500mg / 6mL, product of American Phenomenex Company; BondElut C18 SPE column, 500mg / 6mL, American Agilent Company Products; 3K-5 refrigerated high-speed centrifuge: product of Sigma, Germany; R-210 rotary evaporator: product of BUCHI Labortechnik A G, Switzerland; KQ-250DB CNC ultrasonic cleaner: product of Kunshan Ultrasonic Instrument Co., Ltd.; Vortex Genie2 vortex Rotary vibrator: product of American Scientific Company; T-18 high-speed homogenizer: product of German IKA company; DFT-20...

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Abstract

The invention discloses a method for determining seven degradation products of indoxacarb in tea leaves and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry, andbelongs to the technical field of tea pesticide residue detection. The method comprises the following steps: 1) sample extraction and purification: extracting a fresh leaf or tea sample with acetonitrile / water, carrying out C18 and GCB dispersive solid-phase extraction and purification, concentrating, and fixing the volume with methanol / water; or 1) enriching, purifying and leaching the PRPSPE column of the tea soup sample, eluting and receiving with methanol, concentrating, and fixing the volume with methanol / water; 2) performing ultra-high performance liquid chromatography tandem mass spectrometry by adopting C-2 chromatographic column separation to detect seven degradation products of indoxacarb; and (3) calculating the standard curve and the linear correlation coefficient of the indoxacarb degradation products, and (4) calculating the adding recovery rate, the relative standard deviation, the detection limit and the quantification limit. The method disclosed by the invention meetsresidue analysis requirements and can provide an analysis method for research and detection of seven degradation products of indoxacarb in fresh tea leaves, tea leaves and tea soup.

Description

technical field [0001] The invention belongs to the technical field of detection of pesticide residues in tea, and in particular relates to a method for determining seven degradation products of indoxacarb in tea and tea soup based on ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] my country is the origin of tea, and it is also the world's largest producer and consumer of tea. Tea is of great significance to the economic development of the vast tea areas and the poverty alleviation of tea farmers in mountainous areas. Tea is a green and healthy beverage, and its quality and quality safety are related to the long-term sustainable development of the tea industry. Tea grows in a hot and humid environment, and pests and diseases are relatively easy to occur. Spraying chemical pesticides is the fastest and most effective way to prevent and control tea tree pests and diseases. Indoxacarb has a unique and novel structure and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/16G01N30/34G01N30/60G01N30/72
CPCG01N30/02G01N30/06G01N30/16G01N30/34G01N30/60G01N30/72G01N2030/062G01N2030/047
Inventor 张新忠钟青陈宗懋罗逢健黎洪霞
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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