Sample adding needle for preparing micro-droplets and preparation method of micro-droplets
A technology for adding sample needles and microdroplets, which is applied in chemical instruments and methods, fluid controllers, measuring tubes/pipettes, etc. Uniform, volume-controllable effect
Active Publication Date: 2021-03-05
MACCURA MEDICAL INSTR CO LTD
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- Description
- Claims
- Application Information
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Problems solved by technology
The second is to use a special device to spray a small amount of liquid to form micro-droplets, such as special injection or micro-droplet excitation methods such as piezoelectric ceramics, thermal expansion, high-voltage electrospray, etc. Regulation is difficult, and biological samples may be damaged to a certain extent
Method used
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Embodiment 1
[0103] The liquid storage volume of the sample needle 110 described in the present application is 60 microliters, and the material for preparing the sample needle 110 is polypropylene (PP, the contact angle of pure aqueous solution is 88 degrees), the height of the spit part 120 is 5 mm, and the taper is 20 degrees. °, the wall thickness is 0.15mm, the height of the liquid storage part 130 is 18.7mm, the taper is 4°, the wall thickness is 0.5mm, the height of the adapter part is 6mm, the taper is 4°, the inner diameter of the spit opening 150 is 100μm, and the outer The diameter is 400 μm. According to the above dimensions, the precision metal injection mold cavity and inner core are made, and the injection molding process is used for large-scale automatic sample needle processing. The processing yield is 99.98% (batch testing quantity 10,000 pieces).
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Abstract
The invention discloses a sample adding needle for preparing micro-droplets. The sample adding needle comprises a liquid storage part and a liquid discharge part which are integrally formed in an injection molding mode and are communicated with each other, the liquid storage part is a circular truncated cone with the radial size gradually decreased in the direction towards the liquid discharge part, and the liquid discharge part is a circular truncated cone with the radial size gradually decreased in the direction away from the liquid storage part. The taper of the liquid storage part is C1, the taper of the liquid discharge part is C2, and C1 is smaller than or equal to C2. The wall thickness of the liquid storage part is D1, the wall thickness of the liquid discharge part is D2, and D1 is greater than D2. According to the sample adding needle for preparing micro-droplets, when the micro-droplets are prepared through the sample adding needle, the sample adding needle does periodic reciprocating motion with changed speed in oily liquid, so that the sample solution is subjected to periodic shearing force of the oily liquid at a liquid discharge opening, further, the sample solutionin the sample adding needle enters the oily liquid, and the generation of micro-droplets with uniform size and controllable volume is realized.
Description
technical field [0001] The application relates to the fields of microfluidic technology, micro-scale material preparation, micro-reactor and micro-analysis technology, and specifically relates to a sampling needle for preparing micro-droplets and a method for preparing micro-droplets. Background technique [0002] Microdroplets are widely used in various fields. Microfluidic technology based on microdroplets has achieved rapid progress in the fields of single cell analysis, single cell sequencing, digital PCR, protein crystallization, high-throughput reaction screening, and single cell functional sorting. development and application. [0003] The generation of micro-droplets is to generate emulsified micro-droplets by using two immiscible phases. The micro-droplet phase is called the dispersed phase, and the phase that wraps the micro-droplets is called the continuous phase. After the micro-droplets are generated, they can be divided, fused, mixed, diluted, collected and so...
Claims
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IPC IPC(8): B01L3/02B01L3/00
CPCB01L3/0251B01L3/502784B01L3/0241B01L3/0275B01L2200/0673B01L2400/0433B01L2200/027B01L2300/0838B01L2400/0406
Inventor 翁蓉蓉
Owner MACCURA MEDICAL INSTR CO LTD
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