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Metschnikowia bicuspidate and method for treating high-salinity wastewater

A high-salt wastewater and yeast technology, applied in the direction of microbial-based methods, chemical instruments and methods, and food industry wastewater treatment, can solve problems such as high cost and affect the production efficiency of processing plants, and achieve low cost and simple operation Effect

Pending Publication Date: 2021-03-16
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the high-salt wastewater produced by kelp and wakame processing can be treated by using the MVR high-efficiency evaporation and crystallization system, the cost is relatively high (100-120 yuan / ton), which directly affects the production efficiency of the processing plant

Method used

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  • Metschnikowia bicuspidate and method for treating high-salinity wastewater
  • Metschnikowia bicuspidate and method for treating high-salinity wastewater
  • Metschnikowia bicuspidate and method for treating high-salinity wastewater

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Effect test

Embodiment 1

[0024] In the present invention, after separation, purification, identification, optimization and screening, the strain that can utilize the organic matter of high-salt wastewater is M. trichomes ( Metschnikowia bicuspidate ) ， It has been deposited in the General Microbiology Center of the China Microorganism Culture Collection Management Committee, codenamed WSKeW-M, and the deposit number is CGMCC No.18674.

[0025] The method for processing high-salt wastewater with the above-mentioned M. biloba is carried out according to the following steps:

[0026] a. Gradually expand the seed solution of the above-mentioned M. biloba (CGMCC No.18674);

[0027] b. Add 8 times the volume of the expanded bacterial solution to the high-salt wastewater produced by kelp processing and the high-salt wastewater produced by wakame processing at a volume percentage of 5%, and shake at 120 r / min at 20 °C. Incubate for 60 hours.

[0028] Measure the high-salt waste water produced by kelp proc...

Embodiment 2

[0030] The method for processing high-salt wastewater with the above-mentioned M. biloba is carried out according to the following steps:

[0031] a. Combine the above-mentioned M. biloba (CGMCC No. 18674) with the Pichia moniliformes with the preservation number of CGMCC No. 18675 ( Meyerozyma guilliermondii ) according to the volume ratio of 1:1 to make compound seed solution and gradually expand the culture;

[0032] b. Add 8 times the volume of the expanded bacterial solution to the high-salt wastewater produced by kelp processing at a volume percentage of 3%, and culture at 20 °C with a shaker at 150 r / min for 60 hours.

[0033] The high-salt waste water produced by the processing of kelp processed by the above-mentioned method is measured, and the results are as follows: image 3 shown. The results showed that the COD removal rate of high-salt wastewater produced by kelp processing was 85.98%.

Embodiment 3

[0035] The method for processing high-salt wastewater with the above-mentioned M. biloba is carried out according to the following steps:

[0036] a. Combine the above-mentioned M. biloba (CGMCC No. 18674) with Halomonas with the preservation number of CGMCC No. 20812 ( Cobetia sp. ) according to the volume ratio of 2:1 to make compound seed solution and gradually expand the culture;

[0037] b. Add the 20-fold volume of the bacterial solution to the high-salt wastewater from wakame processing at a volume percentage of 1%, and culture at 20 °C with a shaker at 150 r / min for 60 hours.

[0038] Using potassium permanganate oxidation method to carry out COD determination on the high-salt wastewater produced by wakame processing that has been processed by the above method, the results are as follows: Figure 4 shown. The results showed that the COD removal rate of high-salt wastewater from wakame processing was 86.24%.

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Abstract

The invention discloses Metschnikowia bicuspidate which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has a preservation number being CGMCC No.18674. The invention discloses a method for treating high-salinity wastewater by using Metschnikowia bicuspidate. The method comprises the following steps: gradually carrying out enlarged culture on a metschnikowiabicuspidate seed solution; and adding the bacterial liquid with the volume multiplied by the enlarged culture into the high-salinity wastewater, and performing cultivation. The method is simple to operate and low in cost, and has a COD removal rate on kelp processing produced high-salinity wastewater being 82.92% and a COD removal rate on undaria pinnatifida processing produced high-salinity wastewater being 85.60% to reach the emission standard of city pipe network.

Description

technical field [0001] The present invention relates to a kind of bacterium and treatment method for waste water treatment, especially a kind of M. biloba (Latin name) Metschnikowia bicuspidate, Preservation date: 2019-10-12; Preservation unit: General Microbiology Center of China Microbial Culture Collection Management Committee; Preservation Number: CGMCC No.18674) and method for treating high-salt wastewater. Background technique [0002] In order to prevent the spoilage of fresh kelp and wakame, the harvested fresh vegetables are usually blanched, cooled and salted immediately to achieve sterilization and dehydration. The blanching water is seawater that is repeatedly heated to about 95 degrees. After 8~10 hours of recycling, it is high-salt wastewater. The content of organic matter (algin, mannitol, etc.) and inorganic matter is high, and the COD value can reach 1200~ 2100, if this high-salt wastewater is directly discharged, it will cause seawater pollution, so it nee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/645C12R1/84C02F101/30C02F103/32
CPCC12N1/16C02F3/347C02F3/348C02F2101/30C02F2103/32
Inventor 汪秋宽何云海杨婷婷刘舒任丹丹武龙周慧宋悦凡
Owner DALIAN OCEAN UNIV
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