Composition and kit for methylation noninvasive early detection of ovarian cancer

An early detection and methylation technology, applied in the field of nucleic acid in vitro diagnosis, can solve the problem of less circulating DNA in tumors

Active Publication Date: 2021-03-16
BEIJING ORIGIN-POLY BIO-TEC CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amount of tumor circulating DNA in plasma is small and prone to degradation

Method used

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  • Composition and kit for methylation noninvasive early detection of ovarian cancer
  • Composition and kit for methylation noninvasive early detection of ovarian cancer
  • Composition and kit for methylation noninvasive early detection of ovarian cancer

Examples

Experimental program
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Embodiment 1

[0071]Detection test for detecting ovarian cancer-related genes CDH13, FOXD3, ZNF671, HOXA11, FBXO32 and OPCML methylation kit.

[0072] The kit components are as follows:

[0073]

[0074] Select 86 cases of ovarian cancer samples with known clear pathological information: 50 cases were identified as high-grade serous carcinoma, 13 cases of low-grade serous carcinoma, 12 cases of clear cell carcinoma, 11 cases of mucinous carcinoma; 50 cases were identified as benign ovarian tumors sample.

[0075] 1. Using Tiangen Biochemical Technology (Beijing) Co., Ltd.’s magnetic bead method large-volume free nucleic acid extraction kit (DP710) to extract plasma free DNA from the above-mentioned 136 cases of ovarian cancer and ovarian benign tumor samples, and at the same time perform DNA quality control .

[0076] 2. Using the DNA bisulfite conversion kit (DP215) of Tiangen Biochemical Technology (Beijing) Co., Ltd. to perform bisulfite conversion on the extracted DNA, the unmethyl...

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Abstract

The invention discloses a composition and kit for methylation noninvasive early detection of ovarian cancer. The composition comprises target gene detection primers and internal reference gene detection primers, wherein target genes are CDH13, FOXD3, ZNF671, HOXA11, FBXO32 and OPCML. The detection primers for the genes not only comprise promoter regions of the genes, but also comprise coding regions of the genes, and multi-gene methylation regions are selected in the composition for joint detection, so that functional complementation is formed, the sensitivity of detecting the ovarian cancer is remarkably improved, and the detection primers have very high specificity for normal and ovarian benign tumors. The kit containing the detection composition can detect patients with the possibilityof the ovarian cancer in advance by utilizing a methylation detection technology through a molecular epigenetic means, so that prevention and treatment can be carried out in advance.

Description

technical field [0001] The invention relates to the field of nucleic acid in vitro diagnosis, in particular to the application of specific gene methylation markers to the early detection of ovarian cancer and its auxiliary diagnosis. Background technique [0002] In my country, the incidence of ovarian cancer ranks third in gynecological malignancies, accounting for about 23% of all female reproductive tract tumors, showing an increasing trend year by year. About 25,000 women die of ovarian cancer every year in my country, ranking first among gynecological malignancies. Because the ovary is deep in the pelvic cavity, small in size, and lacks typical symptoms, it is difficult to detect early. When it is diagnosed, 60%-70% are in the advanced stage, and its 5-year survival rate is 20%-30%. Ovarian cancer patients are found to be confined to the ovary during surgery. Accounted for less than 30%, most have spread to the pelvic and abdominal organs. The 5-year survival rate of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/166C12Q2600/154C12Q2600/16C12Q2531/113C12Q2537/143C12Q2563/107C12Q2523/125C12Q2521/101
Inventor 刘沛王林海王紫芸
Owner BEIJING ORIGIN-POLY BIO-TEC CO LTD
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