Lactobacillus plantarum for skin care
A Lactobacillus plantarum, dermatological technology, applied in the field of microorganisms to improve skin appearance, treat and/or prevent skin problems
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[0146] Preparation of Heat-treated Lactobacillus Suspension
[0147] Growth of Lactobacillus plantarum strains HEAL 19 and HEAL 99 can be obtained in various media. The carbon source can be glucose or starch. Meat extract, yeast extract, peptone or peptone (vegetable) etc. can be used as nitrogen source. The pH of the medium should be around 6. The incubation temperature is variable, but 37°C is preferred. The incubation time may be about 8 to 24 hours. Shocks or inflatable shocks can be added.
[0148] After incubation, a washing process can be added. This can be done as follows. Cells can first be collected by centrifugation, the supernatant decanted, and the cell pellet suspended with liquid PBS, cell culture medium, or purified water. The above process can be repeated if necessary.
[0149] The heat treatment of the lactobacillus suspension can be performed before or after the washing process. This can be achieved by ultrasound, UV irradiation or heating, howeve...
example 1
[0201] Example 1: AMP gene expression in HaCa keratinocytes
[0202] Lactobacillus plantarum HEAL 19 induces the release of multiple antimicrobial peptides in human keratinocytes. All these peptides together represent the complex defense system of the human skin. For example, defensins, which kill Gram-positive bacteria, are upregulated. In conclusion, the upregulated peptides are effective against a wide range of potential pathogenic Gram-positive and Gram-negative bacteria, fungi, eukaryotic parasites and / or viruses.
[0203] figure 1 Gene expression of antimicrobial peptides in HaCaT keratinocytes after treatment with 0.05% L. plantarum HEAL 19 for 24 hours is shown. Gene expression has been measured using gene arrays; induction was defined as a calculated RQ value >2.
[0204]The results showed that treatment with L. plantarum HEAL 19 could induce important AMPs such as β-defensins 1, 3 or 4 and / or psoriasis and / or calprotectin. This treatment resulted in higher lev...
example 2
[0208] Example 2: Anti-inflammatory effects of Lactobacillus strains (in vitro / ex vivo)
[0209] Skin diseases, lesions or irritations are mostly associated with inflammation of the affected skin area. The results show that, in addition to the beneficial effects of the described Lactobacillus strains, their topical application also has an anti-inflammatory effect. In this regard, Lactobacillus strains support skin health through another mechanism.
[0210] Figure 4 The anti-inflammatory effect of L. plantarum HEAL 19 and 99 and dexamethasone, which was induced as a positive control, is shown. Fused HaCaT keratinocytes were pretreated with test materials 0.1 mg / mL Lactobacillus plantarum HEAL99, 0.25 mg / mL Lactobacillus plantarum HEAL 19 and 30 μM dexamethasone. After 48 hours of incubation, keratinocytes were stimulated with 30 ng / mL of pro-inflammatory IL-ld for 8 hours. The subsequent release of IL-8 in the cell culture medium was measured using ELISA. By administeri...
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