SNP molecular marker 1995 related to fusarium head blight resistance and application
A technology of wheat scab and molecular marker
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[0031] Screening of SNP Molecular Markers Related to Wheat Scab Resistance
[0032] 1. Materials and methods
[0033] 1.1 Plant material
[0034] For association analysis of 205 wheat materials, see Chen Guangfeng, Chen Jiansheng, Tian Jichun. Genome-wide association analysis of wheat plant height-related traits and SNP markers[J]. Acta Crops, 2015.
[0035] Select Sumai 3 as the high resistance control, Shannong 102 as the moderate resistance control, and Ningmai 22 as the moderate resistance control.
[0036] 1.2 Bacteria solution preparation
[0037] The present invention adopts 7136, F301, F609, F15 four kinds of wheat scab pathogenic bacteria with strong pathogenicity provided by Nanjing Agricultural University to prepare mixed conidia liquid; the pathogenic bacteria are inoculated in mung bean culture medium, 1500rpm shakes 5- After 7 days, filter after culturing, prepare conidia suspension, and observe the concentration of conidia under a microscope until the number ...
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