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Flow cytometry detection device, preparation method and system

A technology of flow cytometry and detection device, which is applied to measurement devices, instruments, particle and sedimentation analysis, etc., can solve the problems of difficulty in measuring the refractive index of single cell surface, change in refractive index, and difficult to extract, etc., and achieve high-throughput Single-cell detection, the effect of reducing the cost of detection and reducing the time-consuming of detection

Active Publication Date: 2021-03-19
INST OF MICROELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

However, due to the similar composition of the cell surface material in different states, the refractive index change is small, and the signal from the cell surface layer is often mixed with the internal signal of the cell, which is difficult to extract separately
Therefore, it is difficult to measure the refractive index of single cell surface

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  • Flow cytometry detection device, preparation method and system
  • Flow cytometry detection device, preparation method and system
  • Flow cytometry detection device, preparation method and system

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Embodiment Construction

[0027] In order to better understand the technical solutions provided by the embodiments of this specification, the technical solutions of the embodiments of this specification will be described in detail below through the drawings and specific examples. The detailed description of the technical solutions of the embodiments is not a limitation to the technical solutions of this specification. In the case of no conflict, the embodiments of this specification and the technical features in the embodiments can be combined with each other.

[0028] In the first aspect, the embodiment of this specification provides a flow cytometric detection device, such as figure 1 As shown, the flow cytometric detection device 10 includes: a surface plasmon excitation chip 110 and a cell channel 120 .

[0029] The surface plasmon excitation chip 110 includes a transparent substrate 101 and a metal film layer 102 attached to the surface of the substrate 101 for exciting surface plasmons under the ...

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Abstract

The invention discloses a flow cytometry detection device, a preparation method and a system. The device comprises a surface plasmon excitation chip which is used for exciting surface plasmon under the irradiation of polarized light and comprises a transparent substrate and a metal film layer attached to the surface of the substrate; and a cell channel which comprises an inflow channel, a compression channel and a recovery channel, and the cell channel is bonded with the surface, attached with the metal film layer, of the chip to form a waterproof closed space, wherein a metal film layer is attached to the coverage area of the compression channel. When the device is in a working state, to-be-detected cells carried by a solution flow in from the flow-in channel, enter the compression channel, pass through the target detection area in a state of keeping in contact with the metal film layer, and flow out from the recovery channel. The device can effectively realize rapid detection of thesurface refractive index of a single to-be-detected cell.

Description

technical field [0001] The invention relates to the technical field of photoelectric detection, in particular to a flow cytometry detection device, preparation method and system. Background technique [0002] As the basic unit of the structure and function of organisms, cell research is of great significance to reveal the chemical nature and laws in the life process. Due to the limitation of research methods, the past life science research mainly measured the comprehensive characteristics of a large number of cells. However, there are significant microscopic heterogeneities among different individuals of the same type of cells, and the experimental results based on the comprehensive characteristics of a large number of cells are difficult to reflect the single-cell level. Realizing the law of life activities of human beings, realizing single-cell analysis has become one of the development directions of measurement technology. At the same time, the microscopic heterogeneity ...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1434
Inventor 刘虹遥赵阳路鑫超王雪孙旭晴黄成军
Owner INST OF MICROELECTRONICS CHINESE ACAD OF SCI
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