A method for sterilizing experimental consumables by irradiation

A technology of irradiation sterilization and experiments, applied in the direction of liquid cleaning methods, irradiation, chemical instruments and methods, etc., can solve the problems of ineffective sterilization of high-temperature-resistant strains, inability to promote, and harmfulness to human body, etc., to achieve The sterilization effect is remarkable, the irradiation time is short, and the effect of ensuring cleanliness

Active Publication Date: 2022-04-01
广州华大生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ethylene oxide disinfection method has the defects of polluting the atmosphere and leaving harmful residues on medical devices, so it cannot be widely promoted
Patent CN209253678U discloses an autoclave for laboratory petri dishes, which can effectively sterilize laboratory petri dishes at high temperature; however, autoclaving methods usually need to be carried out in a high temperature and high pressure environment. When the high temperature and high pressure exceed a certain limit, The high temperature and high pressure environment will have a certain impact on the performance of polypropylene experimental consumables; when the high temperature and high pressure is lower than a certain limit, the sterilization effect on high temperature resistant bacteria is not obvious

Method used

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  • A method for sterilizing experimental consumables by irradiation
  • A method for sterilizing experimental consumables by irradiation
  • A method for sterilizing experimental consumables by irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for sterilizing a petri dish by irradiation, comprising the steps of:

[0028] S01: The petri dish is cleaned by ultrasonic for the first time, the ultrasonic power is 80W, the ultrasonic frequency is 30kHz, and the ultrasonic power density is 7.1W cm -2 , the ultrasonic time is 2 minutes; the cleaning solution used in the first ultrasonic cleaning includes the following components in parts by weight: porous nano-silicon 6kg, sodium lauryl sulfate 6kg, ethylene glycol n-butyl ether 5kg, phenol 4kg, 0.5kg of isopropanol and 78.5kg of water; wherein, the light stabilizer is added to the polypropylene by the blending method in the petri dish, and the weight ratio of the light stabilizer to polypropylene is 0.5:100; : 1.5 of 1-(methyl)-8-(1,2,2,6,6-pentamethyl-4-piperidine) sebacate and bis(3,5-tertiary butyl-4- Hydroxyphenyl) sulfide composition.

[0029] S02: The petri dish is cleaned by ultrasonic for the second time, the ultrasonic power is 80W, the ultrasoni...

Embodiment 2

[0034] A method for sterilizing a petri dish by irradiation, comprising the steps of:

[0035] S01: The petri dish is cleaned by ultrasonic for the first time, the ultrasonic power is 80W, the ultrasonic frequency is 30kHz, and the ultrasonic power density is 7.1W cm -2 , the ultrasonic time is 2 minutes; the cleaning solution used in the first ultrasonic cleaning includes the following components in parts by weight: porous nano-silicon 7kg, sodium lauryl sulfate 4kg, ethylene glycol n-butyl ether 7kg, phenol 2kg, 1kg of isopropanol and 79kg of water; wherein, the light stabilizer is added to the polypropylene by the blending method in the petri dish, and the weight ratio of the light stabilizer to polypropylene is 1:100; the weight ratio of the light stabilizer is 1:3.5 1-(methyl)-8-(1,2,2,6,6-pentamethyl-4-piperidine) sebacate and bis(3,5-tertiary butyl-4-hydroxybenzene base) sulfide composition.

[0036] S02: The petri dish is cleaned by ultrasonic for the second time, th...

Embodiment 3

[0041] A method for sterilizing a petri dish by irradiation, comprising the steps of:

[0042] S01: The petri dish is cleaned by ultrasonic for the first time, the ultrasonic power is 80W, the ultrasonic frequency is 30kHz, and the ultrasonic power density is 7.1W cm -2 , the ultrasonic time is 2 minutes; the cleaning solution used in the first ultrasonic cleaning includes the following components in parts by weight: porous nano-silicon 6.5kg, sodium lauryl sulfate 5kg, ethylene glycol n-butyl ether 6kg, phenol 3kg , Virahol 0.8kg and water 78.7kg; Wherein, culture dish adopts blending method to add light stabilizer in polypropylene, the weight ratio of light stabilizer and polypropylene is 0.8:100; Light stabilizer is by weight ratio: 1:2.5 of 1-(methyl)-8-(1,2,2,6,6-pentamethyl-4-piperidinium) sebacate and bis(3,5-tertiary butyl-4 -Hydroxyphenyl) sulfide composition.

[0043] S02: The petri dish is cleaned by ultrasonic for the second time, the ultrasonic power is 80W, the...

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Abstract

The invention discloses a radiation sterilization method for experimental consumables, comprising the following steps: S01: the experimental consumables are ultrasonically cleaned for the first time; S02: the experimental consumables are ultrasonically cleaned for the second time; S03: the experimental consumables are Dry in an oven at 50°C; S04: The experimental consumables are packaged; S05: The experimental consumables are 60 Cobalt gamma rays are irradiated and output, and the irradiation dose is 2‑8KGy. The present invention adopts irradiation sterilization to sterilize the radiation-resistant polypropylene experimental consumables, which can be carried out at normal temperature, and the irradiation time is short, the efficiency is high, and the sterilization effect is remarkable; The irradiated polypropylene material ensures that the performance of the experimental consumables will not change after irradiation; the invention performs two ultrasonic treatments before irradiation sterilization to remove other pollutants on the surface of the experimental consumables to further ensure the sterilization effect.

Description

technical field [0001] The invention relates to the field of radiation sterilization, in particular to a method for radiation sterilization of experimental consumables. Background technique [0002] Polypropylene is currently one of the most widely used synthetic resins at home and abroad, with the fastest growing output. Compared with other thermoplastic resins, polypropylene has the advantages of wide sources, light weight and low price, non-toxic and tasteless, high strength, and good thermal and chemical stability, so it is widely used in the field of experimental consumables. A large part of the current petri dishes and culture bottles are made of polypropylene. Petri dishes and culture bottles are common experimental consumables used for microorganism or cell culture. They usually need to be sterilized after processing to ensure that they are safe during the next use. Not affected by residual bacteria. [0003] In the process of sterilizing polypropylene-based experi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/08B08B3/12C08L23/12C08K5/3435C08K5/375
CPCA61L2/081B08B3/12C08K5/3435C08K5/375A61L2202/17C08L2201/08C08L23/12
Inventor 邹伟权
Owner 广州华大生物科技有限公司
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