Micro-fluidic chip and manufacturing method thereof

Abstract

The embodiment of the invention discloses a micro-fluidic chip and a manufacturing method thereof. The micro-fluidic chip comprises a pressurization layer comprising a vent hole and a vent groove, wherein the vent hole is used for introducing gas into the vent groove, and the vent groove generates a gas pressure based on the introduced gas; a capture layer comprising a capture channel that is usedfor introducing a plurality of samples to be pressurized; and a deformation layer arranged between the pressurization layer and the capture layer and used for generating deformation according to theair pressure so as to apply mechanical force to the plurality of samples to be pressurized in the capture layer. According to the micro-fluidic chip provided by the embodiment of the invention, cell spheres are extruded through the capture layer, the deformation layer and the pressurization layer, and the extrusion force on cells can be controlled by controlling the pressure of gas introduced by the pressurization layer, so that the controllability of cell stress is realized.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of cell culture, and in particular to a microfluidic chip and a manufacturing method thereof. Background technique [0002] In cell culture, it is necessary to simulate the living environment of cells in animals and plants under external conditions, such as the force that cells receive in the growth environment (called the mechanical characteristics of cells), so as to study the growth and differentiation of cells. [0003] The commonly used methods of cultivating cells by using mechanical properties mainly include culturing cells by using soft and hard media and cultivating cells by using microfluidic technology to simulate cell mechanical forces. Cultivating cells with soft and hard media refers to providing different mechanical support or extrusion to the cells through the hardness of the medium, but the mechanical support or extrusion of this culture method is not the same as th...

AI Technical Summary

Problems solved by technology

Cultivating cells with soft and hard media refers to providing different mechanical support or extrusion to the cells through the hardness of the medium, but the mechanical support or extrusion of this culture method is not the same as the environment of the cells in the body, resulting in The survival time and function of the resulting cells are inconsistent with the state in vivo, and it is impossible to explore the growth and differentiation process of cells close to the real biochemical environment

Using microfluidic technology to simulate cell mechanical force to cultivate cells is a culture method, usually a single-layer microfluidic technology, which is difficult to achieve quantitative control of mechanical force

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