S-methylmethionine-rich brassica oleracea plant
A technology of methylmethionine and plants, applied in the field of cabbage plants, can solve the problems of undisclosed enhancement of cruciferous plants, etc.
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[0090] Hereinafter, although it demonstrates in more detail based on an Example and a comparative example, this invention is not limited to these examples.
[0091]
[0092] After sowing, seedlings are raised in seedling trays, and after developing into seedlings suitable for planting, they are planted in the field. After conventional cultivation and management, flower buds are harvested when the flower buds reach the appropriate harvest time, and the harvested products are stored in a freezer.
[0093]
[0094] The above plant samples were homogenized using 2% phosphoric acid. This homogeneous substance was centrifuged (3000 rpm x 10 minutes), and the supernatant was obtained as an S-methylmethionine extract. The S-methylmethionine contained in the supernatant was reacted with an amino acid fluorescent labeling reagent NBD-F (4-Fluoro-7-nitrobenzofurazan) (60° C., 5 minutes) for fluorescent derivatization to prepare a measurement sample. This measurement sample was analy...
Embodiment 1
[0101] The inventors of the present invention obtained one of the double haploid lines obtained by crossing broccoli line 1 and line 2 with the offspring obtained by crossing broccoli line 3 and fixing them by anther culture Broccoli line 4-1 "SSC-BRO-17-002".
Embodiment 2
[0105] In order to confirm the content change of broccoli containing S-methylmethionine in broccoli plants, sowing was carried out in field 1 or field 2 from August 2012 to September 2012, and S-methylmethionine was tested for the 4 varieties planted. The component analysis of , confirmed the seasonal variation. The results are shown in Table 2. Flower buds were used as measurement sites. The new F1 variety described in Example 1 shows a very high content of S-methylmethionine compared with other existing broccoli varieties.
[0106] [Table 2]
[0107]
PUM
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