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Molecular marker of rice brown planthopper-resistant gene BPH39 and application thereof

An anti-brown planthopper and molecular marker technology, applied in the field of molecular genetics, can solve problems such as differentiation, and achieve the effects of easy detection, high primer specificity, and production cost savings

Pending Publication Date: 2021-03-30
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These genes may be alleles or different genes, but there is not yet enough evidence to tell them apart

Method used

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  • Molecular marker of rice brown planthopper-resistant gene BPH39 and application thereof
  • Molecular marker of rice brown planthopper-resistant gene BPH39 and application thereof
  • Molecular marker of rice brown planthopper-resistant gene BPH39 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: BPH39 Preliminary positioning and effect verification of

[0040] 1. ZS / PK BC 1 f 2 Population construction and phenotyping

[0041] (1) The Indian insect-resistant parent PAEDAI KALIBUNGGA (referred to as PK, IRGC number: 38545) is from the International Rice Research Institute (IRRI). The other parent Zhenshan 97B (ZS) used in this experiment showed high susceptibility to the brown planthopper population in my country. In order to locate the brown planthopper resistance gene in PK, the present invention crosses ZS with PK, and the obtained F 1 Selfing again, get F 2 Separate groups. then F 2 After the population was screened for resistance to brown planthopper, the resistant single plant was recrossed with ZS, and the obtained BC 1 f 1 Then self-bred to obtain BC containing 92 families 1 f 2 Separate groups; each BC 1 f 2 A single plant obtains the corresponding BC through selfing 1 f 2:3 family lineage.

[0042] (2) The improved group ident...

Embodiment 2

[0057] Example 2: BPH39 fine positioning of

[0058] 1 BPH39 Construction of near-isogenic lines and effect evaluation of

[0059] In order to fine-tune the gene and to construct a molecular marker-assisted selection system for the gene for insect resistance breeding, we constructed BPH39 A near-isogenic line against the Zhenshan 97 background. The specific steps are, first use the BPH39 flanked by molecular markers I369 and I992 (Table 3) in BC 1 f 3 family selection BPH39 The insect-resistant individual plant whose locus is the PK genotype of the homozygous insect-resistant parent, backcrossed with ZS to obtain BC 2 f 1 , then use ZS with BPH39 locus heterozygous BC 2 f 1 Further backcrossing to get BC 3 f 1 ,choose BPH39 BC 3 f 2 , and then select BPH39 The locus is a single plant of homozygous PK genotype, and then selfed to obtain BC 3 f 3 ,Right now BPH39 The near isogenic line (NIL-BPH39). We measured the seedling resistance and N. lugens honeydew a...

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Abstract

The invention provides a molecular marker of a rice brown planthopper-resistant gene BPH39 and an application thereof. A local high-density physical map covering the BPH39 is constructed by constructing a near-isogenic line of the BPH39 and analyzing a recombinant exchange single plant of a high-generation backcross population, and the gene is finely positioned in an interval of 30kb. The two molecular markers ZL1607 and ZL2137 in the interval are closest to the BPH39, and the reliability of a single plant for screening a target gene by utilizing the two markers is the highest. Besides, othermolecular markers, I1540, I32-4 and ZL6061 which are closely linked with the gene can be used for screening single plants of the target gene, so that the selection efficiency of brown planthopper-resistant rice varieties or strains can be greatly improved, and the breeding age limit is shortened.

Description

technical field [0001] The present invention belongs to the field of molecular genetics. Specifically related to a rice brown planthopper resistance gene BPH39 Closely linked molecular markers, and the application of the molecular markers in rice brown planthopper resistance breeding and variety improvement. Background technique [0002] Rice ( Oryza sativa ) is one of the most important food crops in the world, the brown planthopper ( Nilaparvata lugens Stål, BPH) is the most serious pest in rice-growing areas. With the increasing severity and frequency of pest outbreaks in the past few decades, they pose a serious threat to rice production (Wu et al. 2020 Mol Breeding 40:24). Global warming has increased the population growth and metabolic rate of pests, and environmental degradation may reduce the number of natural enemies of pests, which is expected to further exacerbate crop losses (Deutsch et al. 2018 Science 361:916-919). Controlling pests is the key to ensu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 胡杰邹玲陈志伟吴为人
Owner FUJIAN AGRI & FORESTRY UNIV
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