Complex enzyme preparation and application thereof in fish sauce processing
A compound enzyme preparation, fish sauce technology, applied in the directions of enzymes, peptidases, hydrolase, etc., can solve problems such as reducing the quality of fish sauce, increase 1-octen-3-ol metabolites, and reduce fishy smell , the effect of reducing the content of heptanal metabolites
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Embodiment 1
[0021] A compound enzyme preparation, comprising the following components in parts by weight: 3 parts of trypsin, 2 parts of keratinase, 3 parts of Halomonas Urumqi, and 3 parts of Radiomucor elegans. The enzyme activity concentration of trypsin is 300,000 U / g; the enzyme activity concentration of keratinase is 120,000 U / g; 8 cfu; the live bacteria concentration is 3×10 in the described radiomucor fungi preparation 8 cfu.
[0022] The application of above-mentioned compound enzyme preparation in fish sauce processing; The step of described fish sauce processing comprises:
[0023] (1) Stir and pulverize the leftovers after fish processing, and add the compound enzyme preparation according to the ratio of mass ratio 1:0.1;
[0024] (2) adding salt accounting for 15% of the total weight of the material in step (1), adjusting the pH of the solution to 8.0, and making an enzymolysis fermentation liquid;
[0025] (3) Put the fermented liquid prepared in step (2) into an environm...
Embodiment 2
[0028] A compound enzyme preparation, which comprises the following components in parts by weight: 4 parts of papain, 2 parts of Halomonas Urumqi and 2 parts of Actinomucor elegans. The enzyme activity concentration of the papain is 150,000-250,000 U / g; 8 cfu; the live bacteria concentration is 2×10 in the described radiomucor jachi preparation 8 cfu.
[0029] The application of above-mentioned compound enzyme preparation in fish sauce processing; The step of described fish sauce processing comprises:
[0030] (1) Stir and pulverize the leftovers after fish processing, and add the compound enzyme preparation according to the ratio of mass ratio 1:0.05;
[0031] (2) adding salt accounting for 12% of the total weight of the material in step (1), adjusting the pH of the solution to 7.8, and making an enzymolysis fermentation liquid;
[0032] (3) Put the fermented liquid prepared in step (2) into an environment with a temperature of 33° C. to ferment for 10 days to make crude f...
Embodiment 3
[0035] A compound enzyme preparation, which comprises the following components in parts by weight: 4 parts of ficin, 4 parts of bromelain, 5 parts of Halomonas ukrumqi and 5 parts of Actinomucor elegans. The ficin enzyme activity concentration is 150,000-250,000 U / g; the bromelain enzyme activity concentration is 250,000-400,000 U / g; 8 cfu; the live bacteria concentration is 4×10 in the described radiomucor jachi preparation 8 cfu.
[0036] The application of above-mentioned compound enzyme preparation in fish sauce processing; The step of described fish sauce processing comprises:
[0037] (1) Stir and pulverize the leftovers after fish processing, and add the compound enzyme preparation according to the ratio of mass ratio 1:0.12;
[0038] (2) Add salt accounting for 18% of the total weight of the material in step (1), adjust the pH of the solution to 8.2, and make an enzymolysis fermentation liquid;
[0039] (3) Put the fermented liquid prepared in step (2) into an envir...
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