Industrial fermentation production process for improving yield of biosurfactin

Abstract

The invention discloses an industrial fermentation tank for improving the yield of biosurfactin, which comprises a tank body and a fixing base, wherein the tank body is slidably connected with the top of the fixing base, a culture solution inlet is formed in the top of the left side wall of the tank body, an air outlet is formed in the top of the right side wall of the tank body, an acid storage box is arranged on the left side of the top of the tank body, an alkaline storage box is arranged on the right side of the top of the tank body, feeding ports are formed in the tops of the alkaline storage box and the acid storage box, guide pipes are communicated with the bottoms of the alkaline storage box and the acid storage box, spray heads are communicated with the ends, extending into an inner cavity of the tank body, of the guide pipes, a driving mechanism is arranged on the tank body between the alkaline storage box and the acid storage box, an air inlet pipe is communicated to the top part of the driving mechanism, and one end, extending to the inner cavity of the tank body, of the driving mechanism is connected with a rotating shaft. The design is reasonable, and the pH staged regulation and the overflow separation surfactant removal product inhibition are organically combined so as to obtain the maximum yield and the maximum yield of the fermentation production of the biological surfactant.

Description

technical field [0001] The invention relates to the technical field of fermentation technology, in particular to an industrial fermentation production technology for increasing the yield of biosurfactant. Background technique [0002] Due to its high structural diversity, excellent surface activity, biodegradability, low toxicity, and good adaptability, biosurfactants play an important role in the fields of enhanced oil recovery, environmental bioremediation, food processing, and pharmaceuticals. play an important role. [0003] The production of biosurfactants by bioreactor fermentation is a complex biochemical reaction process, which usually requires ventilation and stirring. In the fermentation production process, effective oxygen supply is an important factor, which not only affects the fermentation yield, but also may affect the product type. The carrying out of fermentation, the biosurfactant amphiphile molecule that produces gathers at the air-liquid interface (mainl...

AI Technical Summary

Problems solved by technology

The existing technology has a constant pH of 7, which is beneficial to the growth of the strain and not conducive to the metabolism of the strain; and under the condition of uncontrolled pH fermentation, the pH quickly drops below 6.5 after entering the stable growth period, and the growth of the strain and the ability to ferment and produce biosurfactants are greatly affected. impact, and at this time the nutrient medium has not been effectively utilized completely

It can be seen that in the prior art, by foam separation alone, although the production of biosurfactant is improved compared with the fermentation production of defoaming agent, because the pH is not controlled in stages according to the physiological growth and metabolism characteristics of the strain, there is a fermentation capacity of the production strain Deficiencies of underutilization and underutilization of the nutrient medium

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