Preparation method of spinal cord subacute combined degeneration model

Abstract

The invention discloses a preparation method of a spinal cord subacute combined degenerative disease model, which comprises diluting 10 μl of hexachlorobutadiene into 490 μl of corn oil; according to the dose of 150-250 μl / mouse, intragastric or intraperitoneal administration of the mice Injection, normal feeding during the medication period; intragastric or intraperitoneal injection again at intervals of 48 hours, a total of three administrations; tests on mice to observe whether clinical symptoms of subacute combined degenerative disease of the spinal cord appear, including the following methods: Medication During the period, the weight of the mice was tested and recorded every 24 hours, and the behavioral evaluation of the mice was carried out, including but not limited to: gait, pole climbing, rotating rod and wire grid experiments, and the mice were measured after the medication was completed. serum vitamin B 12 content. Through the above settings, the preparation method of the spinal cord subacute combined degenerative disease model can be completed within 6 days using HCBD, and because the price of HCBD is low, the preparation method of the present application has advantages compared with the traditional preparation method. Advantages of high speed and low cost.

Description

technical field [0001] The invention relates to the technical field of medical biology, in particular to a method for preparing a spinal cord subacute combined degenerative disease model. Background technique [0002] Subacute combined degeneration of the spinal cord, referred to as subacute combined degeneration, is due to vitamin B 12 Impaired intake, absorption, binding, transport, or metabolism of B vitamins in the body 12 Diseases of central and peripheral nervous system degeneration caused by insufficient content. The lesions mainly involve the dorsal cord, lateral cord, and peripheral nerves. The clinical manifestations are deep sensory loss of the lower extremities, sensory ataxia, spastic paralysis, and peripheral neuropathy, often accompanied by clinical signs of anemia. [0003] In order to better study the subacute combined degeneration of the spinal cord, it is necessary to establish corresponding animal models. Animal disease models are mainly used in experi...

AI Technical Summary

Problems solved by technology

[0004] The existing animal model of subacute combined degeneration of the spinal cord is prepared as follows: through manipulation at the genetic level, the mice cannot complete the vitamin B 12 intake, thus making vitamin B in mice 12 Subacute combined degeneration of the spinal cord

However, the transgenic mice used in this preparation method are expensive and are not sold in China. At the same time, considering that the preparation cycle is about two months, this preparation method has the disadvantages of long preparation time and high preparation cost.

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