Negative pressure culture device convenient for collagen extraction

Abstract

The invention relates to the technical field of cell tissue culture devices, and particularly relates to a negative pressure culture device convenient for collagen extraction. The negative pressure culture device comprises a shell, wherein the shell is connected with a cover plate; a partition plate is arranged in the shell; a sealing ring is arranged below the partition plate; a placement hole is formed in the partition plate; a bearing and a limiting rod are arranged on the bottom surface of the shell; the bearing is connected with a rotary column; and the rotary column is connected with a plugging block, a culture dish and a tray. A culture solution is added into a storage tank and reaches a liquid outlet through a conveying pipe and a hose, an electric push rod pushes the liquid outlet to the upper part of the culture dish, and the culture solution can be added into the culture dish under the condition that the internal space of the device is not opened, so that the device is more convenient and practical; and when the culture dish is taken and stored, only the rotary column needs to be rotated, so that the tray drives the culture dish to move upwards and jacks the plugging block open, the placement hole is always in a plugged state, the interior of the device can be still in a relatively closed state, the internal pressure cannot be greatly changed, and a good culture environment is kept.

Description

technical field [0001] The invention relates to the technical field of cell tissue culture devices, in particular to a negative pressure culture device for facilitating the extraction of collagen. Background technique [0002] Collagen is a biological polymer, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and some organisms even reach more than 80% . Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity. [0003] In order to facilitate the extraction of a small amount of collagen at any time for research experiments, the laboratory will culture tissue cells from which collagen can be extracted in a negative pressure culture device. [0004] However, the existing devices must be opened when adding culture fluid to the petri dish and ac...

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Problems solved by technology

[0004] However, the existing device must be opened when adding culture fluid to the petri dish and accessing the petri dish, so that the internal pressure of the device will rise instantaneously, and the negative pressure effect will be lost, and the frequent opening of the device will allow dust and bacteria in the air to enter the device Inside, contaminate the Petri dish

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