Bioreactor and bioreaction system with the bioreactor

A bioreactor, biomechanical technology, applied in the field of tissue engineering, can solve the problems of low integration, simultaneous realization of pulsatile perfusion and mechanical excitation, etc.

Pending Publication Date: 2021-04-09
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the above-mentioned problems of low integration of existing bioreactors and the inability to realize the functions of pulsating perfusion and mechanical excitation at the same time, the present invention provides a bioreactor

Method used

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  • Bioreactor and bioreaction system with the bioreactor
  • Bioreactor and bioreaction system with the bioreactor
  • Bioreactor and bioreaction system with the bioreactor

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Such as Figure 1 to Figure 3 As shown, a bioreactor, which includes a mechanical generation chamber 1, a circulation culture chamber 2, and a pulsating flow generation chamber 3 that are detachably connected in sequence from top to bottom, and the bottom of the circulation culture chamber 2 and the pulsation flow generation chamber Inside the chamber 3 is provided a pulsating flow generating device for providing a physiological pulsating flow physiological environment for the tissue to be cultured, and the side wall of the circulation culture chamber 2 is provided with a culture solution inlet 4 and a culture solution outlet for the culture solution to enter and exit. In this way, the mechanical generation chamber 1 can simulate the biomechanical environment required by the muscle tissue when cultivating the muscle tissue; the circulation culture chamber 2 is used as a general dynamic culture, and the culture fluid inlet 4 and the culture fluid outlet are connected with...

Embodiment 2

[0045] This embodiment is similar to Embodiment 1, the difference is that, as Figure 9 As shown, the mechanical generating chamber 1 is a stirring mechanical generating chamber 1, and a stirring module is arranged inside the stirring mechanical generating chamber 1, and the stirring module extends into the circulation through the bottom of the stirring mechanical generating chamber 1. The interior of culture room 2.

[0046] Such as Figure 9As shown, the stirring mechanism generation chamber 1 includes a first top cover 101, and the stirring module includes a first bottom plate 102 arranged at the bottom of the first top cover 101, and is arranged on the first bottom plate 102 close to the first bottom plate 102. The second stepper motor 130 on the side of a top cover 101 and the second control board 131 connected thereto, the output end of the second stepper motor 130 is provided with a connecting bar 132, and the connecting bar 132 passes through The first bottom plate 1...

Embodiment 3

[0049] like Figure 10 Shown, a kind of bioreaction system, wherein, comprises bioreactor 7, peristaltic pump 8 and liquid storage bottle 9, and described bioreactor 7, peristaltic pump 8 and liquid storage bottle 9 are connected end-to-end through pipeline to form circulation loop , the bioreactor 7 is the bioreactor 7 described in embodiment 1, the pipelines on both sides of the bioreactor 7 are respectively connected to the culture solution inlet 4 and the culture solution outlet on the side wall of the circulation culture chamber 2 connect. The culture is placed in the bioreactor 7, and the culture solution in the pipeline driven by the peristaltic pump 8 flows through the bioreactor 7, and the culture solution is housed in the liquid storage bottle 9, which is used to update the culture solution and belt in the bioreactor 7. Take away the waste produced by cultivation.

[0050] The working principle of this embodiment: the liquid storage bottle 9 is loaded with culture ...

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PUM

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Abstract

The invention provides a bioreactor. The bioreactor comprises a mechanics generation chamber, a circulating culture chamber and a pulsating flow generation chamber which are detachably connected in sequence from top to bottom, a pulsating flow generation device is arranged at the bottom of the circulating culture chamber and in the pulsating flow generation chamber, and a culture solution inlet and a culture solution outlet for a culture solution to enter and exit are formed in the side wall of the circulating culture chamber; and the mechanics generation chamber is provided with a mechanics module used for providing a biomechanics simulation environment for to-be-cultured tissues. The invention also provides a bioreaction system with the bioreactor. The invention is high in integration degree, capable of achieving functions of pulsating perfusion and mechanics stimulus at the same time, ingenious in connecting structures between the chambers and convenient to disassemble to take out culture.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, and more specifically, to a bioreactor and a bioreaction system with the bioreactor. Background technique [0002] In the field of tissue engineering, tissue engineering bioreactor is an important technical means to study the influence of different environmental factors (physical and biochemical factors) on the three-dimensional functional culture of specific cells and tissues. Automation, online monitoring, etc.), making tissue engineering a key link in the process of entering standard industrial scale production and clinical application from the laboratory. With the development of bio-3D printing technology, it is necessary to develop advanced bioreactors for tissue engineering to cultivate the "cell-scaffold mixture" printed by bioprinters. The bio-ink used for bio-printing is a jelly-like substance mixed with hydrogel and cells. It is printed through a special low-temperature nozz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/02C12M1/00
CPCC12M21/08C12M23/44C12M25/00C12M27/02C12M29/12C12M29/18C12M35/04
Inventor 钟华强张传杰袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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